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Rovetta, A.I.; Peña, D.; Hernández Del Pino, R.E.; Recalde, G.M.; Pellegrini, J.; Bigi, F.; Musella, R.M.; Palmero, D.J.; Gutierrez, M.; Colombo, M.I.; García, V.E. "IFNG-mediated immune responses enhance autophagy against Mycobacterium tuberculosis antigens in patients with active tuberculosis" (2014) Autophagy. 10(12):2109-2121
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Abstract:

Protective immunity against Mycobacterium tuberculosis (Mtb) requires IFNG. Besides, IFNG-mediated induction of autophagy suppresses survival of virulent Mtb in macrophage cell lines. We investigated the contribution of autophagy to the defense against Mtb antigen (Mtb-Ag) in cells from tuberculosis patients and healthy donors (HD). Patients were classified as high responders (HR) if their T cells produced significant IFNG against Mtb-Ag; and low responders (LR) when patients showed weak or no T cell responses to Mtb-Ag. The highest autophagy levels were detected in HD cells whereas the lowest quantities were observed in LR patients. Interestingly, upon Mtb-Ag stimulation, we detected a positive correlation between IFNG and MAP1LC3B-II/LC3-II levels. Actually, blockage of Mtb-Ag-induced IFNG markedly reduced autophagy in HR patients whereas addition of limited amounts of IFNG significantly increased autophagy in LR patients. Therefore, autophagy collaborates with human immune responses against Mtb in close association with specific IFNG secreted against the pathogen. © 2014 Taylor & Francis Group, LLC.

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Documento: Artículo
Título:IFNG-mediated immune responses enhance autophagy against Mycobacterium tuberculosis antigens in patients with active tuberculosis
Autor:Rovetta, A.I.; Peña, D.; Hernández Del Pino, R.E.; Recalde, G.M.; Pellegrini, J.; Bigi, F.; Musella, R.M.; Palmero, D.J.; Gutierrez, M.; Colombo, M.I.; García, V.E.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (UBA), Buenos Aires, Argentina
Inst. de Quimica Biologica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN) UBA-CONICET, Buenos Aires, Argentina
Instituto de Histología y Embriología de Mendoza, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo-CONICET, Mendoza, Argentina
Instituto de Biotecnología, CICVyA - INTA, N. Repetto and De los Reseros, Argentina
División Tisioneumonología, Hospital F.J. Muñiz, Buenos Aires, Argentina
Sección Bacteriología de la Tuberculosis, Hospital General De Agudos. E. Tornú, Buenos Aires, Argentina
Palabras clave:Autophagy; Cytokines; Defense; IFNG; Immune response; Patients; Tuberculosis; bacterial antigen; bafilomycin A1; CD14 antigen; gamma interferon receptor; bacterial antigen; gamma interferon; Mycobacterium tuberculosis antigens; adaptive immunity; adherent cell; Article; autophagosome; autophagy; bacterial strain; CD3+ T lymphocyte; cellular immunity; confocal microscopy; controlled study; cytokine production; cytokine release; dendritic cell; densitometry; enzyme linked immunosorbent assay; flow cytometry; fluorescence activated cell sorting; human; human cell; immunofluorescence; innate immunity; macrophage; monocyte; Mycobacterium tuberculosis; peripheral blood mononuclear cell; treatment response; tuberculosis; Western blotting; autophagy; drug effects; female; immunology; male; metabolism; microbiology; Mycobacterium tuberculosis; Th1 cell; tuberculosis; Mycobacterium tuberculosis; Antigens, Bacterial; Antigens, Bacterial; Autophagy; Autophagy; Female; Female; Humans; Humans; Interferon-gamma; Interferon-gamma; Macrophages; Macrophages; Male; Male; Mycobacterium tuberculosis; Mycobacterium tuberculosis; Th1 Cells; Th1 Cells; Tuberculosis; Tuberculosis
Año:2014
Volumen:10
Número:12
Página de inicio:2109
Página de fin:2121
DOI: http://dx.doi.org/10.4161/15548627.2014.981791
Título revista:Autophagy
Título revista abreviado:Autophagy
ISSN:15548627
CAS:bafilomycin A1, 88899-55-2; gamma interferon, 82115-62-6; Antigens, Bacterial; Interferon-gamma; Mycobacterium tuberculosis antigens
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15548627_v10_n12_p2109_Rovetta

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Citas:

---------- APA ----------
Rovetta, A.I., Peña, D., Hernández Del Pino, R.E., Recalde, G.M., Pellegrini, J., Bigi, F., Musella, R.M.,..., García, V.E. (2014) . IFNG-mediated immune responses enhance autophagy against Mycobacterium tuberculosis antigens in patients with active tuberculosis. Autophagy, 10(12), 2109-2121.
http://dx.doi.org/10.4161/15548627.2014.981791
---------- CHICAGO ----------
Rovetta, A.I., Peña, D., Hernández Del Pino, R.E., Recalde, G.M., Pellegrini, J., Bigi, F., et al. "IFNG-mediated immune responses enhance autophagy against Mycobacterium tuberculosis antigens in patients with active tuberculosis" . Autophagy 10, no. 12 (2014) : 2109-2121.
http://dx.doi.org/10.4161/15548627.2014.981791
---------- MLA ----------
Rovetta, A.I., Peña, D., Hernández Del Pino, R.E., Recalde, G.M., Pellegrini, J., Bigi, F., et al. "IFNG-mediated immune responses enhance autophagy against Mycobacterium tuberculosis antigens in patients with active tuberculosis" . Autophagy, vol. 10, no. 12, 2014, pp. 2109-2121.
http://dx.doi.org/10.4161/15548627.2014.981791
---------- VANCOUVER ----------
Rovetta, A.I., Peña, D., Hernández Del Pino, R.E., Recalde, G.M., Pellegrini, J., Bigi, F., et al. IFNG-mediated immune responses enhance autophagy against Mycobacterium tuberculosis antigens in patients with active tuberculosis. Autophagy. 2014;10(12):2109-2121.
http://dx.doi.org/10.4161/15548627.2014.981791