Navegar

Colección

Datos Estadísticas

Artículo

Trelles, J.A.; Bentancor, L.; Schoijet, A.; Porro, S.; Lewkowicz, E.S.; Sinisterra, J.V.; Iribarren, A.M. "Immobilized escherichia coli BL21 as a catalyst for the synthesis of adenine and hypoxanthine nucleosides" (2004) Chemistry and Biodiversity. 1(2):280-288
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Different supports, such as alginate, agar, agarose, and polyacrylamide, were used to immobilize Escherichia coli BL 21 by entrapment techniques. The transglycosylation reaction involved in the synthesis of adenosine from uridine and adenine was chosen as a model system to study the characteristics of these biocatalysts. Whole cells immobilized on agarose proved to be optimal and could be used up to 30 times without significant loss of activity. This biocatalyst was further employed to test its ability in the synthesis of other adenine and hypoxanthine nucleosides. Ribo-, 2′-deoxyribo-, and arabinonucleosides could be prepared in high yields starting from the corresponding pyrimidine nucleosides and purine bases. Similar product yields were obtained with both free and immobilized cells, though, in the latter case, a longer reaction time was necessary.

Registro:

Documento: Artículo
Título:Immobilized escherichia coli BL21 as a catalyst for the synthesis of adenine and hypoxanthine nucleosides
Autor:Trelles, J.A.; Bentancor, L.; Schoijet, A.; Porro, S.; Lewkowicz, E.S.; Sinisterra, J.V.; Iribarren, A.M.
Filiación:Universidad Nacional de Quilmes, R.S. Peña 180, (1876) Bernal, Buenos Aires, Argentina
Departamento de Química Orgánica y Farmaceutica, Facultad de Farmacia, Universidad Complutense de Madrid, Ciudad Universitaria s/n, E-28040 Madrid, Spain
INGEBI, CONICET, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
Palabras clave:adenine; Escherichia coli protein; hypoxanthine; nucleoside; article; biosynthesis; catalysis; chemistry; Escherichia coli; metabolism; Adenine; Catalysis; Escherichia coli; Escherichia coli Proteins; Hypoxanthine; Nucleosides
Año:2004
Volumen:1
Número:2
Página de inicio:280
Página de fin:288
DOI: http://dx.doi.org/10.1002/cbdv.200490024
Título revista:Chemistry and Biodiversity
Título revista abreviado:Chem. Biodiversity
ISSN:16121872
CODEN:CBHIA
CAS:adenine, 22177-51-1, 2922-28-3, 73-24-5; hypoxanthine, 68-94-0; Adenine, 73-24-5; Escherichia coli Proteins; Hypoxanthine, 68-94-0; Nucleosides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16121872_v1_n2_p280_Trelles

Referencias:

