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Femia, A.L.; Temprana, C.F.; Santos, J.; Carbajal, M.L.; Amor, M.S.; Grasselli, M.; Del Alonso, S.V. "An arsenic fluorescent compound as a novel probe to study arsenic-binding proteins" (2012) Protein Journal. 31(8):656-666
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Abstract:

Arsenic-binding proteins are under continuous research. Their identification and the elucidation of arsenic/ protein interaction mechanisms are important because the biological effects of these complexes may be related not only to arsenic but also to the arsenic/protein structure. Although many proteins bearing a CXXC motif have been found to bind arsenic in vivo, new tools are necessary to identify new arsenic targets and allow research on protein/ arsenic complexes. In this work, we analyzed the performance of the fluorescent compound APAO-FITC (synthesized from p-aminophenylarsenoxide, APAO, and fluorescein isothiocyanate, FITC) in arsenic/protein binding assays using thioredoxin 1 (Trx) as an arsenic-binding protein model. The Trx-APAO-FITC complex was studied through different spectroscopic techniques involving UV-Vis, fluorescence, atomic absorption, infrared and circular dichroism. Our results show that APAO-FITC binds efficiently and specifically to the Trx binding site, labeling the protein fluorescently, without altering its structure and activity. In summary, we were able to study a protein/ arsenic complex model, using APAO-FITC as a labeling probe. The use of APAO-FITC in the identification of different protein and cell targets, as well as in in vivo biodistribution studies, conformational studies of arsenicbinding proteins, and studies for the design of drug delivery systems for arsenic anti-cancer therapies, is highly promising. © Springer Science+Business Media, LLC 2012.

Registro:

Documento: Artículo
Título:An arsenic fluorescent compound as a novel probe to study arsenic-binding proteins
Autor:Femia, A.L.; Temprana, C.F.; Santos, J.; Carbajal, M.L.; Amor, M.S.; Grasselli, M.; Del Alonso, S.V.
Filiación:Laboratorio de Biomembranas, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal, B1876BXD Buenos Aires, Argentina
Laboratorio de Inmunología y Virología, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal, B1876BXD Buenos Aires, Argentina
Instituto de Bioquímica y Biofísica (IQUIFIB), Departamento de Química Biológica, Universidad de Buenos Aires, Buenos Aires, Argentina
LaMaBio, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal, B1876BXD Buenos Aires, Argentina
Palabras clave:Arsenic carcinogenesis; Circular dichroism; Conformational studies; Drug delivery systems; Thioredoxin; 4 aminophenylarsine oxide; arsenic; arsenic binding protein; binding protein; fluorescein isothiocyanate; fluorescent dye; thioredoxin 1; unclassified drug; article; atomic absorption; circular dichroism; enzyme activity; fluorescence; polyacrylamide gel electrophoresis; protein folding; protein interaction; protein structure; Arsenic; Arsenicals; Carrier Proteins; Fluorescein-5-isothiocyanate; Fluorescent Dyes; Kinetics; Models, Molecular; Protein Binding; Protein Conformation; Protein Unfolding; Spectrum Analysis; Temperature; Thioredoxins
Año:2012
Volumen:31
Número:8
Página de inicio:656
Página de fin:666
DOI: http://dx.doi.org/10.1007/s10930-012-9441-6
Título revista:Protein Journal
Título revista abreviado:Protein J.
ISSN:15723887
CODEN:PJROA
CAS:arsenic, 7440-38-2; fluorescein isothiocyanate, 25168-13-2, 27072-45-3, 3326-32-7; Arsenic, 7440-38-2; Arsenicals; Carrier Proteins; Fluorescein-5-isothiocyanate, 3326-32-7; Fluorescent Dyes; Thioredoxins, 52500-60-4; p-aminophenylarsine oxide
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15723887_v31_n8_p656_Femia

Referencias:

