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Abstract:

This paper analyzes, in the toad Bufo arenarum, the effect on spermiation and androgen secretion of two human recombinant gonadotropins, human recombinant LH (hrLH) and human recombinant FSH (hrFSH) as well as the well-known spermiation-inducing hormone, human chorionic gonadotropin (hCG). For this purpose, testes were incubated with different concentrations of hrLH (0.01-2.5 μg/ml) and hrFSH (0.05-5 μg/ml), and results were compared with those obtained with 2.5 μg/ml hCG. Spermiation was most efficiently stimulated by hrFSH, which elicited a higher response than either hrLH or hCG. Both hrFSH and hrLH produced a bell-shaped dose-response curve, with a 50% inhibition on spermiation at a concentration twice higher than that necessary to get the highest response. However, none of the gonadotropins yielded a biphasic response on androgen secretion, hrLH producing the highest response at a concentration that evoked a 70% inhibition in the spermiation test. Regarding steroidogenesis, hrLH and hrFSH were more active than hCG. Taken together, the results described in this paper suggest that, in B. arenarum, spermiation and androgen secretion are mediated by different receptors. After comparing the effects of recombinant hormones, we conclude that hrFSH has a greater effect on spermiation than hCG or hrLH. © 2005 Wiley-Liss, Inc.

Registro:

Documento: Artículo
Título:Effect of human gonadotropins on spermiation and androgen biosynthesis in the testis of the toad Bufo arenarum (Amphibia, Anura)
Autor:Pozzi, A.G.; Rosemblit, C.; Ceballos, N.R.
Filiación:Laboratorio de Endocrinología Comparada and PRHOM-CONICET, Departamento de Biodiversidad Y Biología Experimental, Pabellón II, 1428 Buenos Aires, Argentina
Laboratorio de Endocrinología Comparada, Departamento de Biodiversidad Y Biología Experimental, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
Palabras clave:Amphibia; Anura; Bufo arenarum; chorionic gonadotropin; follitropin; luteinizing hormone; testosterone; animal; article; biosynthesis; comparative study; dose response; drug effect; human; male; physiology; secretion; spermatogenesis; spermatozoon; testis; toad; Animals; Bufo arenarum; Chorionic Gonadotropin; Dose-Response Relationship, Drug; Follicle Stimulating Hormone; Humans; Luteinizing Hormone; Male; Spermatogenesis; Spermatozoa; Testis; Testosterone
Año:2006
Volumen:305 A
Número:1
Página de inicio:96
Página de fin:102
DOI: http://dx.doi.org/10.1002/jez.a.254
Título revista:Journal of Experimental Zoology Part A: Comparative Experimental Biology
Título revista abreviado:J. Exp. Zool. Part A Comp. Exp. Biol.
ISSN:15488969
CAS:chorionic gonadotropin, 9002-61-3; follitropin, 9002-68-0; luteinizing hormone, 39341-83-8, 9002-67-9; testosterone, 58-22-0; Chorionic Gonadotropin; Follicle Stimulating Hormone, 9002-68-0; Luteinizing Hormone, 9002-67-9; Testosterone, 58-22-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15488969_v305A_n1_p96_Pozzi

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Citas:

---------- APA ----------
Pozzi, A.G., Rosemblit, C. & Ceballos, N.R. (2006) . Effect of human gonadotropins on spermiation and androgen biosynthesis in the testis of the toad Bufo arenarum (Amphibia, Anura). Journal of Experimental Zoology Part A: Comparative Experimental Biology, 305 A(1), 96-102.
http://dx.doi.org/10.1002/jez.a.254
---------- CHICAGO ----------
Pozzi, A.G., Rosemblit, C., Ceballos, N.R. "Effect of human gonadotropins on spermiation and androgen biosynthesis in the testis of the toad Bufo arenarum (Amphibia, Anura)" . Journal of Experimental Zoology Part A: Comparative Experimental Biology 305 A, no. 1 (2006) : 96-102.
http://dx.doi.org/10.1002/jez.a.254
---------- MLA ----------
Pozzi, A.G., Rosemblit, C., Ceballos, N.R. "Effect of human gonadotropins on spermiation and androgen biosynthesis in the testis of the toad Bufo arenarum (Amphibia, Anura)" . Journal of Experimental Zoology Part A: Comparative Experimental Biology, vol. 305 A, no. 1, 2006, pp. 96-102.
http://dx.doi.org/10.1002/jez.a.254
---------- VANCOUVER ----------
Pozzi, A.G., Rosemblit, C., Ceballos, N.R. Effect of human gonadotropins on spermiation and androgen biosynthesis in the testis of the toad Bufo arenarum (Amphibia, Anura). J. Exp. Zool. Part A Comp. Exp. Biol. 2006;305 A(1):96-102.
http://dx.doi.org/10.1002/jez.a.254