Artículo

Poletta, G.L.; Simoniello, M.F.; Mudry, M.D. "Biomarkers of oxidative damage and antioxidant defense capacity in Caiman latirostris blood" (2016) Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology. 179:29-36
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Abstract:

Several xenobiotics, and among them pesticides, can produce oxidative stress, providing a mechanistic basis for their observed toxicity. Chronic oxidative stress induces deleterious modifications to DNA, lipids and proteins that are used as effective biomarkers to study pollutant-mediated oxidative stress. No previous report existed on the application of oxidative damage and antioxidant defense biomarkers in Caiman latirostris blood, while few studies reported in other crocodilians were done in organs or muscles of dead animals. The aim of this study was to characterize a new set of oxidative stress biomarkers in C. latirostris blood, through the modification of conventional techniques: 1) damage to lipids by thiobarbituric acid reactive substances (TBARS), 2) damage to DNA by comet assay modified with the enzymes FPG and Endo III, and 3) antioxidant defenses: catalase, superoxide dismutase and glutathione; in order to apply them in future biomonitoring studies. We successfully adapted standard procedures for CAT, SOD, GSH and TBARS determination in C. latirostris blood. Calibration curves for FPG and Endo III showed that the three dilutions tested were appropriate to conduct the modified comet assay for the detection of oxidized bases in C. latirostris erythrocytes. One hour of incubation allowed a complete repair of the damage generated. The incorporation of these biomarkers in biomonitoring studies of caiman populations exposed to xenobiotics is highly important considering that this species has recovered from a serious endangered state through the implementation of sustainable use programs in Argentina, and represents nowadays a relevant economic resource for many human communities. © 2015 Elsevier Inc.

Registro:

Documento: Artículo
Título:Biomarkers of oxidative damage and antioxidant defense capacity in Caiman latirostris blood
Autor:Poletta, G.L.; Simoniello, M.F.; Mudry, M.D.
Filiación:Cátedra de Toxicología, Farmacología y Bioquímica Legal, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional Del Litoral, Ciudad Universitaria, Paraje el Pozo S/N, Santa Fe, CP 3000, Argentina
Proyecto Yacaré - Laboratorio de Zoología Aplicada: Anexo Vertebrados, Aristóbulo del Valle 8700, Santa Fe, CP 3000, Argentina
Grupo Investigación Biología Evolutiva (GIBE), IEGEBA-DEGE (CONICET-UBA), FCEyN, Pab. II, Ciudad Universitaria, Intendente Güiraldes 2160, Buenos Aires, CP 1428EGA, Argentina
Palabras clave:Biomarkers; Crocodiles; Oxidative stress; Reptiles; Xenobiotics; biological marker; catalase; DNA; glutathione; lipid; superoxide dismutase; thiobarbituric acid reactive substance; xenobiotic agent; antioxidant; biological marker; DNA formamidopyrimidine glycosylase; thiobarbituric acid reactive substance; animal cell; antioxidant assay; Article; biological monitoring; blood analysis; caiman; Caiman latirostris; calibration; comet assay; controlled study; dilution; DNA damage; enzyme assay; erythrocyte; female; lipid peroxidation; male; nonhuman; oxidative stress; priority journal; animal; blood; crocodilian; dose response; metabolism; oxidative stress; physiology; Alligators and Crocodiles; Animals; Antioxidants; Biomarkers; DNA Damage; DNA-Formamidopyrimidine Glycosylase; Dose-Response Relationship, Drug; Erythrocytes; Female; Male; Oxidative Stress; Thiobarbituric Acid Reactive Substances
Año:2016
Volumen:179
Página de inicio:29
Página de fin:36
DOI: http://dx.doi.org/10.1016/j.cbpc.2015.08.003
Título revista:Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
Título revista abreviado:Comp. Biochem. Physiol. C Toxicol. Pharmacol.
ISSN:15320456
CODEN:CBPPF
CAS:catalase, 9001-05-2; DNA, 9007-49-2; glutathione, 70-18-8; lipid, 66455-18-3; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; DNA formamidopyrimidine glycosylase, 78783-53-6; Antioxidants; Biomarkers; DNA-Formamidopyrimidine Glycosylase; Thiobarbituric Acid Reactive Substances
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15320456_v179_n_p29_Poletta

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Citas:

---------- APA ----------
Poletta, G.L., Simoniello, M.F. & Mudry, M.D. (2016) . Biomarkers of oxidative damage and antioxidant defense capacity in Caiman latirostris blood. Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, 179, 29-36.
http://dx.doi.org/10.1016/j.cbpc.2015.08.003
---------- CHICAGO ----------
Poletta, G.L., Simoniello, M.F., Mudry, M.D. "Biomarkers of oxidative damage and antioxidant defense capacity in Caiman latirostris blood" . Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology 179 (2016) : 29-36.
http://dx.doi.org/10.1016/j.cbpc.2015.08.003
---------- MLA ----------
Poletta, G.L., Simoniello, M.F., Mudry, M.D. "Biomarkers of oxidative damage and antioxidant defense capacity in Caiman latirostris blood" . Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, vol. 179, 2016, pp. 29-36.
http://dx.doi.org/10.1016/j.cbpc.2015.08.003
---------- VANCOUVER ----------
Poletta, G.L., Simoniello, M.F., Mudry, M.D. Biomarkers of oxidative damage and antioxidant defense capacity in Caiman latirostris blood. Comp. Biochem. Physiol. C Toxicol. Pharmacol. 2016;179:29-36.
http://dx.doi.org/10.1016/j.cbpc.2015.08.003