  • Ado, K., Taniguchi, Y., (2007) Biochim Biophys Acta, 1774 (7), pp. 813-821
  • Ansorge, W., Sproat, B., Stegemann, J., Schwager, C., Zenke, M., (1987) Nucleic Acids Res, 15 (11), pp. 4593-4602
  • Ansorge, W., Rosenthal, A., Sproat, B., Schwager, C., Stegemann, J., Voss, H., (1988) Nucleic Acids Res, 16 (5), pp. 2203-2206
  • Berry, F.B., Skarie, J.M., Mirzayans, F., Fortin, Y., Hudson, T.J., Raymond, V., Link, B.A., Walter, M.A., (2008) Hum Mol Genet, 17 (4), pp. 490-505
  • Brown, C.J., Dastidar, S.G., See, H.Y., Coomber, D.W., Ortiz-Lombardia, M., Verma, C., Lane, D.P., (2010) J Mol Biol, 395 (4), pp. 871-883
  • Burwell, L.S., Nadtochiy, S.M., Tompkins, A.J., Young, S., Brookes, P.S., (2006) Biochem J, 394 (PART 3), pp. 627-634
  • Carbajal, M.L., Espinoza, S.L.S., Valdez, S.N., Poskus, E., Smolko, E.E., Grasselli, M., (2009) React Funct Polym, 69 (11), pp. 816-820
  • Cleland, W.W., (1964) Biochemistry, 3, pp. 480-482
  • Clerico, E.M., Ermacora, M.R., (2001) Arch Biochem Biophys, 395 (2), pp. 215-224
  • Cline, D.J., Thorpe, C., Schneider, J.P., (2003) J Am Chem Soc, 125 (10), pp. 2923-2929
  • Collet, J.F., Messens, J., (2010) Antioxid Redox Signal, 13 (8), pp. 1205-1216
  • Coto-Garcia, A.M., Sotelo-Gonzalez, E., Fernandez-Arguelles, M.T., Pereiro, R., Costa-Fernandez, J.M., Sanz-Medel, A., (2011) Anal Bioanal Chem, 399 (1), pp. 29-42
  • Chen, Y., Parr, T., Holmes, A.E., Nakanishi, K., (2008) Bioconjug Chem, 19 (1), pp. 5-9
  • Donoghue, N., Yam, P.T., Jiang, X.M., Hogg, P.J., (2000) Protein Sci, 9 (12), pp. 2436-2445
  • Dorward, A., Sweet, S., Moorehead, R., Singh, G., (1997) J Bioenerg Biomembr, 29 (4), pp. 385-392
  • Edgington, L.E., Berger, A.B., Blum, G., Albrow, V.E., Paulick, M.G., Lineberry, N., Bogyo, M., (2009) Nat Med, 15 (8), pp. 967-973
  • Femia, A.L., Temprana, C.F., Amor, M.S., Grasselli, M., Alonso Sdel, V., (2012) Med Chem, 8 (2), pp. 222-229
  • Fernando, M.R., Nanri, H., Yoshitake, S., Nagata-Kuno, K., Minakami, S., (1992) Eur J Biochem/FEBS, 209 (3), pp. 917-922
  • Flora, S.J., (2011) Free Radic Biol Med, 51 (2), pp. 257-281
  • Fu, G.H., Wang, Y., Xi, Y.H., Guo, Z.W., Liu, X.B., Bai, S.Z., Yang, B.F., Chen, G.Q., (2005) J Drug Target, 13 (4), pp. 235-243
  • Ge, Y., Qi, Z., Wang, Y., Liu, X., Li, J., Xu, J., Liu, J., Shen, J., (2009) Int J Biochem Cell Biol, 41 (4), pp. 900-906
  • Gholivand, M.B., Ghasemi, J.B., Saaidpour, S., Mohajeri, A., (2008) Spectrochim Acta A Mol Biomol Spectrosc, 71 (3), pp. 1158-1165
  • Gorman, J.J., (1987) Anal Biochem, 160 (2), pp. 376-387
  • Griffin, B.A., Adams, S.R., Jones, J., Tsien, R.Y., (2000) Methods Enzymol, 327, pp. 565-578
  • Gupta, S., Yel, L., Kim, D., Kim, C., Chiplunkar, S., Gollapudi, S., (2003) Mol Cancer Ther, 2 (8), pp. 711-719
  • Holmgren, A., (1979) J Biol Chem, 254 (19), pp. 9627-9632
  • Holmgren, A., (1995) Structure, 3 (3), pp. 239-243
  • Jeng, M.F., Campbell, A.P., Begley, T., Holmgren, A., Case, D.A., Wright, P.E., Dyson, H.J., (1994) Structure, 2 (9), pp. 853-868
  • Kitchin, K.T., Wallace, K., (2006) J Biochem Mol Toxicol, 20 (1), pp. 48-56
  • Kitchin, K.T., Wallace, K., (2008) J Inorg Biochem, 102 (3), pp. 532-539
  • Laurent, T.C., Moore, E.C., Reichard, P., (1964) J Biol Chem, 239, pp. 3436-3444
  • Layton, C.J., Hellinga, H.W., (2010) Biochemistry, 49 (51), pp. 10831-10841
  • Lundstrom, J., Krause, G., Holmgren, A., (1992) J Biol Chem, 267 (13), pp. 9047-9052
  • Martin, S.R., Schilstra, M.J., (2008) Methods Cell Biol, 84, pp. 263-293
  • Miller Jr., W.H., Schipper, H.M., Lee, J.S., Singer, J., Waxman, S., (2002) Cancer Res, 62 (14), pp. 3893-3903
  • Mitsui, A., Hirakawa, T., Yodoi, J., (1992) Biochem Biophys Res Commun, 186 (3), pp. 1220-1226
  • Nakamura, H., Masutani, H., Yodoi, J., (2006) Semin Cancer Biol, 16 (6), pp. 444-451
  • Nakamura, H., Hoshino, Y., Okuyama, H., Matsuo, Y., Yodoi, J., (2009) Adv Drug Deliv Rev, 61 (4), pp. 303-309
  • Oblong, J.E., Berggren, M., Powis, G., (1994) FEBS Lett, 343 (1), pp. 81-84
  • Olson, E.S., Jiang, T., Aguilera, T.A., Nguyen, Q.T., Ellies, L.G., Scadeng, M., Tsien, R.Y., (2010) Proc Natl Acad Sci USA, pp. 1-6
  • Palaniappan, P.R., Vijayasundaram, V., (2008) Food Chem Toxicol, 46 (11), pp. 3534-3539
  • Ralph, S.J., (2008) Metal Based Drugs, 2008, p. 260146
  • Ramadan, D., Cline, D.J., Bai, S., Thorpe, C., Schneider, J.P., (2007) J Am Chem Soc, 129 (10), pp. 2981-2988
  • Ramadan, D., Rancy, P.C., Nagarkar, R.P., Schneider, J.P., Thorpe, C., (2009) Biochemistry, 48 (2), pp. 424-432
  • Santos, J., Marino-Buslje, C., Kleinman, C., Ermacora, M.R., Delfino, J.M., (2007) Biochemistry, 46 (17), pp. 5148-5159
  • Savickiene, J., Treigyte, G., Gineitis, A., Navakauskiene, R., (2010) Vitro Cell Dev Biol Anim, 46 (6), pp. 547-559
  • Sebastia, J., Cristofol, R., Martin, M., Rodriguez-Farre, E., Sanfeliu, C., (2003) Cytometry A J Int Soc Anal Cytol, 51 (1), pp. 16-25
  • Singh, B.R., Infrared analysis of peptides and proteins: Principles and applications (2000) ACS Symposium Series, 750. , American Chemical Society, Washington
  • Stevenson, K.J., Hale, G., Perham, R.N., (1978) Biochemistry, 17 (11), pp. 2189-2192
  • Stroffekova, K., Proenza, C., Beam, K.G., (2001) Pflugers Archiv Eur J Physiol, 442 (6), pp. 859-866
  • Tseng, C.H., (2004) Toxicol Appl Pharmacol, 197 (2), pp. 67-83
  • Vizioli, N.M., Rusell, M.L., Carbajal, M.L., Carducci, C.N., Grasselli, M., (2005) Electrophoresis, 26 (15), pp. 2942-2948
  • Yeh, A.P., McMillan, A., Stowell, M.H., Acta crystallographica section d (2006) Biol Crystallogr, 62 (PART 4), pp. 451-457
  • Zhang, X., Yang, F., Shim, J.Y., Kirk, K.L., Anderson, D.E., Chen, X., (2007) Cancer Lett, 255 (1), pp. 95-106

