Artículo

Celej, M.S.; Dassie, S.A.; Freire, E.; Bianconi, M.L.; Fidelio, G.D. "Ligand-induced thermostability in proteins: Thermodynamic analysis of ANS-albumin interaction" (2005) Biochimica et Biophysica Acta - Proteins and Proteomics. 1750(2):122-133
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Abstract:

A comparative thermodynamic study of the interaction of anilinonaphthalene sulfonate (ANS) derivatives with bovine serum albumin (BSA) was performed by using differential scanning calorimetry (DSC) and isothermal titration calorimetry (ITC). The chemically related ligands, 1,8-ANS and 2,6-ANS, present a similar affinity for BSA with different binding energetics. The analysis of the binding driving forces suggests that not only hydrophobic effect but also electrostatic interactions are relevant, even though they have been extensively used as probes for non-polar domains in proteins. Ligand association leads to an increase in protein thermostability, indicating that both dyes interact mainly with native BSA. ITC data show that 1,8-ANS and 2,6-ANS have a moderate affinity for BSA, with an association constant of around 1-9 × 105 M-1 for the high-affinity site. Ligand binding is disfavoured by conformational entropy. The theoretical model used to simulate DSC data satisfactorily reproduces experimental thermograms, validating this approach as one which provides new insights into the interaction between one or more ligands with a protein. By comparison with 1,8-ANS, 2,6-ANS appears as a more "inert" probe to assess processes which involve conformational changes in proteins. © 2005 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Ligand-induced thermostability in proteins: Thermodynamic analysis of ANS-albumin interaction
Autor:Celej, M.S.; Dassie, S.A.; Freire, E.; Bianconi, M.L.; Fidelio, G.D.
Filiación:Departamento de Química Biológica-CIQUIBIC, Facultad de Ciencias Químicas, Ciudad Universitaria, 5000, Córdoba, Argentina
Unidad de Matemática Y Física-INFIQC, Departamento de Fisicoquímica-INFIQC, Universidad Nacional de Córdoba, Córdoba, Argentina
Instituto Leloir, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Programa de Bioquímica e Biofísica Celular, Instituto de Bioquímica Médica, Universidade Federal Do Rio de Janeiro, Rio de Janeiro, Brazil
Palabras clave:ANS derivative; Linked equilibria; Protein stability; Protein unfolding thermodynamics; Protein-ligand interaction; 8 anilino 1 naphthalenesulfonic acid; albumin; article; binding affinity; differential scanning calorimetry; electricity; energy transfer; entropy; force; hydrophobicity; isothermal titration calorimetry; mathematical computing; priority journal; protein conformation; protein domain; protein interaction; simulation; thermodynamics; thermography; thermostability; validation process; Anilino Naphthalenesulfonates; Animals; Calorimetry, Differential Scanning; Cattle; Heat; Kinetics; Ligands; Models, Molecular; Protein Binding; Protein Denaturation; Protein Folding; Serum Albumin, Bovine; Thermodynamics; Bovinae
Año:2005
Volumen:1750
Número:2
Página de inicio:122
Página de fin:133
DOI: http://dx.doi.org/10.1016/j.bbapap.2005.05.003
Título revista:Biochimica et Biophysica Acta - Proteins and Proteomics
Título revista abreviado:Biochim. Biophys. Acta Proteins Proteomics
ISSN:15709639
CODEN:BBAPB
CAS:8 anilino 1 naphthalenesulfonic acid, 82-76-8; Anilino Naphthalenesulfonates; Ligands; Serum Albumin, Bovine
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15709639_v1750_n2_p122_Celej

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Citas:

---------- APA ----------
Celej, M.S., Dassie, S.A., Freire, E., Bianconi, M.L. & Fidelio, G.D. (2005) . Ligand-induced thermostability in proteins: Thermodynamic analysis of ANS-albumin interaction. Biochimica et Biophysica Acta - Proteins and Proteomics, 1750(2), 122-133.
http://dx.doi.org/10.1016/j.bbapap.2005.05.003
---------- CHICAGO ----------
Celej, M.S., Dassie, S.A., Freire, E., Bianconi, M.L., Fidelio, G.D. "Ligand-induced thermostability in proteins: Thermodynamic analysis of ANS-albumin interaction" . Biochimica et Biophysica Acta - Proteins and Proteomics 1750, no. 2 (2005) : 122-133.
http://dx.doi.org/10.1016/j.bbapap.2005.05.003
---------- MLA ----------
Celej, M.S., Dassie, S.A., Freire, E., Bianconi, M.L., Fidelio, G.D. "Ligand-induced thermostability in proteins: Thermodynamic analysis of ANS-albumin interaction" . Biochimica et Biophysica Acta - Proteins and Proteomics, vol. 1750, no. 2, 2005, pp. 122-133.
http://dx.doi.org/10.1016/j.bbapap.2005.05.003
---------- VANCOUVER ----------
Celej, M.S., Dassie, S.A., Freire, E., Bianconi, M.L., Fidelio, G.D. Ligand-induced thermostability in proteins: Thermodynamic analysis of ANS-albumin interaction. Biochim. Biophys. Acta Proteins Proteomics. 2005;1750(2):122-133.
http://dx.doi.org/10.1016/j.bbapap.2005.05.003