Artículo

Rey Vázquez, G.; Meijide, F.J.; Da Cuña, R.H.; Lo Nostro, F.L.; Piazza, Y.G.; Babay, P.A.; Trudeau, V.L.; Maggese, M.C.; Guerrero, G.A. "Exposure to waterborne 4-tert-octylphenol induces vitellogenin synthesis and disrupts testis morphology in the South American freshwater fish Cichlasoma dimerus (Teleostei, Perciformes)" (2009) Comparative Biochemistry and Physiology - C Toxicology and Pharmacology. 150(2):298-306
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Abstract:

Exposure to environmental pollutants may disrupt endocrine functions and cause reproductive effects in human and wildlife populations. Various groups of chemicals have estrogen-like effects, including degradation products of alkylphenol polyethoxylates, such as 4-tert-octylphenol (OP). Laboratory studies have shown that exposure of male fish to xenoestrogens results in induction of circulating vitellogenin (Vtg), inhibition of testicular growth, testis abnormalities and formation of intersex gonads. In this study, the impact of the exposure to waterborne OP on reproductive aspects in the South American cichlid fish Cichlasoma dimerus was evaluated using qualitative changes in the levels of Vtg in plasma and surface mucus and histological alterations in the liver and gonads as endpoints. Adult males and females were exposed to OP via immersion during 60 days in aquaria under semi-static conditions, water changes being made every 84 h. Treatment groups were: control (ethanol 0.005%), OP 30, 150 and 300 μg/L. Using Western and Dot blot analysis, Vtg was detected in plasma and mucus of control and treated females and treated males, while no Vtg was observed in samples from control males. Morphological changes in the hepatocytes due to the accumulation of Vtg were observed in OP-exposed males. Impairment of testicular structure became apparent in males treated with the highest OP concentrations. The most salient pathological change was the alteration of lobular organization with increased testicular fibrosis and progressive disruption of spermatogenesis. No major changes were observed in ovarian architecture. Our results indicate that detection of Vtg in surface mucus may be a sensitive and non-invasive biomarker of the endocrine disrupting effects of environmental estrogens, resulting in a useful method for field monitoring. © 2009 Elsevier Inc. All rights reserved.

Registro:

Documento: Artículo
Título:Exposure to waterborne 4-tert-octylphenol induces vitellogenin synthesis and disrupts testis morphology in the South American freshwater fish Cichlasoma dimerus (Teleostei, Perciformes)
Autor:Rey Vázquez, G.; Meijide, F.J.; Da Cuña, R.H.; Lo Nostro, F.L.; Piazza, Y.G.; Babay, P.A.; Trudeau, V.L.; Maggese, M.C.; Guerrero, G.A.
Filiación:Lab. de Embriología Animal, Dpto. de Biodiversidad y Biología Experimental, FCEyN-UBA, Ciudad Universitaria, Pabellon II, 4 piso, C1428EHA Buenos Aires, Argentina
CONICET, Rivadavia 1917, C1033AAJ Buenos Aires, Argentina
Gerencia de Química, Centro Atómico Constituyentes, CNEA., Av. Gral. Paz 1499, 1650 Pcia. de Buenos Aires, Argentina
Centre for Advanced Research in Environmental Genomics, Dept. of Biology, University of Ottawa, Ottawa, Ont. K1N 6N5, Canada
Palabras clave:Cichlasoma dimerus; Cichlids; Liver and testis pathology; Octylphenol; Surface mucus; Teleosts; Vitellogenin; Xenoestrogens; 4 tert octylphenol; biological marker; endocrine disruptor; estrogen; vitellogenin; animal experiment; animal tissue; article; cell ultrastructure; Cichlasoma dimerus; controlled study; dot hybridization; environmental exposure; female; fibrosis; freshwater fish; immunoblotting; immunohistochemistry; liver cell; male; microscopy; mucus secretion; nonhuman; Perciformes; priority journal; protein blood level; protein induction; spermatogenesis; surface mucus; teleost; testis development; Western blotting; Animals; Argentina; Biological Markers; Dose-Response Relationship, Drug; Endocrine Disruptors; Environmental Monitoring; Female; Fibrosis; Fish Proteins; Fresh Water; Hepatocytes; Liver; Male; Mucus; Perciformes; Phenols; Spermatogenesis; Testis; Time Factors; Vitellogenins; Water Pollutants, Chemical; Cichlasoma; Cichlidae; Perciformes; Teleostei
Año:2009
Volumen:150
Número:2
Página de inicio:298
Página de fin:306
DOI: http://dx.doi.org/10.1016/j.cbpc.2009.05.012
Título revista:Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
Título revista abreviado:Comp. Biochem. Physiol. C Toxicol. Pharmacol.
ISSN:15320456
CODEN:CBPPF
CAS:4 tert octylphenol, 27193-28-8; 4-tert-octylphenol, 140-66-9; Biological Markers; Endocrine Disruptors; Fish Proteins; Phenols; Vitellogenins; Water Pollutants, Chemical
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15320456_v150_n2_p298_ReyVazquez

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Citas:

---------- APA ----------
Rey Vázquez, G., Meijide, F.J., Da Cuña, R.H., Lo Nostro, F.L., Piazza, Y.G., Babay, P.A., Trudeau, V.L.,..., Guerrero, G.A. (2009) . Exposure to waterborne 4-tert-octylphenol induces vitellogenin synthesis and disrupts testis morphology in the South American freshwater fish Cichlasoma dimerus (Teleostei, Perciformes). Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, 150(2), 298-306.
http://dx.doi.org/10.1016/j.cbpc.2009.05.012
---------- CHICAGO ----------
Rey Vázquez, G., Meijide, F.J., Da Cuña, R.H., Lo Nostro, F.L., Piazza, Y.G., Babay, P.A., et al. "Exposure to waterborne 4-tert-octylphenol induces vitellogenin synthesis and disrupts testis morphology in the South American freshwater fish Cichlasoma dimerus (Teleostei, Perciformes)" . Comparative Biochemistry and Physiology - C Toxicology and Pharmacology 150, no. 2 (2009) : 298-306.
http://dx.doi.org/10.1016/j.cbpc.2009.05.012
---------- MLA ----------
Rey Vázquez, G., Meijide, F.J., Da Cuña, R.H., Lo Nostro, F.L., Piazza, Y.G., Babay, P.A., et al. "Exposure to waterborne 4-tert-octylphenol induces vitellogenin synthesis and disrupts testis morphology in the South American freshwater fish Cichlasoma dimerus (Teleostei, Perciformes)" . Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, vol. 150, no. 2, 2009, pp. 298-306.
http://dx.doi.org/10.1016/j.cbpc.2009.05.012
---------- VANCOUVER ----------
Rey Vázquez, G., Meijide, F.J., Da Cuña, R.H., Lo Nostro, F.L., Piazza, Y.G., Babay, P.A., et al. Exposure to waterborne 4-tert-octylphenol induces vitellogenin synthesis and disrupts testis morphology in the South American freshwater fish Cichlasoma dimerus (Teleostei, Perciformes). Comp. Biochem. Physiol. C Toxicol. Pharmacol. 2009;150(2):298-306.
http://dx.doi.org/10.1016/j.cbpc.2009.05.012