Artículo
Crisp, R.L.; Solari, L.; Gammella, D.; Schvartzman, G.A.; Rapetti, M.C.; Donato, H. "Use of capillary blood to diagnose hereditary spherocytosis" (2012) Pediatric Blood and Cancer. 59(7):1299-1301
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Abstract:
We studied 31 children with hemolytic anemia, or with positive family history for hereditary spherocytosis (HS), to assess the reliability of capillary blood samples for the diagnosis. HS was diagnosed in 20 patients. Cryohemolysis (CH) was positive in 94% and eosin-5'-maleimide flow cytometry in 90% of them, whereas flow cytometric osmotic fragility was positive in 94%. Capillary blood sampling showed to be useful for the diagnosis. Simultaneous use of these three tests allows confirming diagnosis in 100% of patients. The use of very small blood volumes (300μl) allows an earlier diagnosis in neonates and small infants. © 2012 Wiley Periodicals, Inc.
Registro:
| Documento: | Artículo |
| Título: | Use of capillary blood to diagnose hereditary spherocytosis |
| Autor: | Crisp, R.L.; Solari, L.; Gammella, D.; Schvartzman, G.A.; Rapetti, M.C.; Donato, H. |
| Filiación: | División Hematología Clínica, Departamento de Medicina, Hospital Nacional Alejandro Posadas, Buenos Aires, Argentina Laboratorio de Análisis Biológicos, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina Consultorios de Hematología Infantil, Buenos Aires, Argentina Laboratorio de Citometría, Departamento de Diagnóstico, Hospital Nacional Alejandro Posadas, Buenos Aires, Argentina Seccion Hematología/Oncología, Hospital Municipal del Niño de San Justo, San Justo, Buenos Aires, Argentina |
| Palabras clave: | Capillary blood; Flow cytometry; Hereditary spherocytosis; Hypertonic cryohemolysis; Neonatal anemia; Osmotic fragility; bilirubin; article; bilirubin blood level; blood group ABO incompatibility; blood sampling; blood smear; blood volume; capillary blood; child; clinical article; cryohemolysis; early diagnosis; erythrocyte disorder; family history; flow cytometry; hemolysis; hemolytic anemia; hereditary spherocytosis; human; infant; newborn hemolytic disease; osmosis; preschool child; priority journal; reticulocyte count; school child; Anemia, Hemolytic; Blood Specimen Collection; Capillaries; Child; Child, Preschool; Composite Resins; Eosine Yellowish-(YS); Freezing; Hematologic Tests; Hemolysis; Humans; Infant; Infant, Newborn; Osmotic Fragility; Sensitivity and Specificity; Spherocytosis, Hereditary |
| Año: | 2012 |
| Volumen: | 59 |
| Número: | 7 |
| Página de inicio: | 1299 |
| Página de fin: | 1301 |
| DOI: | http://dx.doi.org/10.1002/pbc.24157 |
| Título revista: | Pediatric Blood and Cancer |
| Título revista abreviado: | Pediatr. Blood Cancer |
| ISSN: | 15455009 |
| CODEN: | PBCEA |
| CAS: | bilirubin, 18422-02-1, 635-65-4; Composite Resins; Eosine Yellowish-(YS), 17372-87-1; eosin maleimide, 76296-42-9; flowable hybrid composite |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15455009_v59_n7_p1299_Crisp |
Referencias:
- Crisp, R.L., Solari, L., Vota, D., A prospective study to assess the predictive value for hereditary spherocytosis using five laboratory tests (cryohemolysis test, eosin-5'-maleimide flow cytometry, osmotic fragility test, autohemolysis test, and SDS-PAGE) on 50 hereditary spherocytosis families in Argentina (2011) Ann Hematol, 90, pp. 625-634
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- Crisp, R., Solari, L., Gammela, D., Use of capillary blood to perform the eosin-5'-maleimide flow cytometry (EMA-FC) test: Results are comparable to venous blood (2010) Haematologica, 95, p. 700. , abs 1808
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- Crisp, R., Solari, L., Gammela, D., Flow cytometric osmotic fragility (FC-OF) can replace the traditional osmotic fragility (OF) test? Preliminary results (2010) Haematologica, 95, p. 703. , abs. 1816
Citas:
---------- APA ----------
Crisp, R.L., Solari, L., Gammella, D., Schvartzman, G.A., Rapetti, M.C. & Donato, H. (2012) . Use of capillary blood to diagnose hereditary spherocytosis. Pediatric Blood and Cancer, 59(7), 1299-1301.http://dx.doi.org/10.1002/pbc.24157
---------- CHICAGO ----------
Crisp, R.L., Solari, L., Gammella, D., Schvartzman, G.A., Rapetti, M.C., Donato, H. "Use of capillary blood to diagnose hereditary spherocytosis" . Pediatric Blood and Cancer 59, no. 7 (2012) : 1299-1301.http://dx.doi.org/10.1002/pbc.24157
---------- MLA ----------
Crisp, R.L., Solari, L., Gammella, D., Schvartzman, G.A., Rapetti, M.C., Donato, H. "Use of capillary blood to diagnose hereditary spherocytosis" . Pediatric Blood and Cancer, vol. 59, no. 7, 2012, pp. 1299-1301.http://dx.doi.org/10.1002/pbc.24157
---------- VANCOUVER ----------
Crisp, R.L., Solari, L., Gammella, D., Schvartzman, G.A., Rapetti, M.C., Donato, H. Use of capillary blood to diagnose hereditary spherocytosis. Pediatr. Blood Cancer. 2012;59(7):1299-1301.http://dx.doi.org/10.1002/pbc.24157
