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Kinkar, L.; Laurimäe, T.; Acosta-Jamett, G.; Andresiuk, V.; Balkaya, I.; Casulli, A.; Gasser, R.B.; González, L.M.; Haag, K.L.; Zait, H.; Irshadullah, M.; Jabbar, A.; Jenkins, D.J.; Manfredi, M.T.; Mirhendi, H.; M'rad, S.; Rostami-Nejad, M.; Oudni-M'rad, M. (...) Saarma, U. "Distinguishing Echinococcus granulosus sensu stricto genotypes G1 and G3 with confidence: A practical guide" (2018) Infection, Genetics and Evolution. 64:178-184
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Abstract:

Cystic echinococcosis (CE), a zoonotic disease caused by tapeworms of the species complex Echinococcus granulosus sensu lato, represents a substantial global health and economic burden. Within this complex, E. granulosus sensu stricto (genotypes G1 and G3) is the most frequent causative agent of human CE. Currently, there is no fully reliable method for assigning samples to genotypes G1 and G3, as the commonly used mitochondrial cox1 and nad1 genes are not sufficiently consistent for the identification and differentiation of these genotypes. Thus, a new genetic assay is required for the accurate assignment of G1 and G3. Here we use a large dataset of near-complete mtDNA sequences (n = 303) to reveal the extent of genetic variation of G1 and G3 on a broad geographical scale and to identify reliable informative positions for G1 and G3. Based on extensive sampling and sequencing data, we developed a new method, that is simple and cost-effective, to designate samples to genotypes G1 and G3. We found that the nad5 is the best gene in mtDNA to differentiate between G1 and G3, and developed new primers for the analysis. Our results also highlight problems related to the commonly used cox1 and nad1. To guarantee consistent identification of G1 and G3, we suggest using the sequencing of the nad5 gene region (680 bp). This region contains six informative positions within a relatively short fragment of the mtDNA, allowing the differentiation of G1 and G3 with confidence. Our method offers clear advantages over the previous ones, providing a significantly more consistent means to distinguish G1 and G3 than the commonly used cox1 and nad1. © 2018 Elsevier B.V.

Registro:

