Artículo

Da Cuña, R.H.; Rey Vázquez, G.; Dorelle, L.; Rodríguez, E.M.; Guimarães Moreira, R.; Lo Nostro, F.L. "Mechanism of action of endosulfan as disruptor of gonadal steroidogenesis in the cichlid fish Cichlasoma dimerus" (2016) Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology. 187:74-80
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Abstract:

The organochlorine pesticide endosulfan (ES) is used in several countries as a wide spectrum insecticide on crops with high commercial value. Due to its high toxicity to non-target animals, its persistence in the environment and its ability to act as an endocrine disrupting compound in fish, ES use is currently banned or restricted in many other countries. Previous studies on the cichlid fish Cichlasoma dimerus have shown that waterborne exposure to ES can lead to both decreased pituitary FSH content and histological alterations of testes. As gonadotropin-stimulated sex steroids release from gonads was inhibited by ES in vitro, the aim of the present study was to elucidate possible mechanisms of disruption of ES on gonadal steroidogenesis in C. dimerus, as well as compare the action of the active ingredient (AI) with that of currently used commercial formulations (CF). Testis and ovary fragments were incubated with ES (AI or CF) and/or steroidogenesis activators or precursors. Testosterone and estradiol levels were measured in the incubation media. By itself, ES did not affect hormone levels. Co-incubation with LH and the adenylate cyclase activator forskolin caused a decrease of the stimulated sex steroids release. When co-incubated with precursors dehydroandrostenedione and 17αhydroxyprogesterone, ES did not affect the increase caused by their addition alone. No differences were observed between the AI and CFs, suggesting that the effect on steroidogenesis disruption is mainly caused by the AI. Results indicate that action of ES takes place downstream of LH-receptor activation and upstream of the studied steroidogenic enzymes.

Registro:

Documento: Artículo
Título:Mechanism of action of endosulfan as disruptor of gonadal steroidogenesis in the cichlid fish Cichlasoma dimerus
Autor:Da Cuña, R.H.; Rey Vázquez, G.; Dorelle, L.; Rodríguez, E.M.; Guimarães Moreira, R.; Lo Nostro, F.L.
Filiación:Laboratorio de Ecotoxicología Acuática, DBBE, FCEyN, Universidad de Buenos Aires, Argentina
Laboratorio de Fisiología de Crustáceos DBBE, FCEyN, Universidad de Buenos Aires, Argentina
Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, Brazil
IBBEA, CONICET-UBA, Buenos Aires, Argentina
Palabras clave:Active ingredients; Commercial formulation; Endocrine disruption; Endosulfan; Gonad culture; Organochlorine pesticide; adenylate cyclase activator; endosulfan; estradiol; forskolin; hydroxyprogesterone; luteinizing hormone; luteinizing hormone receptor; sex hormone; steroid 17alpha monooxygenase; testosterone; adenylate cyclase; endocrine disruptor; endosulfan; enzyme activator; estradiol; forskolin; luteinizing hormone; testosterone; water pollutant; adult; Article; Cichlasoma dimerus; cichlid; comparative study; controlled study; estradiol blood level; female; gonad; hormonal regulation; hormone release; in vitro study; male; nonhuman; priority journal; steroid release; steroidogenesis; testosterone blood level; animal; biosynthesis; cichlid; drug effects; enzyme activation; enzymology; metabolism; ovary; signal transduction; testis; tissue culture technique; toxicity; water pollutant; Adenylyl Cyclases; Animals; Cichlids; Colforsin; Endocrine Disruptors; Endosulfan; Enzyme Activation; Enzyme Activators; Estradiol; Female; Luteinizing Hormone; Male; Ovary; Signal Transduction; Testis; Testosterone; Tissue Culture Techniques; Water Pollutants, Chemical
Año:2016
Volumen:187
Página de inicio:74
Página de fin:80
DOI: http://dx.doi.org/10.1016/j.cbpc.2016.05.008
Título revista:Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
Título revista abreviado:Comp. Biochem. Physiol. C Toxicol. Pharmacol.
ISSN:15320456
CODEN:CBPPF
CAS:endosulfan, 115-29-7; estradiol, 50-28-2; forskolin, 66575-29-9; hydroxyprogesterone, 68-96-2; luteinizing hormone, 39341-83-8, 9002-67-9; steroid 17alpha monooxygenase, 189355-97-3, 9029-67-8, 9044-50-2, 331947-43-4, 9068-00-2; testosterone, 58-22-0; adenylate cyclase, 9012-42-4; Adenylyl Cyclases; Colforsin; Endocrine Disruptors; Endosulfan; Enzyme Activators; Estradiol; Luteinizing Hormone; Testosterone; Water Pollutants, Chemical
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15320456_v187_n_p74_DaCuna

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Citas:

---------- APA ----------
Da Cuña, R.H., Rey Vázquez, G., Dorelle, L., Rodríguez, E.M., Guimarães Moreira, R. & Lo Nostro, F.L. (2016) . Mechanism of action of endosulfan as disruptor of gonadal steroidogenesis in the cichlid fish Cichlasoma dimerus. Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, 187, 74-80.
http://dx.doi.org/10.1016/j.cbpc.2016.05.008
---------- CHICAGO ----------
Da Cuña, R.H., Rey Vázquez, G., Dorelle, L., Rodríguez, E.M., Guimarães Moreira, R., Lo Nostro, F.L. "Mechanism of action of endosulfan as disruptor of gonadal steroidogenesis in the cichlid fish Cichlasoma dimerus" . Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology 187 (2016) : 74-80.
http://dx.doi.org/10.1016/j.cbpc.2016.05.008
---------- MLA ----------
Da Cuña, R.H., Rey Vázquez, G., Dorelle, L., Rodríguez, E.M., Guimarães Moreira, R., Lo Nostro, F.L. "Mechanism of action of endosulfan as disruptor of gonadal steroidogenesis in the cichlid fish Cichlasoma dimerus" . Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, vol. 187, 2016, pp. 74-80.
http://dx.doi.org/10.1016/j.cbpc.2016.05.008
---------- VANCOUVER ----------
Da Cuña, R.H., Rey Vázquez, G., Dorelle, L., Rodríguez, E.M., Guimarães Moreira, R., Lo Nostro, F.L. Mechanism of action of endosulfan as disruptor of gonadal steroidogenesis in the cichlid fish Cichlasoma dimerus. Comp. Biochem. Physiol. C Toxicol. Pharmacol. 2016;187:74-80.
http://dx.doi.org/10.1016/j.cbpc.2016.05.008