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Abstract:

Trypanosoma cruzi, the etiological agent of Chagas disease, was initially classified into 6 Discrete Typing Units (DTUs). The hybrid DTUs TcV and TcVI are the most frequent in domestic transmission cycles throughout the Southern Cone countries of South America. Here, we genotyped parasite isolates from human residents in Pampa del Indio municipality, Chaco, to further characterize the structure of T. cruzi populations, and to assess the degree of overlapping between the domestic and sylvatic transmission cycles. Artificial xenodiagnostic tests were performed to blood samples from 125 T. cruzi-seropositive people (age range, 3–70 years) who represented 14.3% of all seropositive residents identified. Parasites were obtained from feces of T. cruzi-infected Triatoma infestans examined 30 or 60 days after blood-feeding, and grown in vitro. The cultured parasites were genotyped by means of two PCR-based protocols. DTUs were determined from 39 (31%) patients residing in 28 dwellings. The only DTUs identified were TcV (92%) and TcVI (8–36%). Households with more than one parasite isolate consistently displayed the same DTU. Further sequencing of a fragment of the TcMK gene from selected samples argue against the occurrence of mixed TcV-TcVI infections in the study population. Sequencing data revealed an unexpected degree of genetic variability within TcV including two apparently robust subgroups of isolates. Our results for human residents confirm the predominance of hybrid lineages (TcV and to a much lesser extent TcVI) and the absence of sylvatic genotypes (TcI and TcIII) in (peri)domestic transmission cycles in the Argentinean Chaco area. 245 words. © 2018 Elsevier B.V.

Registro:

Documento: Artículo
Título:New human isolates of Trypanosoma cruzi confirm the predominance of hybrid lineages in domestic transmission cycle of the Argentinean Chaco
Autor:Macchiaverna, N.P.; Enriquez, G.F.; Buscaglia, C.A.; Balouz, V.; Gürtler, R.E.; Cardinal, M.V.
Filiación:Laboratorio de Eco-Epidemiología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires e Instituto de Ecología, Genética y Evolución de Buenos Aires (IEGEBA), UBA-CONICET, Capital Federal, Argentina
Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECh), Universidad Nacional de San Martín (UNSAM) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Palabras clave:Chaco; DTU; Human; Isolates; Molecular epidemiology; Trypanosoma cruzi; adolescent; adult; aged; Argentina; Article; child; controlled study; female; human; major clinical study; male; mixed infection; molecular epidemiology; nonhuman; parasite isolation; parasite transmission; polymerase chain reaction; population structure; priority journal; rural area; single nucleotide polymorphism; Triatoma infestans; Trypanosoma cruzi; xenodiagnosis
Año:2018
Volumen:66
Página de inicio:229
Página de fin:235
DOI: http://dx.doi.org/10.1016/j.meegid.2018.10.001
Título revista:Infection, Genetics and Evolution
Título revista abreviado:Infec. Genet. Evol.
ISSN:15671348
CODEN:IGENC
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15671348_v66_n_p229_Macchiaverna

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Citas:

---------- APA ----------
Macchiaverna, N.P., Enriquez, G.F., Buscaglia, C.A., Balouz, V., Gürtler, R.E. & Cardinal, M.V. (2018) . New human isolates of Trypanosoma cruzi confirm the predominance of hybrid lineages in domestic transmission cycle of the Argentinean Chaco. Infection, Genetics and Evolution, 66, 229-235.
http://dx.doi.org/10.1016/j.meegid.2018.10.001
---------- CHICAGO ----------
Macchiaverna, N.P., Enriquez, G.F., Buscaglia, C.A., Balouz, V., Gürtler, R.E., Cardinal, M.V. "New human isolates of Trypanosoma cruzi confirm the predominance of hybrid lineages in domestic transmission cycle of the Argentinean Chaco" . Infection, Genetics and Evolution 66 (2018) : 229-235.
http://dx.doi.org/10.1016/j.meegid.2018.10.001
---------- MLA ----------
Macchiaverna, N.P., Enriquez, G.F., Buscaglia, C.A., Balouz, V., Gürtler, R.E., Cardinal, M.V. "New human isolates of Trypanosoma cruzi confirm the predominance of hybrid lineages in domestic transmission cycle of the Argentinean Chaco" . Infection, Genetics and Evolution, vol. 66, 2018, pp. 229-235.
http://dx.doi.org/10.1016/j.meegid.2018.10.001
---------- VANCOUVER ----------
Macchiaverna, N.P., Enriquez, G.F., Buscaglia, C.A., Balouz, V., Gürtler, R.E., Cardinal, M.V. New human isolates of Trypanosoma cruzi confirm the predominance of hybrid lineages in domestic transmission cycle of the Argentinean Chaco. Infec. Genet. Evol. 2018;66:229-235.
http://dx.doi.org/10.1016/j.meegid.2018.10.001