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Nunes-Nesi, A.; Alseekh, S.; de Oliveira Silva, F.M.; Omranian, N.; Lichtenstein, G.; Mirnezhad, M.; González, R.R.R.; Garcia, J.S.; Conte, M.; Leiss, K.A.; Klinkhamer, P.G.L.; Nikoloski, Z.; Carrari, F.; Fernie, A.R. "Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds" (2019) Metabolomics. 15(4)
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Abstract:

Introduction: To date, most studies of natural variation and metabolite quantitative trait loci (mQTL) in tomato have focused on fruit metabolism, leaving aside the identification of genomic regions involved in the regulation of leaf metabolism. Objective: This study was conducted to identify leaf mQTL in tomato and to assess the association of leaf metabolites and physiological traits with the metabolite levels from other tissues. Methods: The analysis of components of leaf metabolism was performed by phenotypying 76 tomato ILs with chromosome segments of the wild species Solanum pennellii in the genetic background of a cultivated tomato (S. lycopersicum) variety M82. The plants were cultivated in two different environments in independent years and samples were harvested from mature leaves of non-flowering plants at the middle of the light period. The non-targeted metabolite profiling was obtained by gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). With the data set obtained in this study and already published metabolomics data from seed and fruit, we performed QTL mapping, heritability and correlation analyses. Results: Changes in metabolite contents were evident in the ILs that are potentially important with respect to stress responses and plant physiology. By analyzing the obtained data, we identified 42 positive and 76 negative mQTL involved in carbon and nitrogen metabolism. Conclusions: Overall, these findings allowed the identification of S. lycopersicum genome regions involved in the regulation of leaf primary carbon and nitrogen metabolism, as well as the association of leaf metabolites with metabolites from seeds and fruits. © 2019, The Author(s).

Registro:

Documento: Artículo
Título:Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
Autor:Nunes-Nesi, A.; Alseekh, S.; de Oliveira Silva, F.M.; Omranian, N.; Lichtenstein, G.; Mirnezhad, M.; González, R.R.R.; Garcia, J.S.; Conte, M.; Leiss, K.A.; Klinkhamer, P.G.L.; Nikoloski, Z.; Carrari, F.; Fernie, A.R.
Filiación:Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa, Minas Gerais 36570-900, Brazil
Max-Planck-Institute of Molecular Plant Physiology, Am Mühlenberg 1, Potsdam, Golm, OT 14476, Germany
Center of Plant System Biology and Biotechnology (CPSBB), Plovdiv, Bulgaria
Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaría, Consejo Nacional de Investigaciones Científicas y Técnicas, Castelar, B1712WAA, Argentina
Plant Ecology, Institute of Biology, Leiden University, Sylviusweg 72, Leiden, 2333 BE, Netherlands
Bioinformatics Group, Institute of Biochemistry and Biology, University of Potsdam, Potsdam, Germany
Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Universidad de Buenos Aires, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
Facultad de Agronomía, Cátedra de Genética, Universidad de Buenos Aires, Buenos Aires, Argentina
Business Unit Horticulture, Wageningen University & Research, Postbus 20, Bleiswijk, 2665 ZG, Netherlands
Palabras clave:Leaf metabolism; Metabolite network; Metabolite QTL; Tomato; alanine; aspartic acid; fructose; glucose; glutamic acid; glyceric acid; glycine; isoleucine; leucine; nitrogen; proline; psicose; serine; sucrose; tyrosine; allele; amino acid analysis; Article; biotic stress; electron transport; gas chromatography; gene expression; gene location; genetic background; genetic variation; genotype; harvest index; mass fragmentography; metabolite; metabolomics; nonhuman; phenotype; photosynthesis; plant gene; plant growth; plant leaf; quantitative trait locus; time of flight mass spectrometry; tomato
Año:2019
Volumen:15
Número:4
DOI: http://dx.doi.org/10.1007/s11306-019-1503-8
Título revista:Metabolomics
Título revista abreviado:Metabolomics
ISSN:15733882
CAS:alanine, 56-41-7, 6898-94-8; aspartic acid, 56-84-8, 6899-03-2; fructose, 30237-26-4, 57-48-7, 7660-25-5, 77907-44-9; glucose, 50-99-7, 84778-64-3; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; glyceric acid, 473-81-4; glycine, 56-40-6, 6000-43-7, 6000-44-8; isoleucine, 7004-09-3, 73-32-5; leucine, 61-90-5, 7005-03-0; nitrogen, 7727-37-9; proline, 147-85-3, 7005-20-1; psicose, 23140-52-5; serine, 56-45-1, 6898-95-9; sucrose, 122880-25-5, 57-50-1; tyrosine, 16870-43-2, 55520-40-6, 60-18-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15733882_v15_n4_p_NunesNesi

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Citas:

---------- APA ----------
Nunes-Nesi, A., Alseekh, S., de Oliveira Silva, F.M., Omranian, N., Lichtenstein, G., Mirnezhad, M., González, R.R.R.,..., Fernie, A.R. (2019) . Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds. Metabolomics, 15(4).
http://dx.doi.org/10.1007/s11306-019-1503-8
---------- CHICAGO ----------
Nunes-Nesi, A., Alseekh, S., de Oliveira Silva, F.M., Omranian, N., Lichtenstein, G., Mirnezhad, M., et al. "Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds" . Metabolomics 15, no. 4 (2019).
http://dx.doi.org/10.1007/s11306-019-1503-8
---------- MLA ----------
Nunes-Nesi, A., Alseekh, S., de Oliveira Silva, F.M., Omranian, N., Lichtenstein, G., Mirnezhad, M., et al. "Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds" . Metabolomics, vol. 15, no. 4, 2019.
http://dx.doi.org/10.1007/s11306-019-1503-8
---------- VANCOUVER ----------
Nunes-Nesi, A., Alseekh, S., de Oliveira Silva, F.M., Omranian, N., Lichtenstein, G., Mirnezhad, M., et al. Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds. Metabolomics. 2019;15(4).
http://dx.doi.org/10.1007/s11306-019-1503-8