logo biblio digital exactas
logo twitter logo instagram logo whatsapp logo mail

Navegar

Colección

Datos Estadísticas

Artículo

Sigaut, L.; Ponce, M.L.; Colman-Lerner, A.; Dawson, S.P. "Optical techniques provide information on various effective diffusion coefficients in the presence of traps" (2010) Physical Review E - Statistical, Nonlinear, and Soft Matter Physics. 82(5)
La versión final de este artículo es de uso interno. El editor solo permite incluir en el repositorio el artículo en su versión post-print. Por favor, si usted la posee enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

In many cell-signaling pathways information is transmitted via the diffusion of messenger molecules. In most cases, messengers react with other substances and diffuse at the same time. Effective diffusion coefficients may be introduced to characterize the net transport rate that results from the combined effect of these two processes. It was shown in that even in the simplest scenario in which one bimolecular reaction is involved, two different effective coefficients are relevant. One gives the rate at which small perturbations spread out with time while the other relates the mean square displacement of a single particle to the time elapsed. They coincide in the absence of reactions but may be very different in other cases. Optical techniques provide a relatively noninvasive means by which transport rates can be estimated. In the above mentioned paper it was discussed why, under certain conditions, fluorescence recovery after photobleaching (FRAP), a technique commonly used to estimate diffusion rates in cells, provides information on one of the two effective coefficients. In the present paper we show that, under the same conditions, another commonly used optical technique, fluorescence correlation spectroscopy (FCS), gives information on the other one. This opens up the possibility of combining experiments to obtain information that goes beyond effective transport rates. In the present paper we discuss different ways to do so. © 2010 The American Physical Society.

Registro:

Documento: Artículo
Título:Optical techniques provide information on various effective diffusion coefficients in the presence of traps
Autor:Sigaut, L.; Ponce, M.L.; Colman-Lerner, A.; Dawson, S.P.
Filiación:Departamento de Física, FCEN-UBA, Ciudad Universitaria, Pabellón I, (1428) Buenos Aires, Argentina
Instituto de Fisiología, Biología Molecular y Neurociencias, Ciudad Universitaria, Pabellón II, (1428) Buenos Aires, Argentina
Palabras clave:Cell signaling; Fluorescence; Fluorescence spectroscopy; Molecular biology; Optical correlation; Optical engineering; Photobleaching; Spectroscopic analysis; Bimolecular reaction; Effective diffusion coefficients; Fluorescence Correlation Spectroscopy; Fluorescence recovery after photobleaching; Mean square displacement; Net transport rate; Signaling pathways; Small perturbations; Diffusion
Año:2010
Volumen:82
Número:5
DOI: http://dx.doi.org/10.1103/PhysRevE.82.051912
Título revista:Physical Review E - Statistical, Nonlinear, and Soft Matter Physics
Título revista abreviado:Phys. Rev. E Stat. Nonlinear Soft Matter Phys.
ISSN:15393755
CODEN:PLEEE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15393755_v82_n5_p_Sigaut

Referencias:

  • Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P., (2007) Molecular Biology of the Cell, , 5th ed. (Garland Science, Oxford, UK
  • Ellis, R.J., (2001) Curr. Opin. Struct. Biol., 11, p. 114. , 10.1016/S0959-440X(00)00172-X
  • Pando, B., Ponce Dawson, S., Mak, D.D., Pearson, J.E., (2006) Proc. Natl. Acad. Sci. U.S.A., 103, p. 5338. , 10.1073/pnas.0509576103
  • Axelrod, D., Koppel, D.E., Schlessinger, J., Elson, E., Webb, W.W., (1976) Biophys. J., 16, p. 1055. , 10.1016/S0006-3495(76)85755-4
  • Jacobson, K., Wojcieszyn, J., (1984) Proc. Natl. Acad. Sci. U.S.A., 81, p. 6747. , 10.1073/pnas.81.21.6747
  • Gribbon, P., Hardingham, T.E., (1998) Biophys. J., 75, p. 1032. , 10.1016/S0006-3495(98)77592-7
  • Magde, D., Elson, E., Webb, W.W., (1972) Phys. Rev. Lett., 29, p. 705. , 10.1103/PhysRevLett.29.705
  • Elson, E.L., (2001) Traffic, 2, p. 789. , 10.1034/j.1600-0854.2001.21107.x
  • Kim, S.A., Schwille, P., (2003) Curr. Opin. Neurobiol., 13, p. 583. , 10.1016/j.conb.2003.09.002
  • Krichevsky, O., Bonnet, G., (2002) Rep. Prog. Phys., 65, p. 251. , 10.1088/0034-4885/65/2/203
  • Grünwald, D., Cardoso, M.C., Leonhardt, H., Buschmann, V., (2005) Curr. Pharm. Biotechnol., 6, p. 381. , 10.2174/138920105774370616
  • Sprague, B.L., Pego, R.L., Stavreva, D.A., McNally, J.G., (2004) Biophys. J., 86, p. 3473. , 10.1529/biophysj.103.026765
  • Sprague, B.L., McNally, J.G., (2005) Trends Cell Biol., 15, p. 84. , 10.1016/j.tcb.2004.12.001
  • Braeckmans, K., Peeters, L., Sanders, N.N., De Smedt, S.C., Demeester, J., (2003) Biophys. J., 85, p. 2240. , 10.1016/S0006-3495(03)74649-9
  • Stepanenko, O.V., Verkhusha, V.V., Kuznetsova, I.M., Uversky, V.N., Turoverov, K.K., (2008) Curr. Protein Pept. Sci., 9, p. 338. , 10.2174/138920308785132668
  • Chudakov, D., Lukyanov, S., Lukyanov, K., (2005) Trends Biotechnol., 23, p. 605. , 10.1016/j.tibtech.2005.10.005
  • Walling, M.A., Novak, J.A., Shepard, J.R.E., (2009) Int. J. Mol. Sci., 10, p. 441. , 10.3390/ijms10020441
  • Keppler, A., Gendreizig, S., Gronemeyer, T., Pick, H., Vogel, H., Johnsson, K., (2003) Nat. Biotechnol., 21, p. 86. , 10.1038/nbt765
  • Smith, C., (2007) Nat. Methods, 4, p. 755. , 10.1038/nmeth0907-755
  • Bismuto, E., Gratton, E., Lamb, D.C., (2001) Biophys. J., 81, p. 3510. , 10.1016/S0006-3495(01)75982-6
  • Lamb, D.C., Schenk, A., Röcker, C., Scalfi-Happ, C., Ulrich Nienhaus, G., (2000) Biophys. J., 79, p. 1129. , 10.1016/S0006-3495(00)76366-1
  • Meseth, U., Wohland, T., Rigler, R., Vogel, H., (1999) Biophys. J., 76, p. 1619. , 10.1016/S0006-3495(99)77321-2
  • Rein Ten Wolde, P., Tǎnase-Nicola, S., (2006) Nat. Phys., 2, p. 371. , 10.1038/nphys328
  • Abu-Arish, A., Porcher, A., Czerwonka, A., Dostatni, N., Fradin, C., (2010) Biophys. J., 99, p. 33. , 10.1016/j.bpj.2010.05.031
  • Gregor, T., Wieschaus, E.F., McGregor, A.P., Bialek, W., Tank, D.W., (2007) Cell, 130, p. 141. , 10.1016/j.cell.2007.05.026

Citas:

---------- APA ----------
Sigaut, L., Ponce, M.L., Colman-Lerner, A. & Dawson, S.P. (2010) . Optical techniques provide information on various effective diffusion coefficients in the presence of traps. Physical Review E - Statistical, Nonlinear, and Soft Matter Physics, 82(5).
http://dx.doi.org/10.1103/PhysRevE.82.051912
---------- CHICAGO ----------
Sigaut, L., Ponce, M.L., Colman-Lerner, A., Dawson, S.P. "Optical techniques provide information on various effective diffusion coefficients in the presence of traps" . Physical Review E - Statistical, Nonlinear, and Soft Matter Physics 82, no. 5 (2010).
http://dx.doi.org/10.1103/PhysRevE.82.051912
---------- MLA ----------
Sigaut, L., Ponce, M.L., Colman-Lerner, A., Dawson, S.P. "Optical techniques provide information on various effective diffusion coefficients in the presence of traps" . Physical Review E - Statistical, Nonlinear, and Soft Matter Physics, vol. 82, no. 5, 2010.
http://dx.doi.org/10.1103/PhysRevE.82.051912
---------- VANCOUVER ----------
Sigaut, L., Ponce, M.L., Colman-Lerner, A., Dawson, S.P. Optical techniques provide information on various effective diffusion coefficients in the presence of traps. Phys. Rev. E Stat. Nonlinear Soft Matter Phys. 2010;82(5).
http://dx.doi.org/10.1103/PhysRevE.82.051912