Navegar

Colección

Datos Estadísticas

Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

In the majority of Latin-American countries, including Argentina, there is a limited availability of vaginal bioproducts containing probiotics in the market. In addition, the conventional treatments of genital tract infections in women represent a high cost to the public health systems. The future development of this type of bioproducts that employ specific lactobacilli strains would not only have a meaningful impact on women’s health but would also represent a significant challenge to the pharmaceutical industry. The aims of the work described in this paper were (i) to study different pharmaceutical formulations of vaginal ovules containing Lactobacillus fermentum L23 and L. rhamnosus L60, to determine in which formulation lactobacilli viability was sustained for longer time and (ii) to evaluate if probiotic strains maintained both the antimicrobial activity and biofilm-producing ability after being recovered from the ovules. In this study, we developed and characterized three pharmaceutical formulations containing different glycerol amounts and specific lactobacilli strains. Three relevant parameters, cell viability, antimicrobial activity, and biofilm production, by lactobacilli recovered from the ovules were tested. Although the viability of L23 and L60 strains was mainly influenced by high ovule’s glycerol proportion, they survived at 4 °C during the 180 days. Both lactobacilli’s antimicrobial activity and biofilm-producing ability were maintained for all treatments. In conclusion, employing a much reduced number of components, we were able to select the most suitable pharmaceutical formulation which maintained not only lactobacilli viability for a long period of time but also their antimicrobial activity and biofilm-producing ability. © 2017, American Association of Pharmaceutical Scientists.

Registro:

Documento: Artículo
Título:First Steps towards the Pharmaceutical Development of Ovules Containing Lactobacillus Strains: Viability and Antimicrobial Activity as Basic First Parameters in Vaginal Formulations
Autor:Camilletti, A.L.; Ruíz, F.O.; Pascual, L.M.; Barberis, I.L.
Filiación:Area Bacteriología, Departamento de Microbiología e Inmunología, Facultad Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto (UNRC), 5800, Río Cuarto, Córdoba, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Palabras clave:Bioproduct; Lactobacillus; Pharmaceutical formulation; Probiotic; Vaginal ovule; antiinfective agent; glycerol; probiotic agent; antimicrobial activity; Argentina; Article; bacterial strain; biofilm; cell viability; controlled study; drug formulation; drug screening; drug synthesis; Lactobacillus fermentum; Lactobacillus rhamnosus; nonhuman; ovule; priority journal
Año:2018
Volumen:19
Número:2
Página de inicio:886
Página de fin:895
DOI: http://dx.doi.org/10.1208/s12249-017-0895-x
Título revista:AAPS PharmSciTech
Título revista abreviado:AAPS PharmSciTech
ISSN:15309932
CAS:glycerol, 56-81-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15309932_v19_n2_p886_Camilletti

Referencias:

