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Abstract:

The recent discovery of sylvatic populations of Triatoma infestans outside the Andean Valleys of Bolivia prompted an evolutionary question about the putative ancestral area of origin and dispersal of the species, and an epidemiological question regarding the possible role of these sylvatic populations in the recolonization process of insecticide-treated houses. The finding of a population of sylvatic melanic T. infestans (dark morphs) in the Argentinean dry Chaco at 7. km from a peridomestic bug population of typical coloration gave us the opportunity to test both questions simultaneously by employing phylogenetic and population genetic approaches. For this purpose we analyzed sylvatic and peridomestic bugs using sequence-based mitochondrial and nuclear markers (mtCOI and ITS-1) and microsatellites. Sylvatic bugs were confirmed to be T. infestans and not hybrids, and showed high levels of genetic variability and departures from neutral expectations for mtCOI variation. New ITS-1 and mtCOI haplotypes were recorded, as well as haplotypes shared with peridomestic and/or domestic bugs from previous records. The peridomestic population was invariant for ITS-1 and mtCOI, but showed variability for microsatellites and signatures of a population bottleneck, probably due to a limited number of founders. Phylogenetic analyses were consistent with the presence of ancestral haplotypes in sylvatic bugs. According to F-statistics and assignment methods there was a significant differentiation between sylvatic and peridomestic bugs and gene flow was low and asymmetric, with more bugs moving from the peridomicile to the sylvatic environment. These results support the hypothesis of the Chaco region as the area of origin of T. infestans, and a limited role of sylvatic melanic T. infestans in peridomestic infestation in the Argentinean Chaco. © 2011 Elsevier B.V.

Registro:

Documento: Artículo
Título:Genetic variability, phylogenetic relationships and gene flow in Triatoma infestans dark morphs from the Argentinean Chaco
Autor:Piccinali, R.V.; Marcet, P.L.; Ceballos, L.A.; Kitron, U.; Gürtler, R.E.; Dotson, E.M.
Filiación:Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina
Centers for Disease Control and Prevention, Division of Parasitic Diseases and Malaria, Entomology Branch, Atlanta, GA, United States
Department of Environmental Studies, Emory University, Atlanta, GA, United States
Palabras clave:Dark morphs; Epidemiological relevance; Evolutionary history; Gene flow; Triatoma infestans; mitochondrial protein; nuclear protein; protein ITS 1; protein mtCOI; unclassified drug; article; bottleneck population; controlled study; domestic species; environmental factor; gene; gene flow; gene sequence; genetic variability; haplotype; insect genetics; ITS 1 gene; microsatellite marker; mtCOI gene; nonhuman; nucleotide sequence; phylogeny; population genetics; priority journal; Triatoma infestans; Animals; Argentina; Base Sequence; DNA, Intergenic; Electron Transport Complex IV; Gene Expression Regulation; Gene Flow; Genetic Variation; Haplotypes; Mitochondria; Phylogeny; Pigments, Biological; Triatoma; Triatoma infestans
Año:2011
Volumen:11
Número:5
Página de inicio:895
Página de fin:903
DOI: http://dx.doi.org/10.1016/j.meegid.2011.02.013
Título revista:Infection, Genetics and Evolution
Título revista abreviado:Infec. Genet. Evol.
