In order to provide more insight into the function of aquaporins during pollination, we characterized NIP4;1 and NIP4;2, 2 pollen-specific aquaporins of Arabidopsis thaliana. NIP4;1 and NIP4;2 displayed high amino acid identity. RT-PCR and GUS promoter analysis showed that they have different expression patterns. NIP4;1 is expressed at low levels in mature pollen, while NIP4;2 is highly expressed only during pollen tube growth. Single T-DNA nip4;1 and nip4;2 mutants and double amiRNA nip4;1 nip4;2 knockdowns showed reduced male fertility due to deficient pollen germination and pollen tube length. Functional assays in oocytes showed that NIP4;1 and NIP4;2 transport water and nonionic solutes. Here, the participation of the different pollen aquaporins in pollen hydration and pollen tube growth is discussed.
Documento: | Artículo |
Título: | Pollen aquaporins: What are they there for? |
Autor: | Pérez Di Giorgio, J.A.; Barberini, M.L.; Amodeo, G.; Muschietti, J.P. |
Filiación: | c Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes, Pabellón II, Ciudad Universitaria, Buenos Aires, Argentina c Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes, Pabellón II, Ciudad Universitaria, Buenos Aires, Argentina c Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes, Pabellón II, Ciudad Universitaria, Buenos Aires, Argentina |
Palabras clave: | Aquaporin; pollen; pollen hydration; pollination; aquaporin; Arabidopsis protein; Arabidopsis; genetics; metabolism; physiology; pollen tube; pollination; promoter region; Aquaporins; Arabidopsis; Arabidopsis Proteins; Pollen Tube; Pollination; Promoter Regions, Genetic |
Año: | 2016 |
Volumen: | 11 |
Número: | 9 |
Página de inicio: | e1217375 |
DOI: | http://dx.doi.org/10.1080/15592324.2016.1217375 |
Título revista: | Plant signaling & behavior |
Título revista abreviado: | Plant Signal Behav |
ISSN: | 15592324 |
CAS: | aquaporin, 215587-75-0; Aquaporins; Arabidopsis Proteins |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15592324_v11_n9_pe1217375_PerezDiGiorgio |