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Abstract:

Previously, we reported a deficient cloning capacity of the bone marrow (BM) mesenchymal stem cells to give colony-forming unit fibroblast (CFU-F) and an inefficient confluence capacity of BM stromal cells in advanced untreated lung cancer patients (LCP) and breast cancer patients (BCP). Moreover, a decreased level of bFGF at day 7 in the conditioned media from BM CFU-F cultures was found in both cancer groups when compared to the normal range. The current study was specially undertaken, to evaluate the percentage of subconfluent fibroblasts expressing receptors (R) of interleukin-1 (IL-1), platelet-derived growth factor (PDGF), fibroblast growth factor (FGF), transforming growth factor (TGF-β), epidermal growth factor (EGF), and the proteins c-Fos and c-Myc in BM primary cultures from untreated LCP and BCP. An immunocytochemical study on subconfluent BM fibroblast cultures from 13 healthy patients, 16 LCP, and 8 BCP was performed, using as primary antibodies, anti-type I of IL-1 R (IL-1R-1), anti-α, β chains of PDGF R (PDGFR-α, PDGFR-β), anti-type I of FGF R (FGFR-I), anti-type I, II, and III of TGF-β R (TGF-βR-I, TGF-βR-II, and TGF-βR-III), anti-EGF R, anti-c-Fos, and anti-c-Myc. A diminished percentage of subconfluent fibroblasts expressing PDGFR-α, TGFβR-I, II, III, EGFR, and FGFR-I was found in LCP and BCP compared to healthy patients. A diminished percentage of subconfluent fibroblasts expressing c-Fos and c-Myc was found in patients when compared to healthy patients. The alterations we describe could help to explain the deficiency regarding the proliferative and confluence capacity of BM stroma cells in cancer patients. © Mary Ann Liebert. Inc.

Registro:

Documento: Artículo
Título:Alteration on the expression of IL-1, PDGF, TGF-β, EGF, and FGF receptors and c-Fos and c-Myc proteins in bone marrow mesenchymal stroma cells from advanced untreated lung and breast cancer patients
Autor:Hofer, E.L.; La Russa, V.; Honegger, A.E.; Bullorsky, E.O.; Bordenave, R.H.; Chasseing, N.A.
Filiación:Instituto de Biología y Medicina Experimental, 1428, Buenos Aires, Argentina
Bone Marrow Transplant Laboratory, Tulane Cancer Center, New Orleans, LA 70112, United States
Department of Hematology and Bone Marrow Transplantation, British Hospital, 1280, Capital Federal, Argentina
Department of Oncology, Hospital General de Agudos Dr. Iriarte, Quilmes, Provincia de Buenos Aires, Argentina
Instituto de Biología y Medicina Experimental, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:cytokeratin 20; cytokeratin 7; epidermal growth factor receptor; epithelial membrane antigen; fibroblast growth factor receptor; interleukin 1 receptor; monoclonal antibody; Myc protein; oncoprotein; platelet derived growth factor receptor; polyclonal antibody; protein c fos; transforming growth factor beta receptor; article; bone marrow cell; bone marrow metastasis; breast carcinoma; cancer infiltration; cell proliferation; colony forming unit F; controlled study; fibroblast; human; human cell; human tissue; immunocytochemistry; lung non small cell cancer; mesenchymal stem cell; priority journal; prognosis; protein expression; stroma cell; Animals; Bone Marrow Cells; Breast Neoplasms; Cells, Cultured; Culture Media, Conditioned; Female; Fibroblasts; Humans; Lung Neoplasms; Proto-Oncogene Proteins c-fos; Proto-Oncogene Proteins c-myc; Receptor, Epidermal Growth Factor; Receptors, Fibroblast Growth Factor; Receptors, Interleukin-1; Receptors, Platelet-Derived Growth Factor; Receptors, Transforming Growth Factor beta; Stromal Cells; Tumor Stem Cell Assay
Año:2005
Volumen:14
Número:5
Página de inicio:587
Página de fin:594
DOI: http://dx.doi.org/10.1089/scd.2005.14.587
Título revista:Stem Cells and Development
Título revista abreviado:Stem Cells Dev.
ISSN:15473287
CODEN:SCDTA
CAS:fibroblast growth factor receptor, 153424-51-2; Culture Media, Conditioned; Proto-Oncogene Proteins c-fos; Proto-Oncogene Proteins c-myc; Receptor, Epidermal Growth Factor, EC 2.7.1.112; Receptors, Fibroblast Growth Factor; Receptors, Interleukin-1; Receptors, Platelet-Derived Growth Factor, EC 2.7.1.112; Receptors, Transforming Growth Factor beta
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15473287_v14_n5_p587_Hofer

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Citas:

---------- APA ----------
Hofer, E.L., La Russa, V., Honegger, A.E., Bullorsky, E.O., Bordenave, R.H. & Chasseing, N.A. (2005) . Alteration on the expression of IL-1, PDGF, TGF-β, EGF, and FGF receptors and c-Fos and c-Myc proteins in bone marrow mesenchymal stroma cells from advanced untreated lung and breast cancer patients. Stem Cells and Development, 14(5), 587-594.
http://dx.doi.org/10.1089/scd.2005.14.587
---------- CHICAGO ----------
Hofer, E.L., La Russa, V., Honegger, A.E., Bullorsky, E.O., Bordenave, R.H., Chasseing, N.A. "Alteration on the expression of IL-1, PDGF, TGF-β, EGF, and FGF receptors and c-Fos and c-Myc proteins in bone marrow mesenchymal stroma cells from advanced untreated lung and breast cancer patients" . Stem Cells and Development 14, no. 5 (2005) : 587-594.
http://dx.doi.org/10.1089/scd.2005.14.587
---------- MLA ----------
Hofer, E.L., La Russa, V., Honegger, A.E., Bullorsky, E.O., Bordenave, R.H., Chasseing, N.A. "Alteration on the expression of IL-1, PDGF, TGF-β, EGF, and FGF receptors and c-Fos and c-Myc proteins in bone marrow mesenchymal stroma cells from advanced untreated lung and breast cancer patients" . Stem Cells and Development, vol. 14, no. 5, 2005, pp. 587-594.
http://dx.doi.org/10.1089/scd.2005.14.587
---------- VANCOUVER ----------
Hofer, E.L., La Russa, V., Honegger, A.E., Bullorsky, E.O., Bordenave, R.H., Chasseing, N.A. Alteration on the expression of IL-1, PDGF, TGF-β, EGF, and FGF receptors and c-Fos and c-Myc proteins in bone marrow mesenchymal stroma cells from advanced untreated lung and breast cancer patients. Stem Cells Dev. 2005;14(5):587-594.
http://dx.doi.org/10.1089/scd.2005.14.587