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Abstract:

Murine embryonic stem cells (mESCs) are pluripotent cells that can be propagated in an undifferentiated state in continuous culture on a feeder layer or without feeders in the presence of leukemia inhibitory factor (LIF). Although there has been a great advance since their establishment, ESC culture is still complex and expensive. Therefore, finding culture conditions that maintain the self-renewal of ESCs, preventing their differentiation and promoting their proliferation, is still an area of great interest. In this work, we studied the effects of the conditioned medium from a bovine granulosa cell line (BGC-CM) on the maintenance of self-renewal and pluripotency of mESCs. We found that this medium is able to maintain mESCs' self-renewal while preserving its critical properties without LIF addition. mESCs cultured in BGC-CM expressed the stem cell markers Oct4, Sox2, Nanog, SSEA-1, Klf4, Rex1, and ECAT1. Moreover, mESCs cultured in BGC-CM gave rise to embryoid bodies and teratomas that differentiated effectively to diverse cell populations from endoderm, mesoderm, and ectoderm. Further, we found that mESCs cultured in BGC-CM have an increased proliferation rate compared with cells grown in the mESC standard culture medium supplemented with LIF. These findings may provide a powerful tool to culture mESCs for long periods of time with high proliferation rate while preserving its basic characteristics, contributing to the application of these cells to assess potential tissue engineering and cellular therapy applications. © Copyright 2011, Mary Ann Liebert, Inc.

Registro:

Documento: Artículo
Título:Maintenance of murine embryonic stem cells' self-renewal and pluripotency with increase in proliferation rate by a bovine granulosa cell line-conditioned medium
Autor:Losino, N.; Luzzani, C.; Solari, C.; Boffi, J.; Tisserand, M.L.; Sevlever, G.; Barañao, L.; Guberman, A.
Filiación:Lab. de Regulacion de la Expresion Genica en el Crecimiento Supervivencia y Diferenciacion Celular, Departamento de Química Biológica, Ciudad Universitaria, Intendente Guiraldes 2160, Pab. 2, 4to piso, QB-71, Buenos Aires C1428EGA, Argentina
Laboratorio de Biología del Desarrollo Celular, Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia (FLENI), Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, CONICET, Buenos Aires, Argentina
Palabras clave:kruppel like factor 4; octamer transcription factor 4; Rex protein; stage specific embryo antigen 1; transcription factor NANOG; transcription factor Sox2; animal cell; article; cell culture; cell differentiation; cell growth; cell population; cell proliferation; cell renewal; cow; culture medium; ectoderm; embryoid body; embryonic stem cell; endoderm; granulosa cell; mesoderm; nonhuman; pluripotent stem cell; priority journal; teratoma; Animals; Biological Markers; Cattle; Cell Culture Techniques; Cell Differentiation; Cell Line; Cell Proliferation; Culture Media, Conditioned; Embryonic Stem Cells; Female; Granulosa Cells; Mice; Pluripotent Stem Cells; Bovinae; Murinae
Año:2011
Volumen:20
Número:8
Página de inicio:1439
Página de fin:1449
DOI: http://dx.doi.org/10.1089/scd.2010.0336
Título revista:Stem Cells and Development
Título revista abreviado:Stem Cells Dev.
ISSN:15473287
CODEN:SCDTA
CAS:Biological Markers; Culture Media, Conditioned
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15473287_v20_n8_p1439_Losino

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Citas:

---------- APA ----------
Losino, N., Luzzani, C., Solari, C., Boffi, J., Tisserand, M.L., Sevlever, G., Barañao, L.,..., Guberman, A. (2011) . Maintenance of murine embryonic stem cells' self-renewal and pluripotency with increase in proliferation rate by a bovine granulosa cell line-conditioned medium. Stem Cells and Development, 20(8), 1439-1449.
http://dx.doi.org/10.1089/scd.2010.0336
---------- CHICAGO ----------
Losino, N., Luzzani, C., Solari, C., Boffi, J., Tisserand, M.L., Sevlever, G., et al. "Maintenance of murine embryonic stem cells' self-renewal and pluripotency with increase in proliferation rate by a bovine granulosa cell line-conditioned medium" . Stem Cells and Development 20, no. 8 (2011) : 1439-1449.
http://dx.doi.org/10.1089/scd.2010.0336
---------- MLA ----------
Losino, N., Luzzani, C., Solari, C., Boffi, J., Tisserand, M.L., Sevlever, G., et al. "Maintenance of murine embryonic stem cells' self-renewal and pluripotency with increase in proliferation rate by a bovine granulosa cell line-conditioned medium" . Stem Cells and Development, vol. 20, no. 8, 2011, pp. 1439-1449.
http://dx.doi.org/10.1089/scd.2010.0336
---------- VANCOUVER ----------
Losino, N., Luzzani, C., Solari, C., Boffi, J., Tisserand, M.L., Sevlever, G., et al. Maintenance of murine embryonic stem cells' self-renewal and pluripotency with increase in proliferation rate by a bovine granulosa cell line-conditioned medium. Stem Cells Dev. 2011;20(8):1439-1449.
http://dx.doi.org/10.1089/scd.2010.0336