Artículo
Abstract:
In order to provide more insight into the function of aquaporins during pollination, we characterized NIP4;1 and NIP4;2, 2 pollen-specific aquaporins of Arabidopsis thaliana. NIP4;1 and NIP4;2 displayed high amino acid identity. RT-PCR and GUS promoter analysis showed that they have different expression patterns. NIP4;1 is expressed at low levels in mature pollen, while NIP4;2 is highly expressed only during pollen tube growth. Single T-DNA nip4;1 and nip4;2 mutants and double amiRNA nip4;1 nip4;2 knockdowns showed reduced male fertility due to deficient pollen germination and pollen tube length. Functional assays in oocytes showed that NIP4;1 and NIP4;2 transport water and nonionic solutes. Here, the participation of the different pollen aquaporins in pollen hydration and pollen tube growth is discussed.
Registro:
| Documento: | Artículo |
| Título: | Pollen aquaporins: What are they there for? |
| Autor: | Pérez Di Giorgio, J.A.; Barberini, M.L.; Amodeo, G.; Muschietti, J.P. |
| Filiación: | c Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes, Pabellón II, Ciudad Universitaria, Buenos Aires, Argentina c Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes, Pabellón II, Ciudad Universitaria, Buenos Aires, Argentina c Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes, Pabellón II, Ciudad Universitaria, Buenos Aires, Argentina |
| Palabras clave: | Aquaporin; pollen; pollen hydration; pollination; aquaporin; Arabidopsis protein; Arabidopsis; genetics; metabolism; physiology; pollen tube; pollination; promoter region; Aquaporins; Arabidopsis; Arabidopsis Proteins; Pollen Tube; Pollination; Promoter Regions, Genetic |
| Año: | 2016 |
| Volumen: | 11 |
| Número: | 9 |
| Página de inicio: | e1217375 |
| DOI: | http://dx.doi.org/10.1080/15592324.2016.1217375 |
| Título revista: | Plant signaling & behavior |
| Título revista abreviado: | Plant Signal Behav |
| ISSN: | 15592324 |
| CAS: | aquaporin, 215587-75-0; Aquaporins; Arabidopsis Proteins |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15592324_v11_n9_pe1217375_PerezDiGiorgio |
Citas:
---------- APA ----------
Pérez Di Giorgio, J.A., Barberini, M.L., Amodeo, G. & Muschietti, J.P. (2016) . Pollen aquaporins: What are they there for?. Plant signaling & behavior, 11(9), e1217375.http://dx.doi.org/10.1080/15592324.2016.1217375
---------- CHICAGO ----------
Pérez Di Giorgio, J.A., Barberini, M.L., Amodeo, G., Muschietti, J.P. "Pollen aquaporins: What are they there for?" . Plant signaling & behavior 11, no. 9 (2016) : e1217375.http://dx.doi.org/10.1080/15592324.2016.1217375
---------- MLA ----------
Pérez Di Giorgio, J.A., Barberini, M.L., Amodeo, G., Muschietti, J.P. "Pollen aquaporins: What are they there for?" . Plant signaling & behavior, vol. 11, no. 9, 2016, pp. e1217375.http://dx.doi.org/10.1080/15592324.2016.1217375
---------- VANCOUVER ----------
Pérez Di Giorgio, J.A., Barberini, M.L., Amodeo, G., Muschietti, J.P. Pollen aquaporins: What are they there for?. Plant Signal Behav. 2016;11(9):e1217375.http://dx.doi.org/10.1080/15592324.2016.1217375