ISSN:15671348
CODEN:IGENC
CAS:DNA, Intergenic; Electron Transport Complex IV, 1.9.3.1; Pigments, Biological
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15671348_v11_n5_p895_Piccinali

Referencias:

  • Bacigalupo, A., Segura, J.A., Garcia, A., Hidalgo, J., Galuppo, S., Cattan, P.E., First finding of Chagas disease vectors associated with wild bushes in the Metropolitan Region of Chile (2006) Rev. Med. Chil., 134 (10), pp. 1230-1236
  • Bacigalupo, A., Torres-Perez, F., Segovia, V., Garcia, A., Correa, J.P., Moreno, L., Arroyo, P., Cattan, P.E., Sylvatic foci of the Chagas disease vector Triatoma infestans in Chile: description of a new focus and challenges for control programs (2010) Mem. Inst. Oswaldo Cruz, 105 (5), pp. 633-641
  • Bargues, M.D., Klisiowicz, D.R., Panzera, F., Noireau, F., Marcilla, A., Perez, R., Rojas, M.G., Mas-Coma, S., Origin and phylogeography of the Chagas disease main vector Triatoma infestans based on nuclear rDNA sequences and genome size (2006) Infect. Genet. Evol., 6 (1), pp. 46-62
  • Buitrago, R., Waleckx, E., Bosseno, M.F., Zoveda, F., Vidaurre, P., Salas, R., Mamani, E., Breniere, S.F., First report of widespread wild populations of Triatoma infestans (Reduviidae, Triatominae) in the valleys of La Paz, Bolivia (2010) Am. J. Trop. Med. Hyg., 82 (4), pp. 574-579
  • Carcavallo, R.U., Phylogeny of Triatominae. The Triatoma infestans complex (1998) Mem. Inst. Oswaldo Cruz, 93 (SUPPL. 2), pp. 68-70
  • Carlsson, J., Effects of microsatellite null alleles on assignment testing (2008) J. Hered., 99 (6), pp. 616-623
  • Ceballos, L.A., Ciclo silvestre de transmisión de Trypanosoma cruzi en el noroeste de Argentina (2010) Doctoral Thesis, p. 176. , Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
  • Ceballos, L.A., Piccinali, R.V., Berkunsky, I., Kitron, U., Gürtler, R.E., First finding of melanic sylvatic Triatoma infestans (Hemiptera: Reduviidae) colonies in the Argentine Chaco (2009) J. Med. Entomol., 46 (5), pp. 1195-1202
  • Clement, M., Posada, D., Crandall, K.A., TCS: a computer program to estimate gene genealogies (2000) Mol. Ecol., 9 (10), pp. 1657-1659
  • Cornuet, J.M., Luikart, G., Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data (1996) Genetics, 144 (4), pp. 2001-2014
  • Cortez, M.R., Emperaire, L., Piccinali, R.V., Gurtler, R.E., Torrico, F., Jansen, A.M., Noireau, F., Sylvatic Triatoma infestans (Reduviidae Triatominae) in the Andean valleys of Bolivia (2007) Acta Trop., 102, pp. 47-54
  • Cortez, M.R., Monteiro, F.A., Noireau, F., New insights on the spread of Triatoma infestans from Bolivia-implications for Chagas disease emergence in the southern cone (2010) Infect. Genet. Evol., 10 (2), pp. 350-353
  • Dujardin, J.P., Bermudez, H., Schofield, C.J., The use of morphometrics in entomological surveillance of sylvatic foci of Triatoma infestans in Bolivia (1997) Acta Trop., 66 (3), pp. 145-153
  • Dujardin, J.P., Tibayrenc, M., Venegas, E., Maldonado, L., Desjeux, P., Ayala, F.J., Isozyme evidence of lack of speciation between wild and domestic Triatoma infestans (Heteroptera: Reduviidae) in Bolivia (1987) J. Med. Entomol., 24 (19), pp. 40-45
  • Earl, D.A., (2010) Structure Harvester v0.56.4, , http://taylor0.biology.ucla.edu/struct_harvest, Program available from website:, (last accessed 31.08.10)
  • Evanno, G., Regnaut, S., Goudet, J., Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study (2005) Mol. Ecol., 14 (8), pp. 2611-2620
