Abstract:
Protein kinase A (PKA) activity was measured in situ in permeabilised Saccharomyces cerevisiae cells in the absence and the presence of cAMP. Four strains genetically predicted to have differential PKA-dependent phenotypes were used: a wild-type strain and a strain containing a bcy1-14 mutation (with almost constitutively active PKA), and the same strains with overexpression of the wild-type or mutant BCY1 gene, respectively. Cells were grown on galactose or glucose. The measured phenotypic characteristics were: trehalose and glycogen levels and the activity of a reporter gene under control of the NTH1 promoter. The 'endogenous' PKA activity (measured in situ in the absence of cAMP) showed the best correlation with the PKA-dependent phenotypes determined in vivo. We propose that this parameter offers a good estimate for the degree of activation of PKA in vivo. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Registro:
Documento: |
Artículo
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Título: | Activation state of protein kinase A as measured in permeabilised Saccharomyces cerevisiae cells correlates with PKA-controlled phenotypes in vivo |
Autor: | Portela, P.; Van Dijck, P.; Thevelein, J.M.; Moreno, S. |
Filiación: | Depto. de Quím. Biol., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina Lab. voor Moleculaire Celbiologie, Vlaams Instituut voor Biotechnologie, Katholieke Universiteit Leuven, Kasteelpark Arenberg 31, 3001 Leuven-Heverlee, Belgium
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Palabras clave: | cAMP; Permeabilisation assay; PKA-dependent phenotype; Protein kinase A; Yeast; cyclic AMP; cyclic AMP dependent protein kinase; galactose; glucose; glycogen; protein; protein BCY1; trehalose; unclassified drug; article; cell growth; cell permeabilization; controlled study; correlation analysis; enzyme activation; enzyme activity; enzyme assay; fungal cell; fungal strain; gene activity; gene expression; gene mutation; genotype; in vivo study; nonhuman; phenotype; promoter region; reporter gene; Saccharomyces cerevisiae; Cyclic AMP; Cyclic AMP-Dependent Protein Kinases; Enzyme Activation; Galactose; Genes, Reporter; Glucose; Mutation; Phenotype; Saccharomyces cerevisiae; Substrate Specificity; Saccharomyces cerevisiae |
Año: | 2003
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Volumen: | 3
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Número: | 1
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Página de inicio: | 119
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Página de fin: | 126
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DOI: |
http://dx.doi.org/10.1016/S1567-1356(02)00158-7 |
Título revista: | FEMS Yeast Research
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Título revista abreviado: | FEMS Yeast Res.
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ISSN: | 15671356
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CODEN: | FYREA
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CAS: | cyclic AMP, 60-92-4; galactose, 26566-61-0, 50855-33-9, 59-23-4; glucose, 50-99-7, 84778-64-3; glycogen, 9005-79-2; protein, 67254-75-5; trehalose, 99-20-7; Cyclic AMP, 60-92-4; Cyclic AMP-Dependent Protein Kinases, EC 2.7.1.37; Galactose, 26566-61-0; Glucose, 50-99-7
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15671356_v3_n1_p119_Portela |
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Citas:
---------- APA ----------
Portela, P., Van Dijck, P., Thevelein, J.M. & Moreno, S.
(2003)
. Activation state of protein kinase A as measured in permeabilised Saccharomyces cerevisiae cells correlates with PKA-controlled phenotypes in vivo. FEMS Yeast Research, 3(1), 119-126.
http://dx.doi.org/10.1016/S1567-1356(02)00158-7---------- CHICAGO ----------
Portela, P., Van Dijck, P., Thevelein, J.M., Moreno, S.
"Activation state of protein kinase A as measured in permeabilised Saccharomyces cerevisiae cells correlates with PKA-controlled phenotypes in vivo"
. FEMS Yeast Research 3, no. 1
(2003) : 119-126.
http://dx.doi.org/10.1016/S1567-1356(02)00158-7---------- MLA ----------
Portela, P., Van Dijck, P., Thevelein, J.M., Moreno, S.
"Activation state of protein kinase A as measured in permeabilised Saccharomyces cerevisiae cells correlates with PKA-controlled phenotypes in vivo"
. FEMS Yeast Research, vol. 3, no. 1, 2003, pp. 119-126.
http://dx.doi.org/10.1016/S1567-1356(02)00158-7---------- VANCOUVER ----------
Portela, P., Van Dijck, P., Thevelein, J.M., Moreno, S. Activation state of protein kinase A as measured in permeabilised Saccharomyces cerevisiae cells correlates with PKA-controlled phenotypes in vivo. FEMS Yeast Res. 2003;3(1):119-126.
http://dx.doi.org/10.1016/S1567-1356(02)00158-7