Citas:

---------- APA ----------
Femia, A.L., Temprana, C.F., Santos, J., Carbajal, M.L., Amor, M.S., Grasselli, M. & Del Alonso, S.V. (2012) . An arsenic fluorescent compound as a novel probe to study arsenic-binding proteins. Protein Journal, 31(8), 656-666.
http://dx.doi.org/10.1007/s10930-012-9441-6
---------- CHICAGO ----------
Femia, A.L., Temprana, C.F., Santos, J., Carbajal, M.L., Amor, M.S., Grasselli, M., et al. "An arsenic fluorescent compound as a novel probe to study arsenic-binding proteins" . Protein Journal 31, no. 8 (2012) : 656-666.
http://dx.doi.org/10.1007/s10930-012-9441-6
---------- MLA ----------
Femia, A.L., Temprana, C.F., Santos, J., Carbajal, M.L., Amor, M.S., Grasselli, M., et al. "An arsenic fluorescent compound as a novel probe to study arsenic-binding proteins" . Protein Journal, vol. 31, no. 8, 2012, pp. 656-666.
http://dx.doi.org/10.1007/s10930-012-9441-6
---------- VANCOUVER ----------
Femia, A.L., Temprana, C.F., Santos, J., Carbajal, M.L., Amor, M.S., Grasselli, M., et al. An arsenic fluorescent compound as a novel probe to study arsenic-binding proteins. Protein J. 2012;31(8):656-666.
http://dx.doi.org/10.1007/s10930-012-9441-6