  • Excoffier, L., Lischer, H.E.L., Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows (2010) Mol. Ecol. Res., 10 (3), pp. 564-567
  • Fu, Y.-X., Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection (1997) Genetics, 147 (2), pp. 915-925
  • Funk, D.J., Omland, K.E., Species-level paraphyly and polyphyly: frequency, causes and consequences, with insights from animal mitochondrial DNA (2003) Annu. Rev. Ecol. Evol. Syst., 34, pp. 397-423
  • Huelsenbeck, J.P., Ronquist, F., MRBAYES: Bayesian inference of phylogenetic trees (2001) Bioinformatics, 17 (8), pp. 754-755
  • Kuhner, M.K., LAMARC 2.0: maximum likelihood and Bayesian estimation of population parameters (2006) Bioinformatics, 22 (6), pp. 768-770
  • Kumar, S., Nei, M., Dudley, J., Tamura, K., MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences (2008) Brief. Bioinform., 9 (4), pp. 299-306
  • Librado, P., Rozas, J., DnaSP v5: a software for comprehensive analysis of DNA polymorphism data (2009) Bioinformatics, 25 (11), pp. 1451-1452
  • Lowe, A., Harris, S., Ashton, P., (2004) Ecological Genetics. Design, Analysis, and Application, , Blackwell Publishing, Malden, MA
  • Marcet, P.L., Estructura genético-poblacional de Triatoma infestans en comunidades rurales del Noroeste Argentino. Doctoral Thesis (2009) Departmento de Ecología, Genética y Evolución, p. 141. , Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
  • Marcet, P.L., Lehmann, T., Groner, G., Gürtler, R.E., Kitron, U., Dotson, E.M., Identification and characterization of microsatellite markers in the Chagas disease vector Triatoma infestans (Heteroptera: Reduviidae) (2006) Infect. Genet. Evol., 6 (1), pp. 32-37
  • Marcet, P.L., Mora, M.S., Cutrera, A.P., Jones, L., Gürtler, R.E., Kitron, U., Dotson, E.M., Genetic structure of Triatoma infestans populations in rural communities of Santiago del Estero, northern Argentina (2008) Infect. Genet. Evol., 8 (6), pp. 835-846
  • Mas-Coma, S., Bargues, M.D., Populations, hybrids and the systematic concepts of species and subspecies in Chagas disease triatomine vectors inferred from nuclear ribosomal and mitochondrial DNA (2009) Acta Trop., 110 (2-3), pp. 112-136
  • Monteiro, F.A., Barrett, T.V., Fitzpatrick, S., Cordon-Rosales, C., Feliciangeli, D., Beard, C.B., Molecular phylogeography of the Amazonian Chagas disease vectors Rhodnius prolixus and R. robustus (2003) Mol. Ecol., 12 (4), pp. 997-1006
  • Monteiro, F.A., Perez, R., Panzera, F., Dujardin, J.P., Galvao, C., Rocha, D., Noireau, F., Beard, C.B., Mitochondrial DNA variation of Triatoma infestans populations and its implication on the specific status of T. melanosoma (1999) Mem. Inst. Oswaldo Cruz, 94 (SUPPL. 1), pp. 229-238
  • Noireau, F., Wild Triatoma infestans, a potential threat that needs to be monitored (2009) Mem. Inst. Oswaldo Cruz, 104 (SUPPL. 1), pp. 60-64
  • Noireau, F., Flores, R., Gutierrez, T., Dujardin, J.P., Detection of sylvatic dark morphs of Triatoma infestans in the Bolivian Chaco (1997) Mem. Inst. Oswaldo Cruz, 92 (59), pp. 583-584
  • Noireau, F., Bastrenta, B., Catala, S., Dujardin, J.P., Panzera, F., Torres, M., Perez, R., Jurberg, J., Sylvatic population of Triatoma infestans from the Bolivian Chaco: from field collection to characterization (2000) Mem. Inst. Oswaldo Cruz, 95 (SUPPL. 1), pp. 119-122
  • Noireau, F., Cortez, M.G., Monteiro, F.A., Jansen, A.M., Torrico, F., Can wild Triatoma infestans foci in Bolivia jeopardize Chagas disease control efforts? (2005) Trends Parasitol., 21 (1), pp. 7-10
