Abstract:
The reversible modulation of the emission of CdSe/ZnS semiconductor nanocrystals (quantum dots) was achieved by binding photochromic diheteroarylethenes and switchable acceptors for fluorescence resonance energy transfer. A biotinylated diheteroarylethene derivative was bound to quantum dots bearing conjugated streptavidin, leading to an intensity decrease as a consequence of energy transfer to the closed form of the acceptor. Interconversion between the open and closed forms by irradiation with 365 and 546 nm light enabled deactivation and activation, respectively, of the FRET process with a corresponding modulation of quantum dot emission, observed both in solution and by sequential wide-field imaging.
Registro:
Documento: |
Artículo
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Título: | Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET) |
Autor: | Jares-Erijman, E.; Giordano, L.; Spagnuolo, C.; Lidke, K.; Jovin, T.M. |
Filiación: | Departamento de Quńmica Orgánica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria-Pabellón, Buenos Aires, Argentina Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg, Göttingen, Germany Departamento de Quńmica Orgánica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria-Pabellón II, 1428 Buenos Aires, Argentina
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Palabras clave: | Energy transfer; Fluorescence; Imaging techniques; Nanostructured materials; Photochromism; Resonance; Conjugated streptavidin; Diheteroarylethene derivatives; Photochromic fluorescence resonance energy transfer (pcFRET); Semiconductor nanocrystals; Semiconductor quantum dots |
Año: | 2005
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Volumen: | 430
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Página de inicio: | 257
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Página de fin: | 265
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DOI: |
http://dx.doi.org/10.1080/15421400590946479 |
Título revista: | Molecular Crystals and Liquid Crystals
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Título revista abreviado: | Mol. Cryst. Liq. Cryst.
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ISSN: | 15421406
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CODEN: | MCLCD
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15421406_v430_n_p257_JaresErijman |
Referencias:
- Lidke, D.S., Arndt-Jovin, D.J., (2004) Physiology, , in press
- Jares-Erijman, E.A., Song, L., Jovin, T.M., (1997) Molec. Cryst. Liq. Cryst., 298, p. 151
- Giordano, L., Macareno, J., Song, L., Jovin, T.M., Irie, M., Jares-Erijman, E.A., (2000) Molecules, 5, p. 591
- Giordano, L., Jovin, T.M., Irie, M., Jares-Erijman, E.A., (2002) J. Amer. Chem. Soc., 124, p. 7481
- Song, L., Jares-Erijman, E.A., Jovin, T.M., (2002) J. Photochem. Photobiol. A, 150, p. 177
- Jares-Erijman, E.A., Jovin, T.M., (2003) Nat. Biotechnol., 21, p. 1387
- Jares-Erijman, E.A., Giordano, L., Spagnuolo, C., Kawior, J., Vermeij, R.J., Jovin, T.M., (2004) SPIE Proc., 5323, p. 13
- Medintz, I.L., Clapp, A.R., Mattoussi, H., Goldman, E.R., Fisher, B., Mauro, J.M., (2003) Nat. Mater., 2, p. 630
- Medintz, I.L., Trammell, S.A., Mattoussi, H., Mauro, J.M., (2004) J. Amer. Chem. Soc., 126, p. 30
Citas:
---------- APA ----------
Jares-Erijman, E., Giordano, L., Spagnuolo, C., Lidke, K. & Jovin, T.M.
(2005)
. Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET). Molecular Crystals and Liquid Crystals, 430, 257-265.
http://dx.doi.org/10.1080/15421400590946479---------- CHICAGO ----------
Jares-Erijman, E., Giordano, L., Spagnuolo, C., Lidke, K., Jovin, T.M.
"Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET)"
. Molecular Crystals and Liquid Crystals 430
(2005) : 257-265.
http://dx.doi.org/10.1080/15421400590946479---------- MLA ----------
Jares-Erijman, E., Giordano, L., Spagnuolo, C., Lidke, K., Jovin, T.M.
"Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET)"
. Molecular Crystals and Liquid Crystals, vol. 430, 2005, pp. 257-265.
http://dx.doi.org/10.1080/15421400590946479---------- VANCOUVER ----------
Jares-Erijman, E., Giordano, L., Spagnuolo, C., Lidke, K., Jovin, T.M. Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET). Mol. Cryst. Liq. Cryst. 2005;430:257-265.
http://dx.doi.org/10.1080/15421400590946479