Documento: Artículo
Título:Distinguishing Echinococcus granulosus sensu stricto genotypes G1 and G3 with confidence: A practical guide
Autor:Kinkar, L.; Laurimäe, T.; Acosta-Jamett, G.; Andresiuk, V.; Balkaya, I.; Casulli, A.; Gasser, R.B.; González, L.M.; Haag, K.L.; Zait, H.; Irshadullah, M.; Jabbar, A.; Jenkins, D.J.; Manfredi, M.T.; Mirhendi, H.; M'rad, S.; Rostami-Nejad, M.; Oudni-M'rad, M.; Pierangeli, N.B.; Ponce-Gordo, F.; Rehbein, S.; Sharbatkhori, M.; Kia, E.B.; Simsek, S.; Soriano, S.V.; Sprong, H.; Šnábel, V.; Umhang, G.; Varcasia, A.; Saarma, U.
Filiación:Department of Zoology, Institute of Ecology and Earth Sciences, University of Tartu, Vanemuise 46, Tartu, 51003, Estonia
Instituto de Medicina Preventiva Veterinaria y Programa de Investigación Aplicada en Fauna Silvestre, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile
Laboratorio de Zoonosis Parasitarias, FCEyN, UNMdP, Funes 3350, CP: 7600 Mar del Plata, Buenos Aires, Argentina
Department of Parasitology, Faculty of Veterinary Medicine, University of Atatürk, Erzurum, Turkey
World Health Organization Collaborating Centre for the Epidemiology, Detection and Control of Cystic and Alveolar Echinococcosis, European Union Reference Laboratory for Parasites (EURLP), Istituto Superiore di Sanità, Viale Regina Elena 299, Rome, 00161, Italy
Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia
Parasitology Department, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Majadahonda, Madrid, 28220, Spain
Departamento de Genética, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, Porto Alegre, RS, Brazil
Parasitology and Mycology department, Mustapha University Hospital, Algiers, 16000, Algeria
Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh, 202002, India
School of Animal and Veterinary Sciences, Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW 2678, Australia
Department of Veterinary Medicine, Università degli Studi di Milano, via Celoria 10, Milan, 20133, Italy
Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Laboratory of Medical and Molecular Parasitology-Mycology (LP3M), LR 12ES08, Faculty of Pharmacy, University of Monastir, Monastir, 5000, Tunisia
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Department of Microbiology and Parasitology, Faculty of Medical Sciences, Comahue National University, 1400, NeuquénBuenos Aires 8300, Argentina
Department of Parasitology, Faculty of Pharmacy, Complutense University, Plaza Ramón y Cajal s/n, Madrid, 28040, Spain
Merial GmbH, Kathrinenhof Research Center, Walchenseestr. 8–12, Rohrdorf, 83101, Germany
Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, Elazig, 23119, Turkey
Centre for Infectious Disease Control Netherlands, National Institute for Public Health and Environment, P.O. Box 1, Bilthoven, BA 3720, Netherlands
Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, Košice, Košice 040 01, Slovakia
ANSES, Nancy Laboratory for Rabies and Wildlife, Wildlife surveillance and eco-epidemiology unit, Malzéville, 54220, France
Laboratory of Parasitology, Veterinary Teaching Hospital, Department of Veterinary Medicine, University of Sassari, Via Vienna 2, Sassari, 07100, Italy
Palabras clave:Cystic echinococcosis; Echinococcus granulosus sensu stricto; Genotype identification; Mitochondrial markers; The nad5 gene; mitochondrial DNA; Article; controlled study; cox1 gene; DNA sequence; Echinococcus granulosus; Echinococcus granulosus genotype 1; Echinococcus granulosus genotype 3; genetic variation; mitochondrial gene; nad1 gene; nad5 gene; nonhuman; priority journal
Año:2018
Volumen:64
Página de inicio:178
Página de fin:184
DOI: http://dx.doi.org/10.1016/j.meegid.2018.06.026
Título revista:Infection, Genetics and Evolution
Título revista abreviado:Infec. Genet. Evol.
ISSN:15671348
CODEN:IGENC
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15671348_v64_n_p178_Kinkar

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Citas:

---------- APA ----------
Kinkar, L., Laurimäe, T., Acosta-Jamett, G., Andresiuk, V., Balkaya, I., Casulli, A., Gasser, R.B.,..., Saarma, U. (2018) . Distinguishing Echinococcus granulosus sensu stricto genotypes G1 and G3 with confidence: A practical guide. Infection, Genetics and Evolution, 64, 178-184.
http://dx.doi.org/10.1016/j.meegid.2018.06.026
---------- CHICAGO ----------
Kinkar, L., Laurimäe, T., Acosta-Jamett, G., Andresiuk, V., Balkaya, I., Casulli, A., et al. "Distinguishing Echinococcus granulosus sensu stricto genotypes G1 and G3 with confidence: A practical guide" . Infection, Genetics and Evolution 64 (2018) : 178-184.
http://dx.doi.org/10.1016/j.meegid.2018.06.026
---------- MLA ----------
Kinkar, L., Laurimäe, T., Acosta-Jamett, G., Andresiuk, V., Balkaya, I., Casulli, A., et al. "Distinguishing Echinococcus granulosus sensu stricto genotypes G1 and G3 with confidence: A practical guide" . Infection, Genetics and Evolution, vol. 64, 2018, pp. 178-184.
http://dx.doi.org/10.1016/j.meegid.2018.06.026
---------- VANCOUVER ----------
Kinkar, L., Laurimäe, T., Acosta-Jamett, G., Andresiuk, V., Balkaya, I., Casulli, A., et al. Distinguishing Echinococcus granulosus sensu stricto genotypes G1 and G3 with confidence: A practical guide. Infec. Genet. Evol. 2018;64:178-184.
http://dx.doi.org/10.1016/j.meegid.2018.06.026