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De Siervi, A.; De Luca, P.; Moiola, C.; Gueron, G.; Tongbai, R.; Chandramouli, G.V.R.; Haggerty, C.; Dzekunova, I.; Petersen, D.; Kawasaki, E.; Whoon, J.K.; Camphausen, K.; Longo, D.; Gardner, K. "Identification of new Rel/NFκB regulatory networks by focused genome location analysis" (2009) Cell Cycle. 8(13):2093-2100
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Abstract:

NFκB is an inducible transcription factor that controls kinetically complex patterns of gene expression. Several studies reveal multiple pathways linking NFκB to the promotion and progression of various cancers. Despite extensive interest and characterization, many NFκB controlled genes still remain to be identified. We used chromatin immunoprecipitation combined with microarray technology (ChIP/chip) to investigate the dynamic interaction of NFκB with the promoter regions of 100 genes known to be expressed in mitogen-induced T-cells. Six previously unrecognized NFκB controlled genes (ATM, EP300, TGFβ, Selectin, MMP-1 and SFN) were identified. Each gene is induced in mitogen-stimulated T-cells, repressed by pharmacological NFκB blockade, reduced in cells deficient in the p50 NFκB subunit and dramatically repressed by RNAi specifically designed against cRel. A coregulatory role for Ets transcription factors in the expression of the NFκB controlled genes was predicted by comparative promoter analysis and confirmed by ChIP and by functional disruption of Ets. NFκB deficiency produces a deficit in ATM function and DNA repair indicating an active role for NFκB in maintaining DNA integrity. These results define new potential targets and transcriptional networks governed by NFκB and provide novel functional insights for the role of NFκB in genomic stability, cell cycle control, cell-matrix and cell-cell interactions during tumor progressio. ©2009 Landes Bioscience.

Registro:

Documento: Artículo
Título:Identification of new Rel/NFκB regulatory networks by focused genome location analysis
Autor:De Siervi, A.; De Luca, P.; Moiola, C.; Gueron, G.; Tongbai, R.; Chandramouli, G.V.R.; Haggerty, C.; Dzekunova, I.; Petersen, D.; Kawasaki, E.; Whoon, J.K.; Camphausen, K.; Longo, D.; Gardner, K.
Filiación:National Institutes of Health, Building 41, Bethesda, MD 20892, United States
Microarray Facility, National Cancer Institute, Bethesda, MD, United States
Radiation Oncology Branch, National Cancer Institute, Bethesda, MD, United States
Laboratory of Immunology, National Institute on Aging, Bethesda, MD, United States
School of Sciences, Department of Biological Chemistry, University of Buenos Aires, Intendente Guiraldes 2160, Ciudad de Buenos Aires C1428EGA, Argentina
Palabras clave:ATM; ChIP/chip; Ets; NFκB; T-cells; ATM protein; DNA; E1A associated p300 protein; Ets transcription factor; immunoglobulin enhancer binding protein; interstitial collagenase; mitogenic agent; protein p50; RNA; selectin; transcription factor; transcription factor Rel; transforming growth factor beta; unclassified drug; animal cell; article; cell cycle regulation; cell interaction; chromatin immunoprecipitation; comparative study; controlled study; DNA repair; embryo; extracellular matrix; gene; gene expression; gene identification; gene location; genetic analysis; genome; genomic instability; human; human cell; introspection; microarray analysis; mouse; nonhuman; prediction; promoter region; protein analysis; protein deficiency; protein expression; protein function; protein interaction; RNA interference; SFN gene; T lymphocyte; technology; tumor growth
Año:2009
Volumen:8
Número:13
Página de inicio:2093
Página de fin:2100
DOI: http://dx.doi.org/10.4161/cc.8.13.8926
Título revista:Cell Cycle
Título revista abreviado:Cell Cycle
ISSN:15384101
CAS:DNA, 9007-49-2; interstitial collagenase, 9001-12-1; RNA, 63231-63-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15384101_v8_n13_p2093_DeSiervi

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Citas:

---------- APA ----------
De Siervi, A., De Luca, P., Moiola, C., Gueron, G., Tongbai, R., Chandramouli, G.V.R., Haggerty, C.,..., Gardner, K. (2009) . Identification of new Rel/NFκB regulatory networks by focused genome location analysis. Cell Cycle, 8(13), 2093-2100.
http://dx.doi.org/10.4161/cc.8.13.8926
---------- CHICAGO ----------
De Siervi, A., De Luca, P., Moiola, C., Gueron, G., Tongbai, R., Chandramouli, G.V.R., et al. "Identification of new Rel/NFκB regulatory networks by focused genome location analysis" . Cell Cycle 8, no. 13 (2009) : 2093-2100.
http://dx.doi.org/10.4161/cc.8.13.8926
---------- MLA ----------
De Siervi, A., De Luca, P., Moiola, C., Gueron, G., Tongbai, R., Chandramouli, G.V.R., et al. "Identification of new Rel/NFκB regulatory networks by focused genome location analysis" . Cell Cycle, vol. 8, no. 13, 2009, pp. 2093-2100.
http://dx.doi.org/10.4161/cc.8.13.8926
---------- VANCOUVER ----------
De Siervi, A., De Luca, P., Moiola, C., Gueron, G., Tongbai, R., Chandramouli, G.V.R., et al. Identification of new Rel/NFκB regulatory networks by focused genome location analysis. Cell Cycle. 2009;8(13):2093-2100.
http://dx.doi.org/10.4161/cc.8.13.8926