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Abstract:

The patterns of genetic structure in natural populations provide essential information for the improvement of pest management strategies including those targeting arthropod vectors of human diseases. We analyzed the patterns of fine-scale genetic structure in Triatoma infestans in a well-defined rural area close to Pampa del Indio, in the Argentine Arid-Humid Chaco transition, where a longitudinal study on house infestation and wing geometric morphometry is being conducted since 2007. A total of 228 insects collected in 16 domestic and peridomestic sites from two rural communities was genotyped for 10 microsatellite loci and analyzed. We did not find departures from Hardy-Weinberg expectations in collection sites, with three exceptions probably due to null alleles and substructuring. Domestic sites were more variable than peridomestic sites suggesting the presence of older bug populations in domestic sites or higher effective population sizes. Significant genetic structure was detected using F-statistics, a discriminant analysis of principal components and Bayesian clustering algorithms in an area of only 6.32km2. Microsatellite markers detected population structuring at a finer geographic scale (180-6300m) than a previous study based on wing geometric morphometry (>4000m). The spatial distribution of genetic variability was more properly explained by a hierarchical island than by an isolation-by-distance model. This study illustrates that, despite more than a decade without vector control interventions enhancing differentiation, genetic structure can be detected in T. infestans populations, particularly applying spatial information. This supports the potential of genetic studies to provide key information for hypothesis testing of the origins of house reinfestation. © 2015 Elsevier B.V.

Registro:

Documento: Artículo
Título:Fine-scale genetic structure of Triatoma infestans in the Argentine Chaco
Autor:Piccinali, R.V.; Gürtler, R.E.
Filiación:Laboratorio de Eco-Epidemiologia, Departamento de Ecologia, Genética y Evolución, Instituto de Ecología, Genética y Evolución de Buenos Aires (CONICET-UBA), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon 2, Ciudad Universitaria, Int. Guiraldes 2160, Ciudad Autónoma de Buenos Aires, C1429EGA, Argentina
Palabras clave:Genetic structure; Gran Chaco; Microsatellites; Triatoma infestans; Argentina; Article; controlled study; domestic animal; effective population size; gene cluster; gene linkage disequilibrium; genotype; longitudinal study; microsatellite marker; morphometrics; nonhuman; null allele; pest infestation; population genetic structure; priority journal; rural area; Triatoma infestans; vector control; animal; Chagas disease; disease carrier; DNA sequence; ecosystem; female; gene; gene frequency; genetics; human; insect control; male; transmission; Triatoma; Arthropoda; Hexapoda; Triatoma infestans; microsatellite DNA; Animals; Chagas Disease; Ecosystem; Female; Gene Frequency; Genes, Insect; Genotype; Humans; Insect Control; Insect Vectors; Linkage Disequilibrium; Longitudinal Studies; Male; Microsatellite Repeats; Sequence Analysis, DNA; Triatoma
Año:2015
Volumen:34
Página de inicio:143
Página de fin:152
DOI: http://dx.doi.org/10.1016/j.meegid.2015.05.030
Título revista:Infection, Genetics and Evolution
Título revista abreviado:Infec. Genet. Evol.
ISSN:15671348
CODEN:IGENC
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15671348_v34_n_p143_Piccinali

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Citas:

---------- APA ----------
Piccinali, R.V. & Gürtler, R.E. (2015) . Fine-scale genetic structure of Triatoma infestans in the Argentine Chaco. Infection, Genetics and Evolution, 34, 143-152.
http://dx.doi.org/10.1016/j.meegid.2015.05.030
---------- CHICAGO ----------
Piccinali, R.V., Gürtler, R.E. "Fine-scale genetic structure of Triatoma infestans in the Argentine Chaco" . Infection, Genetics and Evolution 34 (2015) : 143-152.
http://dx.doi.org/10.1016/j.meegid.2015.05.030
---------- MLA ----------
Piccinali, R.V., Gürtler, R.E. "Fine-scale genetic structure of Triatoma infestans in the Argentine Chaco" . Infection, Genetics and Evolution, vol. 34, 2015, pp. 143-152.
http://dx.doi.org/10.1016/j.meegid.2015.05.030
---------- VANCOUVER ----------
Piccinali, R.V., Gürtler, R.E. Fine-scale genetic structure of Triatoma infestans in the Argentine Chaco. Infec. Genet. Evol. 2015;34:143-152.
http://dx.doi.org/10.1016/j.meegid.2015.05.030