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Abstract:

Background: Innovative thinking and experimentation were the hallmarks of Mack Fulwyler's approach to research. This report summarizes some of the ideas and their early realizations that he pursued in the field of imaging cytometry, work that was not published before his untimely death, although he composed the initial draft of this report. Methods: Included are related experiments implemented in the programmable array microscope (PAM) devised for patterned illumination and detection, the instrument that Mack Fulwyler employed during a sabbatical leave in Göttingen in 1998. Despite being the originator of instrumentation for flow cytometry and sorting, Mack Fulwyler was intensely interested in imaging systems, recognizing their ability to resolve cellular details obscured by the whole cell signals generally acquired in flow. At one point, these interests merged with those of two other authors (I.T.Y. and T.M.J.), leading to the Image Cytometry and Sorting (ICAS) strategy and project. A major goal was uncomplicated rare cell detection and isolation using a sequential process of cellular labeling via suitable probes, whole field imaging, and selective area-restricted photoinduced reactions designed to encapsulate and/or chemically or physically tag cells in a manner permitting subsequent fractionation by bulk techniques. Results and Conclusion: This publication features photo-induced polymerization, photodecaging, photoactivation, and photochromic conversion reactions carried out by Fulwyler and/or the other authors with the PAM, employing operator designated patterns and locations in various samples. Photopolymerization of polyethylene glycol-diacrylate to a gel-like structure allowing the specific selection of objects (cells) for further analysis and processing techniques was the approach explored personally by Mack Fulwyler in relation to the ICAS concept. © 2005 International Society for Analytical Cytology.

Registro:

Documento: Artículo
Título:Selective photoreactions in a programmable array microscope (PAM): Photoinitiated polymerization, photodecaging, and photochromic conversion
Autor:Fulwyler, M.; Hanley, Q.S.; Schnetter, C.; Young, I.T.; Jares-Erijman, E.A.; Arndt-Jovin, D.J.; Jovin, T.M.
Filiación:Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany
School of Biomedical and Natural Sciences, Nottingham Trent University, Nottingham, United Kingdom
Department of Imaging Science and Technology, Faculty of Applied Sciences, Delft University of Technology, Delft, Netherlands
Departamento de Química Orgánica, Ciudad Universitaria, Universidad de Buenos Aires, Buenos Aires, Argentina
Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D-37077 Göttingen, Germany
Palabras clave:Confocal microscopy; Image Cytometry and Sorting; Optical sectioning; Photochromic fluorescence resonance energy transfer; Photogelation; acrylic acid derivative; macrogol; article; biotransformation; cell encapsulation; cell labeling; gel; illumination; image cytometry; instrumentation; medical research; microscope; photoreactivity; polymerization; priority journal; programmable array microscope; technique; 3T3 Cells; Animals; Biological Transport; Dextrans; Fibroblasts; Gels; Imaging, Three-Dimensional; Mice; Microscopy; Microspheres; Photochemistry; Polymers
Año:2005
Volumen:67
Número:2
Página de inicio:68
Página de fin:75
DOI: http://dx.doi.org/10.1002/cyto.a.20174
Título revista:Cytometry Part A
Título revista abreviado:Cytometry Part A
ISSN:15524922
CODEN:CPAYA
CAS:macrogol, 25322-68-3; Dextrans, 9004-54-0; Gels; Polymers
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15524922_v67_n2_p68_Fulwyler

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Citas:

---------- APA ----------
Fulwyler, M., Hanley, Q.S., Schnetter, C., Young, I.T., Jares-Erijman, E.A., Arndt-Jovin, D.J. & Jovin, T.M. (2005) . Selective photoreactions in a programmable array microscope (PAM): Photoinitiated polymerization, photodecaging, and photochromic conversion. Cytometry Part A, 67(2), 68-75.
http://dx.doi.org/10.1002/cyto.a.20174
---------- CHICAGO ----------
Fulwyler, M., Hanley, Q.S., Schnetter, C., Young, I.T., Jares-Erijman, E.A., Arndt-Jovin, D.J., et al. "Selective photoreactions in a programmable array microscope (PAM): Photoinitiated polymerization, photodecaging, and photochromic conversion" . Cytometry Part A 67, no. 2 (2005) : 68-75.
http://dx.doi.org/10.1002/cyto.a.20174
---------- MLA ----------
Fulwyler, M., Hanley, Q.S., Schnetter, C., Young, I.T., Jares-Erijman, E.A., Arndt-Jovin, D.J., et al. "Selective photoreactions in a programmable array microscope (PAM): Photoinitiated polymerization, photodecaging, and photochromic conversion" . Cytometry Part A, vol. 67, no. 2, 2005, pp. 68-75.
http://dx.doi.org/10.1002/cyto.a.20174
---------- VANCOUVER ----------
Fulwyler, M., Hanley, Q.S., Schnetter, C., Young, I.T., Jares-Erijman, E.A., Arndt-Jovin, D.J., et al. Selective photoreactions in a programmable array microscope (PAM): Photoinitiated polymerization, photodecaging, and photochromic conversion. Cytometry Part A. 2005;67(2):68-75.
http://dx.doi.org/10.1002/cyto.a.20174