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Abstract:

In the present study a new chromatographic method was developed to quantify melatonin in rat pineal that can be extended to other tissues. Melatonin was extracted from an acid homogenate with ethyl acetate to avoid amine interference. HPLC was performed with silica normal-phase column and fluorescence detection. This method is sensitive enough for detecting melatonin in a single pineal gland with a detection limit of 3 pg/mg tissue.

Registro:

Documento: Artículo
Título:Highly sensitive method for the determination of melatonin by normal-phase high-performance liquid chromatography with fluorometric detection
Autor:Vitale, A.A.; Ferrari, C.C.; Aldana, H.; Affanni, J.M.
Filiación:Proplame-Conicet, Prog. Plantas Toxicas y Medicinales, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Ineuci-Conicet, Instituto de Neurociencias, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Palabras clave:melatonin; melatonin; animal tissue; article; controlled study; drug determination; fluorometry; high performance liquid chromatography; male; nonhuman; pineal body; priority journal; rat; technique
Año:1996
Volumen:681
Número:2
Página de inicio:381
Página de fin:384
DOI: http://dx.doi.org/10.1016/0378-4347(96)00051-5
Título revista:Journal of Chromatography B: Biomedical Applications
Título revista abreviado:J. CHROMATOGR. B BIOMED. APPL.
ISSN:15726495
CODEN:JCBBE
CAS:melatonin, 73-31-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15726495_v681_n2_p381_Vitale

Referencias:

  • Lerner, A.B., Case, J.D., Takahashi, Y., Lee, T.H., Mori, W., (1958) J. Am. Chem. Soc., 80, p. 2587
  • Trentini, G.P., De Gaetani, C., Criscuolo, M., (1991) Aging, 3, p. 103
  • Reiter, R.J., (1992) Bioassays, 14, p. 169
  • Trentini, G.P., Genazzani, A.R., Criscuolo, M., (1992) Neuroendocrinology, 56, p. 364
  • Reiter, R.J., (1993) Braz. J. Med. Biol. Res., 26 (11), p. 1141
  • Pierpaoli, W., Regelson, W., (1994) Proc. Natl. Acad. Sci. USA, 91, p. 787
  • Tamarkin, L., Baird, C.J., Almeida, O.F., (1985) Science, 227, p. 714
  • Chu, E.W., Wurtman, R.J., Axelrod, J., (1964) J. Endocrinol., 75, p. 238
  • Ying, S.Y., Greep, R.O., (1973) Endocrinology, 92, p. 333
  • Menendez-Pelaez, A., Reiter, R.J., (1993) J. Pineal Res., 15, p. 59
  • Huether, G., (1993) Experientia, 49, p. 665
  • Ralph, C.L., Lynch, H.J., (1970) Gen. Comp. Endocrinol., 15, p. 334
  • Miller, F.P., Maickel, R.P., (1970) Life Sci., 9, p. 747
  • Greiner, A.C., Chan, S.S., (1978) Science, 199, p. 83
  • Fuller, R.W., Perry, K.W., (1977) Res. Commun. Pathol. Pharmacol., 18, p. 769
  • Mefford, I.N., Barchas, J.D., (1981) J. Chromatogr., 181, p. 187
  • Anderson, G.M., Young, J.G., Batter, D.K., Young, S.N., Cohen, D.J., Shaywitz, B.A., (1981) J. Chromatogr., 223, p. 315
  • Vakkuri, O., Leppaluoto, J., Vuolteenaho, O., (1984) Acta Endocrinol., 106, p. 152
  • Skene, D.J., Smith, I., Arendt, J., (1986) J. Endocrinol., 110, p. 177
  • Yamada, K.Y., Sakamoto, Y., Satoh, T., (1984) Res. Commun. Chem. Pathol. Pharmacol., 46, p. 283
  • Mills, M.H., King, M.G., (1986) J. Chromatogr., 377, p. 350
  • Anderson, G.M., Young, J.G., Cohen, D.J., (1982) J. Chromatogr., 228, p. 155
  • Wakabayashi, H., Shimada, K., (1986) J. Chromatogr., 381, p. 21
  • Chin, J.L., (1988) J. Chromatogr., 428, p. 206
  • Goldman, M.E., Hamm, H., Erickson, C.K., (1980) J. Chromatogr., 190, p. 217
  • Murai, S., Saito, H., Masuda, Y., Itoh, T., (1988) J. Neurochem., 50, p. 473
  • Hernandez, G., Abreu, P., Alonso, R., Calzadilla, C.H., (1990) J. Pineal Res., 8, p. 11
  • Fourtillan, J.B., Gobin, P., Faye, B., (1994) Biol. Mass Spectrom., 23, p. 499

Citas:

---------- APA ----------
Vitale, A.A., Ferrari, C.C., Aldana, H. & Affanni, J.M. (1996) . Highly sensitive method for the determination of melatonin by normal-phase high-performance liquid chromatography with fluorometric detection. Journal of Chromatography B: Biomedical Applications, 681(2), 381-384.
http://dx.doi.org/10.1016/0378-4347(96)00051-5
---------- CHICAGO ----------
Vitale, A.A., Ferrari, C.C., Aldana, H., Affanni, J.M. "Highly sensitive method for the determination of melatonin by normal-phase high-performance liquid chromatography with fluorometric detection" . Journal of Chromatography B: Biomedical Applications 681, no. 2 (1996) : 381-384.
http://dx.doi.org/10.1016/0378-4347(96)00051-5
---------- MLA ----------
Vitale, A.A., Ferrari, C.C., Aldana, H., Affanni, J.M. "Highly sensitive method for the determination of melatonin by normal-phase high-performance liquid chromatography with fluorometric detection" . Journal of Chromatography B: Biomedical Applications, vol. 681, no. 2, 1996, pp. 381-384.
http://dx.doi.org/10.1016/0378-4347(96)00051-5
---------- VANCOUVER ----------
Vitale, A.A., Ferrari, C.C., Aldana, H., Affanni, J.M. Highly sensitive method for the determination of melatonin by normal-phase high-performance liquid chromatography with fluorometric detection. J. CHROMATOGR. B BIOMED. APPL. 1996;681(2):381-384.
http://dx.doi.org/10.1016/0378-4347(96)00051-5