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Abstract:

The reversible modulation of the emission of CdSe/ZnS semiconductor nanocrystals (quantum dots) was achieved by binding photochromic diheteroarylethenes and switchable acceptors for fluorescence resonance energy transfer. A biotinylated diheteroarylethene derivative was bound to quantum dots bearing conjugated streptavidin, leading to an intensity decrease as a consequence of energy transfer to the closed form of the acceptor. Interconversion between the open and closed forms by irradiation with 365 and 546 nm light enabled deactivation and activation, respectively, of the FRET process with a corresponding modulation of quantum dot emission, observed both in solution and by sequential wide-field imaging.

Registro:

Documento: Artículo
Título:Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET)
Autor:Jares-Erijman, E.; Giordano, L.; Spagnuolo, C.; Lidke, K.; Jovin, T.M.
Filiación:Departamento de Quńmica Orgánica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria-Pabellón, Buenos Aires, Argentina
Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg, Göttingen, Germany
Departamento de Quńmica Orgánica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria-Pabellón II, 1428 Buenos Aires, Argentina
Palabras clave:Energy transfer; Fluorescence; Imaging techniques; Nanostructured materials; Photochromism; Resonance; Conjugated streptavidin; Diheteroarylethene derivatives; Photochromic fluorescence resonance energy transfer (pcFRET); Semiconductor nanocrystals; Semiconductor quantum dots
Año:2005
Volumen:430
Página de inicio:257
Página de fin:265
DOI: http://dx.doi.org/10.1080/15421400590946479
Título revista:Molecular Crystals and Liquid Crystals
Título revista abreviado:Mol. Cryst. Liq. Cryst.
ISSN:15421406
CODEN:MCLCD
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15421406_v430_n_p257_JaresErijman

Referencias:

  • Lidke, D.S., Arndt-Jovin, D.J., (2004) Physiology, , in press
  • Jares-Erijman, E.A., Song, L., Jovin, T.M., (1997) Molec. Cryst. Liq. Cryst., 298, p. 151
  • Giordano, L., Macareno, J., Song, L., Jovin, T.M., Irie, M., Jares-Erijman, E.A., (2000) Molecules, 5, p. 591
  • Giordano, L., Jovin, T.M., Irie, M., Jares-Erijman, E.A., (2002) J. Amer. Chem. Soc., 124, p. 7481
  • Song, L., Jares-Erijman, E.A., Jovin, T.M., (2002) J. Photochem. Photobiol. A, 150, p. 177
  • Jares-Erijman, E.A., Jovin, T.M., (2003) Nat. Biotechnol., 21, p. 1387
  • Jares-Erijman, E.A., Giordano, L., Spagnuolo, C., Kawior, J., Vermeij, R.J., Jovin, T.M., (2004) SPIE Proc., 5323, p. 13
  • Medintz, I.L., Clapp, A.R., Mattoussi, H., Goldman, E.R., Fisher, B., Mauro, J.M., (2003) Nat. Mater., 2, p. 630
  • Medintz, I.L., Trammell, S.A., Mattoussi, H., Mauro, J.M., (2004) J. Amer. Chem. Soc., 126, p. 30

Citas:

---------- APA ----------
Jares-Erijman, E., Giordano, L., Spagnuolo, C., Lidke, K. & Jovin, T.M. (2005) . Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET). Molecular Crystals and Liquid Crystals, 430, 257-265.
http://dx.doi.org/10.1080/15421400590946479
---------- CHICAGO ----------
Jares-Erijman, E., Giordano, L., Spagnuolo, C., Lidke, K., Jovin, T.M. "Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET)" . Molecular Crystals and Liquid Crystals 430 (2005) : 257-265.
http://dx.doi.org/10.1080/15421400590946479
---------- MLA ----------
Jares-Erijman, E., Giordano, L., Spagnuolo, C., Lidke, K., Jovin, T.M. "Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET)" . Molecular Crystals and Liquid Crystals, vol. 430, 2005, pp. 257-265.
http://dx.doi.org/10.1080/15421400590946479
---------- VANCOUVER ----------
Jares-Erijman, E., Giordano, L., Spagnuolo, C., Lidke, K., Jovin, T.M. Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET). Mol. Cryst. Liq. Cryst. 2005;430:257-265.
http://dx.doi.org/10.1080/15421400590946479