  • Nylander, J.A., Wilgenbusch, J.C., Warren, D.L., Swofford, D.L., AWTY (are we there yet?): a system for graphical exploration of MCMC convergence in Bayesian phylogenetics (2008) Bioinformatics, 24 (4), pp. 581-583
  • Nylander, J.A.A., (2004) MrModeltest v2. Program Distributed by the Author, , http://www.abc.se/nylander/mrmodeltest2/mrmodeltest2.html, Evolutionary Biology Centre, Uppsala University, (last accessed 31.08.10)
  • Paetkau, D., Slade, R., Burden, M., Estoup, A., Genetic assignment methods for the direct, real-time estimation of migration rate: a simulation-based exploration of accuracy and power (2004) Mol. Ecol., 13 (1), pp. 55-65
  • Panzera, F., Dujardin, J.P., Nicolini, P., Caraccio, M.N., Rose, V., Tellez, T., Bermudez, H., Perez, R., Genomic changes of Chagas disease vector, South America (2004) Emerg. Infect. Dis., 10 (3), pp. 438-446
  • Pérez, R., Hernández, M., Quintero, O., Scvortzoff, E., Canale, D., Méndez, L., Cohanoff, C., Panzera, F., Cytogenetic analysis of experimental hybrids in species of Triatominae (Hemiptera-Reduviidae) (2005) Genetica, 125 (2), pp. 261-270
  • Piccinali, R.V., Canale, D.M., Sandoval, A.E., Cardinal, M.V., Jensen, O., Kitron, U., Gürtler, R.E., Triatoma infestans bugs in Southern Patagonia, Argentina (2010) Emerg. Infect. Dis., 16 (5), pp. 887-889
  • Piccinali, R.V., Marcet, P.L., Noireau, F., Kitron, U., Gurtler, R.E., Dotson, E.M., Molecular population genetics and phylogeography of the Chagas disease vector Triatoma infestans in South America (2009) J. Med. Entomol., 46 (4), pp. 796-809
  • Piry, S., Alapetite, A., Cornuet, J.M., Paetkau, D., Baudouin, L., Estoup, A., GENECLASS2: a software for genetic assignment and first-generation migrant detection (2004) J. Hered., 95 (6), pp. 536-539
  • Posada, D., Crandall, K.A., Intraspecific gene genealogies: trees grafting into networks (2001) Trends Ecol. Evol., 16 (1), pp. 37-45
  • Pritchard, J.K., Stephens, M., Donnelly, P., Inference of population structure using multilocus genotype data (2000) Genetics, 155 (2), pp. 945-959
  • Rambout, A., Drummond, A.J., (2009) Tracer v1.5. Program Distributed by the Authors, , http://tree.bio.ed.ac.uk/software/tracer/, (last accessed 31.08.10)
  • Ramos-Onsins, S.E., Rozas, J., Statistical properties of new neutrality tests against population growth (2002) Mol. Biol. Evol., 19 (12), pp. 2092-2100
  • Rannala, B., Mountain, J.L., Detecting immigration by using multilocus genotypes (1997) Proc. Natl. Acad. Sci. U. S. A., 94 (17), pp. 9197-9201
  • Rice, W., Analyzing tables of statistical tests (1989) Evolution, 43 (1), pp. 223-225
  • Richer, W., Kengne, P., Cortez, M.R., Perrineau, M.M., Cohuet, A., Fontenille, D., Noireau, F., Active dispersal by wild Triatoma infestans in the Bolivian Andes (2007) Trop. Med. Int. Health, 12 (6), pp. 759-764
  • Rodriguero, M.S., Origen y consecuencias de la reproducción asexual en una especie de gorgojo de importancia agronómica. Doctoral Thesis (2009) Departmento de Ecología, Genética y Evolución, p. 360. , Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
  • Ronquist, F., Huelsenbeck, J.P., MrBayes 3: Bayesian phylogenetic inference under mixed models (2003) Bioinformatics, 19 (12), pp. 1572-1574
  • Rousset, F., Genepop'007: a complete re-implementation of the Genepop software for Windows and Linux (2008) Mol. Ecol. Res., 8 (1), pp. 103-106
  • Schofield, C.J., Biosystematics of the Triatominae (1988) Biosystematics of Haematophagous Insects, pp. 284-312. , Clarendon Press, Oxford, M.W. Service (Ed.)
