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Alló, M.; Buggiano, V.; Fededa, J.P.; Petrillo, E.; Schor, I.; De La Mata, M.; Agirre, E.; Plass, M.; Eyras, E.; Elela, S.A.; Klinck, R.; Chabot, B.; Kornblihtt, A.R. "Control of alternative splicing through siRNA-mediated transcriptional gene silencing" (2009) Nature Structural and Molecular Biology. 16(7):717-724
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Abstract:

When targeting promoter regions, small interfering RNAs (siRNAs) trigger a previously proposed pathway known as transcriptional gene silencing by promoting heterochromatin formation. Here we show that siRNAs targeting intronic or exonic sequences close to an alternative exon regulate the splicing of that exon. The effect occurred in hepatoma and HeLa cells with siRNA antisense strands designed to enter the silencing pathway, suggesting hybridization with nascent pre-mRNA. Unexpectedly, in HeLa cells the sense strands were also effective, suggesting that an endogenous antisense transcript, detectable in HeLa but not in hepatoma cells, acts as a target. The effect depends on Argonaute-1 and is counterbalanced by factors favoring chromatin opening or transcriptional elongation. The increase in heterochromatin marks (dimethylation at Lys9 and trimethylation at Lys27 of histone H3) at the target site, the need for the heterochromatin-associated protein HP1α and the reduction in RNA polymerase II processivity suggest a mechanism involving the kinetic coupling of transcription and alternative splicing. © 2009 Nature America, Inc. All rights reserved.

Registro:

Documento: Artículo
Título:Control of alternative splicing through siRNA-mediated transcriptional gene silencing
Autor:Alló, M.; Buggiano, V.; Fededa, J.P.; Petrillo, E.; Schor, I.; De La Mata, M.; Agirre, E.; Plass, M.; Eyras, E.; Elela, S.A.; Klinck, R.; Chabot, B.; Kornblihtt, A.R.
Filiación:Laboratorio de Fisiología y Biología Molecular, Departamento de Fisiología, Biología Molecular y Celular, Ciudad Universitaria, Buenos Aires, Argentina
ICREA, Universitat Pompeu Fabra, Barcelona, Spain
Laboratoire de Génomique Fonctionnelle, Faculté de Médecine et des Sciences de la Santé, Université de Sherbrooke, QC, Canada
Palabras clave:argonaute 1 protein; heterochromatin protein 1; heterochromatin protein 1alpha; histone H3; lysine; RNA polymerase II; small interfering RNA; unclassified drug; alternative RNA splicing; article; cell specificity; controlled study; exon; gene expression regulation; gene silencing; HeLa cell; heterochromatin; human; human cell; intron; liver cell carcinoma; methylation; priority journal; promoter region; regulatory mechanism; transcription regulation
Año:2009
Volumen:16
Número:7
Página de inicio:717
Página de fin:724
DOI: http://dx.doi.org/10.1038/nsmb.1620
Título revista:Nature Structural and Molecular Biology
Título revista abreviado:Nat. Struct. Mol. Biol.
ISSN:15459993
CODEN:NSMBC
CAS:lysine, 56-87-1, 6899-06-5, 70-54-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15459993_v16_n7_p717_Allo

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Citas:

---------- APA ----------
Alló, M., Buggiano, V., Fededa, J.P., Petrillo, E., Schor, I., De La Mata, M., Agirre, E.,..., Kornblihtt, A.R. (2009) . Control of alternative splicing through siRNA-mediated transcriptional gene silencing. Nature Structural and Molecular Biology, 16(7), 717-724.
http://dx.doi.org/10.1038/nsmb.1620
---------- CHICAGO ----------
Alló, M., Buggiano, V., Fededa, J.P., Petrillo, E., Schor, I., De La Mata, M., et al. "Control of alternative splicing through siRNA-mediated transcriptional gene silencing" . Nature Structural and Molecular Biology 16, no. 7 (2009) : 717-724.
http://dx.doi.org/10.1038/nsmb.1620
---------- MLA ----------
Alló, M., Buggiano, V., Fededa, J.P., Petrillo, E., Schor, I., De La Mata, M., et al. "Control of alternative splicing through siRNA-mediated transcriptional gene silencing" . Nature Structural and Molecular Biology, vol. 16, no. 7, 2009, pp. 717-724.
http://dx.doi.org/10.1038/nsmb.1620
---------- VANCOUVER ----------
Alló, M., Buggiano, V., Fededa, J.P., Petrillo, E., Schor, I., De La Mata, M., et al. Control of alternative splicing through siRNA-mediated transcriptional gene silencing. Nat. Struct. Mol. Biol. 2009;16(7):717-724.
http://dx.doi.org/10.1038/nsmb.1620