  • Garg, R., Gupta, S., Gao, H., Babu, M., Debnath, A., Hansch, C., (1999) Chem. Rev., 99, p. 3523
  • Holy, A., (2001) Cas Lek Cesk., 140, p. 583
  • Ichikawa, E., Kato, K., (2001) Curr. Med. Chem., 8, p. 385
  • Giri, A., Dhingra, V., Giri, C.C., Singh, A., Ward, O.P., Narasu, M.L., (2001) Biotechnol. Adv., 19, p. 175
  • Gotor, V., (2002) J. Biotechnol., 96, p. 35
  • Cheetham, P.S.J., (1998) J. Biotechnol., 66, p. 3
  • Prasad, A.K., Trikha, S., Parmar, V.S., (1999) Bioorg. Chem., 27, p. 135
  • Utagawa, T., (1999) J. Mol. Catal. B: Enzym., 6, p. 215
  • Lewkowicz, E., Martínez, N., Rogert, M.C., Porro, S., Iribarren, A.M., (2000) Biotechnol. Lett., 22, p. 1277
  • Rogert, M.C., Trelles, J.A., Porro, S., Lewkowicz, E.S., Iribarren, A.M., (2002) Biocatal. Biotransform., 20, p. 347
  • Murakami, K., Shirasaka, T., Yoshioka, H., Kojima, E., Aoki, S., Ford, H., Driscoll, J., Mitsuya, H., (1991) J. Med. Chem., 34, p. 1606
  • Yokozeki, K., Tsuji, T., (2000) J. Mol. Catal. B: Enzym., 10, p. 207
  • Bickerstaff, G.F., (1997) Immobilization of Enzymes and Cells, p. 1. , Ed. G. F. Bickerstaff, Humana Press, Inc., Totowa, NJ
  • Hulst, A.C., Tramper, J., Riet, K., Westerbeek, J.M.M., (1985) Biotechnol. Bioeng., 27, p. 870
  • Quintana, M.G., Dalton, H., (1999) Enzyme Microb. Technol., 24, p. 232
  • Sinisterra, J.V., Dalton, H., (1996) Immobilized Cells: Basics and Applications, p. 416. , Eds. R. H. Wijffels, R. M. Buitelaar, C. Bucke, J. Tramper, Elsevier, Amsterdam, New York, Oxford, Tokyo
  • Llanes, N., Fernandes, P., Leon, R., Cabral, J.M.S., Pinheiro, H.M., (2001) J. Mol. Catal. B: Enzym., 11, p. 523
  • Demain, A.L., (2000) Trends Biotechnol., 18, p. 26
  • Van Del Sluis, C., Mulder, A.N.T., Grolle, C.F., Engbers, G.H.M., Schure, E.G., Tramper, J., Wijffels, R.H., (2000) J. Biotechnol., 80, p. 179
  • Mazid, M.A., (1993) Biotechnology, 11, p. 690
  • Khattar, J.I.S., Sarma, T.A., Singh, T.A.D.P., (1999) Enzyme Microb. Technol., 25, p. 564
  • Vanek, T., Valterova, I., Vankova, R., Vaisar, T., (1999) Biotechnol. Lett., 21, p. 625
  • Chang, J.S., Chou, C., Chen, S.Y., (2001) Process Biochem., 36, p. 757
  • Lozinsky, V.I., Plieva, F.M., (1998) Enzyme Microb. Technol., 23, p. 227
  • Nilsson, K., Birnbaum, S., Flygare, S., Linse, L., Schroder, U., Jeppson, U., Larsson, P.O., Brodelius, P., (1983) Eur. J. Appl. Microbiol. Biotechnol., 17, p. 319
  • Gaevaia, L.V., Zhukov, V.N., (1987) Prikl Biokhim Mikrobiol., 23, p. 309
  • Hori, N., Watanabe, M., Sunagawa, K., Uehara, K., Mikami, Y., (1991) J. Biotechnol., 17, p. 121
  • Holy, A., Votruba, I., (1987) Nucleic Acids Symp. Ser., 18, p. 69
  • Ewing, D.F., Holy, A., Votruba, I., Humble, R.W., Mackenzie, G., Hewedi, F., Shaw, G., (1991) Carbohydr. Res., 21, p. 109
  • Iaskovich, G.A., Iakovleva, E.P., (1999) Prikl Biokhim Mikrobiol., 35, p. 146
  • Trelles, J.A., Fernández, M., Lewkowicz, E.S., Iribarren, A.M., Sinisterra, J.V., (2003) Tetrahedron Lett., 44, p. 2605

Citas:

---------- APA ----------
Trelles, J.A., Bentancor, L., Schoijet, A., Porro, S., Lewkowicz, E.S., Sinisterra, J.V. & Iribarren, A.M. (2004) . Immobilized escherichia coli BL21 as a catalyst for the synthesis of adenine and hypoxanthine nucleosides. Chemistry and Biodiversity, 1(2), 280-288.
http://dx.doi.org/10.1002/cbdv.200490024
---------- CHICAGO ----------
Trelles, J.A., Bentancor, L., Schoijet, A., Porro, S., Lewkowicz, E.S., Sinisterra, J.V., et al. "Immobilized escherichia coli BL21 as a catalyst for the synthesis of adenine and hypoxanthine nucleosides" . Chemistry and Biodiversity 1, no. 2 (2004) : 280-288.
http://dx.doi.org/10.1002/cbdv.200490024
---------- MLA ----------
Trelles, J.A., Bentancor, L., Schoijet, A., Porro, S., Lewkowicz, E.S., Sinisterra, J.V., et al. "Immobilized escherichia coli BL21 as a catalyst for the synthesis of adenine and hypoxanthine nucleosides" . Chemistry and Biodiversity, vol. 1, no. 2, 2004, pp. 280-288.
http://dx.doi.org/10.1002/cbdv.200490024
---------- VANCOUVER ----------
Trelles, J.A., Bentancor, L., Schoijet, A., Porro, S., Lewkowicz, E.S., Sinisterra, J.V., et al. Immobilized escherichia coli BL21 as a catalyst for the synthesis of adenine and hypoxanthine nucleosides. Chem. Biodiversity. 2004;1(2):280-288.
http://dx.doi.org/10.1002/cbdv.200490024