Artículo
Flexer, V.; Calvo, E.J.; Bartlett, P.N. "The application of the relaxation and simplex method to the analysis of data for glucose electrodes based on glucose oxidase immobilised in an osmium redox polymer" (2010) Journal of Electroanalytical Chemistry. 646(1-2):24-32
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Abstract:
Data for a series of fully integrated glucose oxidase, osmium redox polyelectrolyte layers deposited on thiolated gold electrodes by layer-by-layer self assembly was analysed using the relaxation and simplex method described in our earlier work (Flexer et al., 2008) [12]. The layer-by-layer assembly method allows fine control over the film thickness, enzyme loading, osmium and glucose concentrations with good reproducibility from electrode to electrode. In the analysis we combine the use of approximate analytical expressions with digital simulation to fit the data from an extensive set of experiments. The analysis shows a thickness dependence of the fraction of "wired enzyme molecules" and second order enzyme re-oxidation rate constant for thin films (below 300 nm) following changes in the multilayer film structure. For films thicker than 300 nm the kinetic data approach that of a redox hydrogel. © 2010 Elsevier B.V. All rights reserved.
Registro:
| Documento: | Artículo |
| Título: | The application of the relaxation and simplex method to the analysis of data for glucose electrodes based on glucose oxidase immobilised in an osmium redox polymer |
| Autor: | Flexer, V.; Calvo, E.J.; Bartlett, P.N. |
| Filiación: | INQUIMAE, Departamento de Quimica Inorganica, Universidad de Buenos Aires, C1428 EHA, Argentina School of Chemistry, University of Southampton, Southampton, SO17 1BJ, United Kingdom CRPP Centre de Recherche Paul Pascal, 115 Av. Albert Schweitzer, 33600 Pessac, France |
| Palabras clave: | Enzyme electrode; Glucose oxidase; Kinetics; Layer-by-layer assembly; Osmium redox polymer; Electrodes; Enzymes; Film preparation; Glucose; Glucose sensors; Gold coatings; Multilayer films; Optimization; Osmium; Polymers; Rate constants; Analysis of data; Analytical expressions; Digital simulation; Enzyme electrode; Enzyme loading; Enzyme molecules; Fully integrated; Glucose concentration; Gold electrodes; Kinetic data; Layer by layer self assembly; Layer-by-layer assemblies; Layer-by-layer assembly; Polyelectrolyte layers; Re-oxidation; Redox hydrogels; Redox polymers; Reproducibilities; Second orders; Simplex methods; Thickness dependence; Glucose oxidase |
| Año: | 2010 |
| Volumen: | 646 |
| Número: | 1-2 |
| Página de inicio: | 24 |
| Página de fin: | 32 |
| DOI: | http://dx.doi.org/10.1016/j.jelechem.2009.11.017 |
| Título revista: | Journal of Electroanalytical Chemistry |
| Título revista abreviado: | J Electroanal Chem |
| ISSN: | 15726657 |
| CODEN: | JECHE |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15726657_v646_n1-2_p24_Flexer |
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Citas:
---------- APA ----------
Flexer, V., Calvo, E.J. & Bartlett, P.N. (2010) . The application of the relaxation and simplex method to the analysis of data for glucose electrodes based on glucose oxidase immobilised in an osmium redox polymer. Journal of Electroanalytical Chemistry, 646(1-2), 24-32.http://dx.doi.org/10.1016/j.jelechem.2009.11.017
---------- CHICAGO ----------
Flexer, V., Calvo, E.J., Bartlett, P.N. "The application of the relaxation and simplex method to the analysis of data for glucose electrodes based on glucose oxidase immobilised in an osmium redox polymer" . Journal of Electroanalytical Chemistry 646, no. 1-2 (2010) : 24-32.http://dx.doi.org/10.1016/j.jelechem.2009.11.017
---------- MLA ----------
Flexer, V., Calvo, E.J., Bartlett, P.N. "The application of the relaxation and simplex method to the analysis of data for glucose electrodes based on glucose oxidase immobilised in an osmium redox polymer" . Journal of Electroanalytical Chemistry, vol. 646, no. 1-2, 2010, pp. 24-32.http://dx.doi.org/10.1016/j.jelechem.2009.11.017
---------- VANCOUVER ----------
Flexer, V., Calvo, E.J., Bartlett, P.N. The application of the relaxation and simplex method to the analysis of data for glucose electrodes based on glucose oxidase immobilised in an osmium redox polymer. J Electroanal Chem. 2010;646(1-2):24-32.http://dx.doi.org/10.1016/j.jelechem.2009.11.017