  • Schofield, C.J., Lehane, M.J., McEwen, P., Catalá, S.S., Gorla, D.E., Dispersive flight by Triatoma infestans under natural climatic conditions in Argentina (1992) Med. Vet. Entomol., 6 (1), pp. 51-56
  • Schlötterer, C., Evolutionary dynamics of microsatellite DNA (2000) Chromosoma, 109 (6), pp. 365-371
  • Schweigmann, N., Vallvé, S., Muscio, O., Guillini, M., Alberti, A., Wisnivesky-Colli, C., Dispersal flight by Triatoma infestans in an arid area of Argentina (1988) Med. Vet. Entomol., 2 (4), pp. 401-404
  • Tajima, F., Evolutionary relationship of DNA sequences in finite populations (1983) Genetics, 105, pp. 437-460
  • Tajima, F., Statistical method for testing the neutral mutation hypothesis by DNA polymorphism (1989) Genetics, 123 (3), pp. 585-595
  • Templeton, A.R., Crandall, K.A., Sing, C.F., A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping and DNA sequence data. III. Cladogram estimation (1992) Genetics, 132 (2), pp. 619-633
  • Torres-Pérez, F., Acuna-Retamar, M., Cook, J.A., Bacigalupo, A., García, A., Cattan, P.E., Statistical phylogeography of Chagas disease vector Triatoma infestans: testing biogeographic hypotheses of dispersal (2010) Infect. Genet. Evol., 11 (1), pp. 167-174
  • True, J.R., Insect melanism: the molecules matter (2003) Trends Ecol. Evol., 18 (12), pp. 640-647
  • Ward, J.P., Baker, P.S., The tethered flight performance of a laboratory population of Triatoma infestans Klug (Hemiptera: Reduviidae) (1982) Bull. Entomol. Res., 72 (1), pp. 17-28
  • Watterson, G.A., On the number of segregating sites in genetical models without recombination (1975) Theor. Popul. Biol., 7, pp. 256-276
  • Weir, B.S., Cockerham, C.C., Estimating F-statistics for the analysis of population structure (1984) Evolution, 38 (6), pp. 1358-1370
  • World Health Organization, Control of Chagas Disease, Second Report of the WHO Expert Committee (2002) WHO Technical Report Series 905, p. 120. , Geneva, Switzerland
  • Van Oosterhout, C., Hutchinson, W.F., Wills, D.P.M., Shipley, P., MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data (2004) Mol. Ecol. Notes, 4 (3), pp. 535-538
  • Yeo, M., Acosta, N., Llewellyn, M., Sánchez, H., Adamson, S., Miles, G., López, E., Miles, M., Origins of Chagas disease: Didelphis species are natural hosts of Trypanosoma cruzi I and armadillos hosts of Trypanosoma cruzi II, including hybrids (2005) Int. J. Parasitol., 35 (2), pp. 225-233
  • Zuccon, A., Zuccon, D., (2010) MrEnt v.2.2. Program Distributed by the Authors, , http://www.mrent.org, (last accessed 31.08.10)

Citas:

---------- APA ----------
Piccinali, R.V., Marcet, P.L., Ceballos, L.A., Kitron, U., Gürtler, R.E. & Dotson, E.M. (2011) . Genetic variability, phylogenetic relationships and gene flow in Triatoma infestans dark morphs from the Argentinean Chaco. Infection, Genetics and Evolution, 11(5), 895-903.
http://dx.doi.org/10.1016/j.meegid.2011.02.013
---------- CHICAGO ----------
Piccinali, R.V., Marcet, P.L., Ceballos, L.A., Kitron, U., Gürtler, R.E., Dotson, E.M. "Genetic variability, phylogenetic relationships and gene flow in Triatoma infestans dark morphs from the Argentinean Chaco" . Infection, Genetics and Evolution 11, no. 5 (2011) : 895-903.
http://dx.doi.org/10.1016/j.meegid.2011.02.013
---------- MLA ----------
Piccinali, R.V., Marcet, P.L., Ceballos, L.A., Kitron, U., Gürtler, R.E., Dotson, E.M. "Genetic variability, phylogenetic relationships and gene flow in Triatoma infestans dark morphs from the Argentinean Chaco" . Infection, Genetics and Evolution, vol. 11, no. 5, 2011, pp. 895-903.
http://dx.doi.org/10.1016/j.meegid.2011.02.013
---------- VANCOUVER ----------
Piccinali, R.V., Marcet, P.L., Ceballos, L.A., Kitron, U., Gürtler, R.E., Dotson, E.M. Genetic variability, phylogenetic relationships and gene flow in Triatoma infestans dark morphs from the Argentinean Chaco. Infec. Genet. Evol. 2011;11(5):895-903.
http://dx.doi.org/10.1016/j.meegid.2011.02.013