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Abstract:

The gradient of Bicoid (Bcd) is key for the establishment of the anterior-posterior axis in Drosophila embryos. The gradient properties are compatible with the SDD model in which Bcd is synthesized at the anterior pole and then diffuses into the embryo and is degraded with a characteristic time. Within this model, the Bcd diffusion coefficient is critical to set the timescale of gradient formation. This coefficient has been measured using two optical techniques, Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS), obtaining estimates in which the FCS value is an order of magnitude larger than the FRAP one. This discrepancy raises the following questions: which estimate is "correct" what is the reason for the disparity; and can the SDD model explain Bcd gradient formation within the experimentally observed times? In this paper, we use a simple biophysical model in which Bcd diffuses and interacts with binding sites to show that both the FRAP and the FCS estimates may be correct and compatible with the observed timescale of gradient formation. The discrepancy arises from the fact that FCS and FRAP report on different effective (concentration dependent) diffusion coefficients, one of which describes the spreading rate of the individual Bcd molecules (the messengers) and the other one that of their concentration (the message). The latter is the one that is more relevant for the gradient establishment and is compatible with its formation within the experimentally observed times. © 2014 Sigaut et al.

Registro:

Documento: Artículo
Título:Messages Do Diffuse Faster than Messengers: Reconciling Disparate Estimates of the Morphogen Bicoid Diffusion Coefficient
Autor:Sigaut, L.; Pearson, J.E.; Colman-Lerner, A.; Ponce Dawson, S.
Filiación:Departamento de Física and IFIBA, FCEN-UBA - CONICET, Buenos Aires, Argentina
Theoretical Biology and Biophysics, Los Alamos National Laboratory, Los Alamos, NM, United States
Departamento de Fisiología, Biología Molecular y Celular and IFIBYNE, CONICET, FCEN-UBA, Buenos Aires, Argentina
Palabras clave:bicoid; enhanced green fluorescent protein; morphogen; unclassified drug; bicoid protein, Drosophila; Drosophila protein; homeodomain protein; hybrid protein; transactivator protein; article; binding site; concentration (parameters); diffusion coefficient; Drosophila; embryo; fluorescence correlation spectroscopy; fluorescence recovery after photobleaching; mathematical model; molecular dynamics; nonhuman; validation process; active transport; animal; biological model; biology; biophysics; computer simulation; diffusion; Drosophila melanogaster; embryology; genetics; metabolism; morphogenesis; spectrofluorometry; transgenic animal; Active Transport, Cell Nucleus; Animals; Animals, Genetically Modified; Biophysical Phenomena; Body Patterning; Computational Biology; Computer Simulation; Diffusion; Drosophila melanogaster; Drosophila Proteins; Fluorescence Recovery After Photobleaching; Homeodomain Proteins; Models, Biological; Recombinant Fusion Proteins; Spectrometry, Fluorescence; Trans-Activators
Año:2014
Volumen:10
Número:6
DOI: http://dx.doi.org/10.1371/journal.pcbi.1003629
Título revista:PLoS Computational Biology
Título revista abreviado:PLoS Comput. Biol.
ISSN:1553734X
CAS:bicoid protein, Drosophila; Drosophila Proteins; Homeodomain Proteins; Recombinant Fusion Proteins; Trans-Activators
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1553734X_v10_n6_p_Sigaut

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Citas:

---------- APA ----------
Sigaut, L., Pearson, J.E., Colman-Lerner, A. & Ponce Dawson, S. (2014) . Messages Do Diffuse Faster than Messengers: Reconciling Disparate Estimates of the Morphogen Bicoid Diffusion Coefficient. PLoS Computational Biology, 10(6).
http://dx.doi.org/10.1371/journal.pcbi.1003629
---------- CHICAGO ----------
Sigaut, L., Pearson, J.E., Colman-Lerner, A., Ponce Dawson, S. "Messages Do Diffuse Faster than Messengers: Reconciling Disparate Estimates of the Morphogen Bicoid Diffusion Coefficient" . PLoS Computational Biology 10, no. 6 (2014).
http://dx.doi.org/10.1371/journal.pcbi.1003629
---------- MLA ----------
Sigaut, L., Pearson, J.E., Colman-Lerner, A., Ponce Dawson, S. "Messages Do Diffuse Faster than Messengers: Reconciling Disparate Estimates of the Morphogen Bicoid Diffusion Coefficient" . PLoS Computational Biology, vol. 10, no. 6, 2014.
http://dx.doi.org/10.1371/journal.pcbi.1003629
---------- VANCOUVER ----------
Sigaut, L., Pearson, J.E., Colman-Lerner, A., Ponce Dawson, S. Messages Do Diffuse Faster than Messengers: Reconciling Disparate Estimates of the Morphogen Bicoid Diffusion Coefficient. PLoS Comput. Biol. 2014;10(6).
http://dx.doi.org/10.1371/journal.pcbi.1003629