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Abstract:

Differentiation of Trypanosoma cruzi trypomastigotes to amastigotes inside myoblasts or in vitro, at low extracellular pH, in the presence of [ 3H]palmitic acid or [3H]inositol revealed differential labeling of inositolphosphoceramide and phosphatidylinositol, suggesting that a remodeling process takes place in both lipids. Using 3H-labeled inositolphosphoceramide and phosphatidylinositol as substrates, we demonstrated the association of at least five enzymatic activities with the membranes of amastigotes and trypomastigotes. These included phospholipase A13, phospholipase A2, inositolphosphoceramide-fatty acid hydrolase, acyltransferase, and a phospholipase C releasing either ceramide or a glycerolipid from the inositolphospholipids. These enzymes may be acting in remodeling reactions leading to the anchor of mature glycoproteins or glycoinositolphospholipids and helping in the transformation of the plasma membrane, a necessary step in the differentiation of slender trypomastigotes to round amastigotes, Synthesis of inositolphosphoceramide and particularly of glycoinositolphospholipids was inhibited by aureobasidin A, a known inhibitor of fungal inositolphosphoceramide synthases. The antibiotic impaired the differentiation of trypomastigotes at acidic pH, as indicated by an increased appearance of intermediate forms and a decreased expression of the Ssp4 glycoprotein, a characteristic marker of amastigote forms. Aureobasidin A was also toxic to differentiating trypomastigotes at acidic pH but not to trypomastigotes maintained at neutral pH. Our data suggest that inositolphosphoceramide is implicated in T. cruzi differentiation and that its metabolism could provide important targets for the development of antiparasitic therapies.

Registro:

Documento: Artículo
Título:Formation and remodeling of inositolphosphoceramide during differentiation of Trypanosoma cruzi from trypomastigote to amastigote
Autor:Salto, M.L.; Bertello, L.E.; Vieira, M.; Docampo, R.; Moreno, S.N.J.; De Lederkremer, R.M.
Filiación:Department of Pathobiology, College of Veterinary Medicine, Univ. of IL at Urbana-Champaign, Urbana, IL 61802, United States
CIHIDECAR, Departamento de Quimica Organica, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Palabras clave:aureobasidin A; cyclopeptide; depsipeptide; enzyme inhibitor; glycosphingolipid; inositolphosphoceramides; membrane lipid; membrane protein; phosphatidylinositol; animal; article; cell culture; cell differentiation; cell membrane; cell size; Chagas disease; drug effect; electron microscopy; metabolism; parasitology; pH; physiology; Trypanosoma cruzi; ultrastructure; Animals; Cell Differentiation; Cell Membrane; Cell Size; Cells, Cultured; Chagas Disease; Depsipeptides; Enzyme Inhibitors; Glycosphingolipids; Hydrogen-Ion Concentration; Membrane Glycoproteins; Membrane Lipids; Microscopy, Electron; Peptides, Cyclic; Phosphatidylinositols; Trypanosoma cruzi; Animalia; Eukaryota; Invertebrata; Protozoa; Trypanosoma; Trypanosoma cruzi
Año:2003
Volumen:2
Número:4
Página de inicio:756
Página de fin:768
DOI: http://dx.doi.org/10.1128/EC.2.4.756-768.2003
Título revista:Eukaryotic Cell
Título revista abreviado:Eukaryotic Cell
ISSN:15359778
CODEN:ECUEA
CAS:aureobasidin A, 127785-64-2; aureobasidin A, 127785-64-2; Depsipeptides; Enzyme Inhibitors; Glycosphingolipids; inositolphosphoceramides; Membrane Glycoproteins; Membrane Lipids; Peptides, Cyclic; Phosphatidylinositols
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15359778_v2_n4_p756_Salto

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Citas:

---------- APA ----------
Salto, M.L., Bertello, L.E., Vieira, M., Docampo, R., Moreno, S.N.J. & De Lederkremer, R.M. (2003) . Formation and remodeling of inositolphosphoceramide during differentiation of Trypanosoma cruzi from trypomastigote to amastigote. Eukaryotic Cell, 2(4), 756-768.
http://dx.doi.org/10.1128/EC.2.4.756-768.2003
---------- CHICAGO ----------
Salto, M.L., Bertello, L.E., Vieira, M., Docampo, R., Moreno, S.N.J., De Lederkremer, R.M. "Formation and remodeling of inositolphosphoceramide during differentiation of Trypanosoma cruzi from trypomastigote to amastigote" . Eukaryotic Cell 2, no. 4 (2003) : 756-768.
http://dx.doi.org/10.1128/EC.2.4.756-768.2003
---------- MLA ----------
Salto, M.L., Bertello, L.E., Vieira, M., Docampo, R., Moreno, S.N.J., De Lederkremer, R.M. "Formation and remodeling of inositolphosphoceramide during differentiation of Trypanosoma cruzi from trypomastigote to amastigote" . Eukaryotic Cell, vol. 2, no. 4, 2003, pp. 756-768.
http://dx.doi.org/10.1128/EC.2.4.756-768.2003
---------- VANCOUVER ----------
Salto, M.L., Bertello, L.E., Vieira, M., Docampo, R., Moreno, S.N.J., De Lederkremer, R.M. Formation and remodeling of inositolphosphoceramide during differentiation of Trypanosoma cruzi from trypomastigote to amastigote. Eukaryotic Cell. 2003;2(4):756-768.
http://dx.doi.org/10.1128/EC.2.4.756-768.2003