Artículo

Hofer, E.L.; Labovsky, V.; La Russa, V.; Vallone, V.F.; Honegger, A.E.; Belloc, C.G.; Wen, H.C.; Bordenave, R.H.; Bullorsky, E.O.; Feldman, L.; Chasseing, N.A. "Mesenchymal stromal cells, colony-forming unit fibroblasts, from bone marrow of untreated advanced breast and lung cancer patients suppress fibroblast colony formation from healthy marrow" (2010) Stem Cells and Development. 19(3):359-369
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Abstract:

We have shown that bone marrow (BM) from untreated advanced lung and breast cancer patients (LCP and BCP) have a reduced number of colony-forming unit fibroblasts (CFU-Fs) or mesenchymal stem cells (MSCs). Factors that regulate the proliferation and differentiation of CFU-F are produced by the patients' BM microenvironment. We have now examined whether conditioned media (CM) from patients' CFU-F-derived stromal cells also inhibits the colony-forming efficiency (CFE) of CFU-F in primary cultures from healthy volunteers (HV)-BM. Thus the number and proliferation potential of HV-CFU-F were also found to be decreased and similar to colony numbers and colony size of patients' CFU-F. Stromal cells from both of these types of colonies appeared relatively larger and lacked the characteristic spindle morphology typically seen in healthy stromal cells. We developed an arbitrary mesenchymal stromal cell maturational index by taking three measures consisting of stromal cell surface area, longitudinal and horizontal axis. All stromal indices derived from HV-CFU-F grown in patients' CM were similar to those from stromal elements derived from patients' CFU-F. These indices were markedly higher than stromal indices typical of HV-CFU-F cultured in healthy CM or standard medium [-medium plus 20% heat-inactivated fetal bovine serum (FBS)]. Patients' CM had increased concentrations of the CFU-F inhibitor, GM-CSF, and low levels of bFGF and Dkk-1, strong promoters of self-renewal of MSCs, compared to the levels quantified in CM from HV-CFU-F. Moreover, the majority of patients' MSCs were unresponsive in standard medium and healthy CM to give CFU-F, indicating that the majority of mesenchymal stromal cells from patients' CFU-F are locked in maturational arrest. These results show that alterations of GM-CSF, bFGF, and Dkk-1 are associated with deficient cloning and maturation arrest of CFU-F. Defective autocrine and paracrine mechanisms may be involved in the BM microenvironments of LCP and BCP. © Mary Ann Liebert, Inc. 2010.

Registro:

Documento: Artículo
Título:Mesenchymal stromal cells, colony-forming unit fibroblasts, from bone marrow of untreated advanced breast and lung cancer patients suppress fibroblast colony formation from healthy marrow
Autor:Hofer, E.L.; Labovsky, V.; La Russa, V.; Vallone, V.F.; Honegger, A.E.; Belloc, C.G.; Wen, H.C.; Bordenave, R.H.; Bullorsky, E.O.; Feldman, L.; Chasseing, N.A.
Filiación:Fellow of the Agencia Nacional de Promociòn Cientìfica, Tecnològica y de Innovaciòn Productiva, Buenos Aires, Argentina
Instituto de Biologìa y Medicina Experimental, Vuelta de Obligado 2490, 1428, Buenos Aires, Argentina
Consejo Nacional de Investigaciones Cientìficas, Técnicas de la Repüblica Argentina (CONICET), New York, United States
Cytotherapy Laboratory, Memorial Sloan-Kettering Cancer Center, New York, United States
Department of Oncology, I.Iriarte Hospital, Buenos Aires, Argentina
Department of Hematology and Bone Marrow Transplantation, Britànico Hospital, Buenos Aires, Argentina
Department of Bone Marrow Transplantation, Fundaciòn Favaloro, Buenos Aires, Argentina
CONICET, Argentina
Palabras clave:dickkopf 1 protein; fibroblast growth factor; granulocyte macrophage colony stimulating factor; article; breast cancer; cancer patient; cell maturation; cell proliferation; cell renewal; cell structure; clinical article; colony formation; controlled study; fibroblast; hematopoietic stem cell; human; human cell; in vitro study; lung cancer; mesenchymal stem cell; microenvironment; micrometastasis; priority journal; Bone Marrow Cells; Breast Neoplasms; Cell Proliferation; Cells, Cultured; Colony-Forming Units Assay; Culture Media, Conditioned; Fibroblast Growth Factor 2; Fibroblasts; Granulocyte-Macrophage Colony-Stimulating Factor; Humans; Intercellular Signaling Peptides and Proteins; Lung Neoplasms; Mesenchymal Stem Cells; Stem Cells; Stromal Cells; Time Factors; Bovinae
Año:2010
Volumen:19
Número:3
Página de inicio:359
Página de fin:369
DOI: http://dx.doi.org/10.1089/scd.2008.0375
Título revista:Stem Cells and Development
Título revista abreviado:Stem Cells Dev.
ISSN:15473287
CODEN:SCDTA
CAS:fibroblast growth factor, 62031-54-3; Culture Media, Conditioned; DKK1 protein, human; Fibroblast Growth Factor 2, 103107-01-3; Granulocyte-Macrophage Colony-Stimulating Factor, 83869-56-1; Intercellular Signaling Peptides and Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15473287_v19_n3_p359_Hofer

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Citas:

---------- APA ----------
Hofer, E.L., Labovsky, V., La Russa, V., Vallone, V.F., Honegger, A.E., Belloc, C.G., Wen, H.C.,..., Chasseing, N.A. (2010) . Mesenchymal stromal cells, colony-forming unit fibroblasts, from bone marrow of untreated advanced breast and lung cancer patients suppress fibroblast colony formation from healthy marrow. Stem Cells and Development, 19(3), 359-369.
http://dx.doi.org/10.1089/scd.2008.0375
---------- CHICAGO ----------
Hofer, E.L., Labovsky, V., La Russa, V., Vallone, V.F., Honegger, A.E., Belloc, C.G., et al. "Mesenchymal stromal cells, colony-forming unit fibroblasts, from bone marrow of untreated advanced breast and lung cancer patients suppress fibroblast colony formation from healthy marrow" . Stem Cells and Development 19, no. 3 (2010) : 359-369.
http://dx.doi.org/10.1089/scd.2008.0375
---------- MLA ----------
Hofer, E.L., Labovsky, V., La Russa, V., Vallone, V.F., Honegger, A.E., Belloc, C.G., et al. "Mesenchymal stromal cells, colony-forming unit fibroblasts, from bone marrow of untreated advanced breast and lung cancer patients suppress fibroblast colony formation from healthy marrow" . Stem Cells and Development, vol. 19, no. 3, 2010, pp. 359-369.
http://dx.doi.org/10.1089/scd.2008.0375
---------- VANCOUVER ----------
Hofer, E.L., Labovsky, V., La Russa, V., Vallone, V.F., Honegger, A.E., Belloc, C.G., et al. Mesenchymal stromal cells, colony-forming unit fibroblasts, from bone marrow of untreated advanced breast and lung cancer patients suppress fibroblast colony formation from healthy marrow. Stem Cells Dev. 2010;19(3):359-369.
http://dx.doi.org/10.1089/scd.2008.0375