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Palermo, A.M.; Reynoso, A.S.; López Nigro, M.; Carballo, M.A.; Mudry, M.D. "Teratogenic Evaluation of Metronidazole and Ornidazole Using Drosophila melanogaster as an Experimental Model" (2004) Birth Defects Research Part A - Clinical and Molecular Teratology. 70(4):157-162
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Abstract:

BACKGROUND: Drosophila and vertebrates show similarities that suggest that the mechanisms involved in the induction of developmental defects may be similar in both. Therefore, Drosophila has been proposed as a useful, rapid, and economical model in the preliminary screening for teratology studies. The objective of the present study was to investigate the effect of metronidazole (MTZ) and omidazole (ONZ) on the developmental stages of Drosophila melanogaster. METHODS: Samarkand wild-type females were allowed to lay eggs for 24 hr in media containing MTZ or ONZ at concentrations of 0, 500, 1000, and 2000 μg/ml. When larvae completed their development, the emerging flies were counted and examined for morphological abnormalities. RESULTS: After the analysis of 400-1000 flies for each concentration, ONZ-treated flies did not show an incidence of malformations above control values, although a significant high number of individuals with reduced body size was observed (p < 0.005, χ2 test). On the other hand, the 1000- and 2000-μg/ml MTZ-treated series presented higher frequencies of total abnormalities than did concurrent and historic controls (p < 0.05, χ2 test), indicating an MTZ effect during developmental morphogenesis. CONCLUSIONS: These findings contribute to the characterization of both nitroimidazoles, which are widely used, especially in underdeveloped countries. At the same time, this Drosophila bioassay is sensitive enough to detect differential effects of MTZ and ONZ (abnormalities vs. growth effects), showing specificity and selectivity. © 2004 Wiley-Liss, Inc.

Registro:

Documento: Artículo
Título:Teratogenic Evaluation of Metronidazole and Ornidazole Using Drosophila melanogaster as an Experimental Model
Autor:Palermo, A.M.; Reynoso, A.S.; López Nigro, M.; Carballo, M.A.; Mudry, M.D.
Filiación:CITEFA, Inst. Invest. Cie./Tec. de las FFAA, Buenos Aires, Argentina
Dpto. Ecologia, Genetica y Evolucion, Fac. Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Ctro. de Invest. en Genet. Toxicol., Fac. Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina
CITEFA, DEIMAT-Section ESEM, Juan Bautista de La Salle 4397, B1603ALO, Buenos Aires, Argentina
Palabras clave:metronidazole; nitroimidazole derivative; ornidazole; animal cell; animal experiment; animal model; animal tissue; article; bioassay; concentration response; controlled study; developing country; developmental biology; developmental disorder; Drosophila melanogaster; evaluation; experimental model; female; incidence; larval development; morphological trait; newborn; nonhuman; priority journal; regulatory mechanism; screening test; sensitivity analysis; sensitivity and specificity; teratogenicity; toxicity testing; vertebrate; wild type; Abdomen; Abnormalities, Drug-Induced; Animals; Antiprotozoal Agents; Drosophila melanogaster; Larva; Metronidazole; Ornidazole; Thorax; Drosophila melanogaster; Melanogaster; Vertebrata
Año:2004
Volumen:70
Número:4
Página de inicio:157
Página de fin:162
DOI: http://dx.doi.org/10.1002/bdra.20008
Título revista:Birth Defects Research Part A - Clinical and Molecular Teratology
Título revista abreviado:Birth Defects Res. Part A Clin. Mol. Teratol.
ISSN:15420752
CODEN:BDRPB
CAS:metronidazole, 39322-38-8, 443-48-1; ornidazole, 16773-42-5; Antiprotozoal Agents; Metronidazole, 443-48-1; Ornidazole, 16773-42-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15420752_v70_n4_p157_Palermo

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Citas:

---------- APA ----------
Palermo, A.M., Reynoso, A.S., López Nigro, M., Carballo, M.A. & Mudry, M.D. (2004) . Teratogenic Evaluation of Metronidazole and Ornidazole Using Drosophila melanogaster as an Experimental Model. Birth Defects Research Part A - Clinical and Molecular Teratology, 70(4), 157-162.
http://dx.doi.org/10.1002/bdra.20008
---------- CHICAGO ----------
Palermo, A.M., Reynoso, A.S., López Nigro, M., Carballo, M.A., Mudry, M.D. "Teratogenic Evaluation of Metronidazole and Ornidazole Using Drosophila melanogaster as an Experimental Model" . Birth Defects Research Part A - Clinical and Molecular Teratology 70, no. 4 (2004) : 157-162.
http://dx.doi.org/10.1002/bdra.20008
---------- MLA ----------
Palermo, A.M., Reynoso, A.S., López Nigro, M., Carballo, M.A., Mudry, M.D. "Teratogenic Evaluation of Metronidazole and Ornidazole Using Drosophila melanogaster as an Experimental Model" . Birth Defects Research Part A - Clinical and Molecular Teratology, vol. 70, no. 4, 2004, pp. 157-162.
http://dx.doi.org/10.1002/bdra.20008
---------- VANCOUVER ----------
Palermo, A.M., Reynoso, A.S., López Nigro, M., Carballo, M.A., Mudry, M.D. Teratogenic Evaluation of Metronidazole and Ornidazole Using Drosophila melanogaster as an Experimental Model. Birth Defects Res. Part A Clin. Mol. Teratol. 2004;70(4):157-162.
http://dx.doi.org/10.1002/bdra.20008