Artículo
Flórez-Castillo, J.M.; Perullini, M.; Jobbágy, M.; De Jesús Cano Calle, H. "Enhancing antibacterial activity against Escherichia coli K-12 of peptide Ib-AMP4 with synthetic analogues" (2014) International Journal of Peptide Research and Therapeutics. 20(3):365-369
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Abstract:
A family of Ib-AMP4 peptide analogues was obtained by solid phase synthesis, modifying the net charge and hydrophobicity of C-terminal domain by replacing certain amino acidic residues by arginine and tryptophan. Additionally, disulfide bonds were eliminated by replacing the cysteine residues by methionine, which resulted in a decrease in the number of synthesis byproducts, and consequently diminished the subsequent purification steps. The obtained peptides were purified by RP-HPLC and their molecular mass was determined by MALDI-TOF mass spectrometry. The peptide analogues (IC 50 between 1 and 50 μM) presented a higher antibacterial activity against Escherichia coli K-12 than the native peptide (IC50 > 100 μM). The hemolytic activity of the peptide with the highest antibacterial efficacy presented no degradation of erythrocytes for a concentration of 1 μM that corresponds to its IC50 value. The results show that the synthesized peptides are good candidates for the treatment of diseases caused by E. coli. © 2014 Springer Science+Business Media.
Registro:
| Documento: | Artículo |
| Título: | Enhancing antibacterial activity against Escherichia coli K-12 of peptide Ib-AMP4 with synthetic analogues |
| Autor: | Flórez-Castillo, J.M.; Perullini, M.; Jobbágy, M.; De Jesús Cano Calle, H. |
| Filiación: | Grupo de Investigación en Bioquímica Y Microbiología - GIBIM, Universidad Industrial de Santander, Carrera 27 Calle 9, Bucaramanga, Santander, Colombia Instituto de Química Física de Materiales, Medio Ambiente Y Energía - INQUIMAE, Universidad de Buenos Aires, Ciudad Universitaria Pabellón 2, 1428 Buenos Aires, Argentina |
| Palabras clave: | Antibacterial peptides; Escherichia coli; Ib-AMP; Modified peptides; arginine; cysteine; disulfide; Ib AMP4 peptide; Ib AMP4 peptide derivative; Ib M1 peptide; Ib M2 peptide; Ib M3 peptide; Ib M4 peptide; Ib M5 peptide; Ib M6 peptide; methionine; peptide derivative; polypeptide antibiotic agent; synthetic peptide; tryptophan; unclassified drug; amino acid substitution; antibacterial activity; article; carboxy terminal sequence; controlled study; disulfide bond; drug determination; drug efficacy; drug purification; drug synthesis; Escherichia coli K 12; hemolysis; human; human cell; hydrophobicity; IC 50; matrix assisted laser desorption ionization time of flight mass spectrometry; minimum inhibitory concentration; molecular weight; nonhuman; protein determination; protein domain; protein purification; protein synthesis; reversed phase high performance liquid chromatography; Escherichia coli; Escherichia coli K12 |
| Año: | 2014 |
| Volumen: | 20 |
| Número: | 3 |
| Página de inicio: | 365 |
| Página de fin: | 369 |
| DOI: | http://dx.doi.org/10.1007/s10989-014-9391-2 |
| Título revista: | International Journal of Peptide Research and Therapeutics |
| Título revista abreviado: | Int. J. Pept. Res. Ther. |
| ISSN: | 15733149 |
| CODEN: | IJPRF |
| CAS: | arginine, 1119-34-2, 15595-35-4, 7004-12-8, 74-79-3; cysteine, 4371-52-2, 52-89-1, 52-90-4; disulfide, 16734-12-6; methionine, 59-51-8, 63-68-3, 7005-18-7; tryptophan, 6912-86-3, 73-22-3 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15733149_v20_n3_p365_FlorezCastillo |
Referencias:
- Alves, C.S., Melo, M.N., Franquelim, H.G., Ferre, R., Planas, M., Feliu, L., Escherichia coli cell surface perturbation and disruption induced by antimicrobial peptides BP100 and pepR (2010) J Biol Chem, 285, pp. 27536-27544
- Bisht, G.S., Rawat, D.S., Kumar, A., Kumar, R., Pasha, S., Antimicrobial activity of rationally designed amino terminal modified peptides (2007) Bioorg Med Chem Lett, 17, pp. 4343-4346
- Čeřovský, V., Slaninová, J., Fučík, V., Hulačová, H., Borovičková, L., Ježek, R., New potent antimicrobial peptides from the venom of Polistinae wasps and their analogs (2008) Peptides, 29, pp. 992-1003
- Chan, D.I., Prenner, E.J., Vogel, H.J., Tryptophan- and arginine-rich antimicrobial peptides: Structures and mechanisms of action (2006) Membr Biophys Antimicrob Pept, 1758, pp. 1184-1202
- De Luca, A.J., Walsh, T.J., Antifungal peptides: Origin, activity, and therapeutic potential (2000) Rev Iberoam Micol, 17, pp. 116-120
- Epand, R.M., Vogel, H.J., Diversity of antimicrobial peptides and their mechanisms of action (1999) Biochim Biophys Acta, 1462, pp. 11-28
- Franco, O.L., Murad, A.M., Leite, J.R., Mendes, P.A.M., Prates, M.V., Bloch, C., Identification of a cowpea γ-thionin with bactericidal activity (2006) FEBS J, 273, pp. 3489-3497
- Haney, E.F., Nazmi, K., Lau, F., Bolscher, J.G.M., Vogel, H.J., Novel lactoferrampin antimicrobial peptides derived from human lactoferrin (2009) Biochimie, 91, pp. 141-154
- Helmerhorst, E.J., Reijnders, I.M., Van'T Hof, W., Veerman, E.C., Nieuw Amerongen, A.V., A critical comparison of the hemolytic and fungicidal activities of cationic antimicrobial peptides (1999) FEBS Lett, 449, pp. 105-110
- Ikeda, T., Yamaguchi, H., Tazuke, S., Phase-separation in phospholipid bilayers induced by biologically active polycations (1990) Biochim Biophys Acta, 1026, pp. 105-112
- Kamysz, W., Okroj, M., Lukasiak, J., Novel properties of antimicrobial peptides (2003) Acta Biochim Pol, 50, pp. 461-469
- Lee, D., Shin, S., Kim, D.-H., Seo, M., Kang, J., Lee, Y., Antifungal mechanism of a cysteine-rich antimicrobial peptide, Ib-AMP1, from Impatiens balsamina against Candida albicans (1999) Biotechnol Lett, 21, pp. 1047-1050
- Marr, A.K., Gooderham, W.J., Hancock, R.E., Antibacterial peptides for therapeutic use: Obstacles and realistic outlook (2006) Anti Infect Technol, 6, pp. 468-472
- Pelegrini, P.B., Murad, A.M., Silva, L.P., Dos Santos, R.C.P., Costa, F.T., Tagliari, P.D., Identification of a novel storage glycine-rich peptide from guava (Psidium guajava) seeds with activity against Gram-negative bacteria (2008) Peptides, 29, pp. 1271-1279
- Reddy, K.V.R., Yedery, R.D., Aranha, C., Antimicrobial peptides: Premises and promises (2004) Int J Antimicrob Ag, 24 (6), pp. 536-547
- Selitrennikoff, C.P., Antifungal Proteins (2001) Appl Environ Microbiol, 67 (7), pp. 2883-2894
- Tailor, R.H., Acland, D.P., Attenborough, S., Cammue, B.P.A., Evans, I.J., Osborn, R.W., A Novel Family of Small Cysteine-rich Antimicrobial Peptides from Seed of Impatiens balsamina Is Derived from a Single Precursor Protein (1997) J Biol Chem, 272 (39), pp. 24480-24487
- Thevissen, K., FranÃois, I.E.J.A., Sijtsma, L., Amerongen, Av., Schaaper, W.M.M., Meloen, R., Posthuma-Trumpie, T., Cammue, B.P.A., Antifungal activity of synthetic peptides derived from Impatiens balsamina antimicrobial peptides Ib-AMP1 and Ib-AMP4 (2005) Peptides, 26 (7), pp. 1113-1119
- Van Der Kraan, M.I.A., Van Der Made, C., Van't Hof W, N.K., Groenink, J., Veerman, E.C.I., Bolscher, J.G.M., Nieuw Amerongen, A.V., Effect of amino acid substitutions on the candidacidal activity of LFampin 265-284 (2005) Peptides, 26 (11), pp. 2093-2097
- Wang, P., Bang, J.-K., Kim, H.J., Kim, J.-K., Kim, Y., Shin, S.Y., Antimicrobial specificity and mechanism of action of disulfide-removed linear analogs of the plant-derived Cys-rich antimicrobial peptide Ib-AMP1 (2009) Peptides, 30 (12), pp. 2144-2149
Citas:
---------- APA ----------
Flórez-Castillo, J.M., Perullini, M., Jobbágy, M. & De Jesús Cano Calle, H. (2014) . Enhancing antibacterial activity against Escherichia coli K-12 of peptide Ib-AMP4 with synthetic analogues. International Journal of Peptide Research and Therapeutics, 20(3), 365-369.http://dx.doi.org/10.1007/s10989-014-9391-2
---------- CHICAGO ----------
Flórez-Castillo, J.M., Perullini, M., Jobbágy, M., De Jesús Cano Calle, H. "Enhancing antibacterial activity against Escherichia coli K-12 of peptide Ib-AMP4 with synthetic analogues" . International Journal of Peptide Research and Therapeutics 20, no. 3 (2014) : 365-369.http://dx.doi.org/10.1007/s10989-014-9391-2
---------- MLA ----------
Flórez-Castillo, J.M., Perullini, M., Jobbágy, M., De Jesús Cano Calle, H. "Enhancing antibacterial activity against Escherichia coli K-12 of peptide Ib-AMP4 with synthetic analogues" . International Journal of Peptide Research and Therapeutics, vol. 20, no. 3, 2014, pp. 365-369.http://dx.doi.org/10.1007/s10989-014-9391-2
---------- VANCOUVER ----------
Flórez-Castillo, J.M., Perullini, M., Jobbágy, M., De Jesús Cano Calle, H. Enhancing antibacterial activity against Escherichia coli K-12 of peptide Ib-AMP4 with synthetic analogues. Int. J. Pept. Res. Ther. 2014;20(3):365-369.http://dx.doi.org/10.1007/s10989-014-9391-2