  • Borges, S., Silva, J., Teixeira, P., The role of lactobacilli and probiotics in maintaining vaginal health (2014) Arch Gynecol Obstet, 289, pp. 479-489. , COI: 1:CAS:528:DC%2BC3sXhslWltrjF, PID: 24170161
  • Verdenelli, M.C., Coman, M.M., Cecchini, C., Silvi, S., Orpianesi, C., Cresci, A., Evaluation of antipathogenic activity and adherence properties of human Lactobacillus strains for vaginal formulations (2014) J ApplMicrobiol, 116 (5), pp. 1297-1307. , COI: 1:CAS:528:DC%2BC2cXmt1Wju7Y%3D
  • Kaewsrichan, J., Chandarajoti, K., Kaewnopparat, S., Kaewnopparat, N., Evaluation of lactobacilli containing suppository formulation for probiotic use (2007) MUJPS, 34 (1-4), pp. 1-8
  • Mastromarino, P., Macchia, S., Meggiorini, L., Trinchieri, V., Mosca, L., Perluigi, M., Effectiveness of Lactobacillus-containing vaginal tablets in the treatment of symptomatic bacterial vaginosis. ClinMicrobiol (2008) Infection, 15 (1), pp. 67-74
  • Parma, M., Dindelli, M., Caputo, L., Redaelli, A., Quaranta, L., Candiani, M., The role of vaginal Lactobacillus rhamnosus (Normogin®) in preventing bacterial vaginosis in women with history of recurrences, undergoing surgical menopause: a prospective pilot study (2013) Eur Rev Med Pharmacol Sci, 17 (10), pp. 1399-1403. , COI: 1:STN:280:DC%2BC3sjgtVSnsw%3D%3D, PID: 23740456
  • Juárez Tomás, M.S., De Gregorio, P.R., LecceseTerraf, M.C., Nader-Macías, M.E., Encapsulation and subsequent freeze-drying of Lactobacillus reuteri CRL 1324 for its potential inclusion invaginal probiotic formulations (2015) Eur J Pharm Sci, 79, pp. 87-95. , PID: 26299342
  • Dhewa, T., Pant, S., Mishra, V., Development of freeze dried synbiotic formulation using a probiotic strain of Lactobacillus plantarum (2014) J Food Sci Technol, 51 (1), pp. 83-89. , COI: 1:CAS:528:DC%2BC3sXhvFegtbjM, PID: 24426051
  • Cribby, S., Taylor, M., Reid, G., Vaginal microbiota and the use of probiotics (2008) Inter discip Perspect Infect Dis, pp. 1-9. , https://doi.org/10.1155/2008/256490
  • Ruíz, F.O., (2013) Efecto Terapéutico de las bacteriocinas producidas por las cepas de Lactobacillus fermentum y Lactobacillus rhamnosus sobre microorganismos causales de infecciones genitales [doctoral thesis], , 230 p, Río Cuarto, Universidad Nacional de Río Cuarto, Argentina
  • Rodrigues, F., Maia, M.J., das Neves J, Sarmento B, Amaral MH, Oliveira MB. Vaginal suppositories containing Lactobacillus acidophilus: development and characterization (2015) Drug DevInd Pharm, 41 (9), pp. 1518-1525. , COI: 1:CAS:528:DC%2BC2MXhslWgs7rN
  • Ruíz, F.O., Pascual, L., Giordano, W., Barberis, L., Bacteriocins and other bioactive substances of probiotic lactobacilli as biological weapons against Neisseria gonorrhoeae (2015) PathogDis, 73 (3). , https://doi.org/10.1093/femspd/ftv013
  • Rodríguez, J.M., Probióticos: del laboratorio al consumidor (2015) NutrHosp, 31, pp. 33-47
  • Pascual, L., (2004) Bacteriocinogenia en el género Lactobacillus: características benéficas de lactobacilos de vagina humana [doctoral thesis], , 200 p, Río Cuarto, Universidad Nacional de Río Cuarto, Argentina
  • Pascual, L., Ruíz, F., Giordano, W., Barberis, I.L., Vaginal colonization and activity of the probiotic bacterium Lactobacillus fermentum L23 in a murine model of vaginal tract infection (2010) J Med Microbiol, 59, pp. 360-364. , PID: 19926731
  • Daniele, M., Ruíz, F., Pascual, L., Barberis, L., Ureaplasma urealyticum and Mycoplasma hominis sensitivity to bacteriocins produced by two lactobacilli strains (2011) CurrMicrobiol., 63 (4), pp. 360-365. , COI: 1:CAS:528:DC%2BC3MXhtVyrt73P
  • Daniele, M., Pascual, L., Barberis, L., Curative effect of the probiotic strain Lactobacillus fermentum L23 in a murine model of vaginal infection by Gardnerella vaginalis (2014) LettApplMicrobiol, 59 (1), pp. 93-98. , COI: 1:STN:280:DC%2BC2crkslOmuw%3D%3D
  • Pascual, L., Daniele, M.B., Ruíz, F., Giordano, W., Pájaro, C., Barberis, L., Lactobacillus rhamnosus L60, a potential probiotic isolated from the human vagina (2008) J Gen ApplMicrobiol, 54 (3), pp. 141-148. , COI: 1:CAS:528:DC%2BD1cXotlyrtL0%3D
  • Ortiz, L., Ruiz, F., Pascual, L., Barberis, L., Effect of two probiotic strains of Lactobacillus on in vitro adherence of Listeria monocytogenes, Streptococcus agalactiae, and Staphylococcus aureus to vaginal epithelial cells (2014) CurrMicrobiol., 68 (6), pp. 679-684. , COI: 1:CAS:528:DC%2BC2cXhsV2jurg%3D
  • Pascual, L., Daniele, M.B., Pájaro, C., Barberis, L., Lactobacillus species isolated from the vagina: identification, hydrogen peroxide production and nonoxynol-9resistance (2006) Contraception, 73 (1), pp. 78-81. , COI: 1:CAS:528:DC%2BD2MXhtlGisbjJ, PID: 16371300
  • Ruíz, F.O., Gerbaldo, G., Asurmendi, P., Pascual, L.M., Giordano, W., Barberis, I.L., Antimicrobial activity, inhibition of urogenital pathogens, and synergistic interactions between Lactobacillus strains (2009) CurrMicrobiol., 59 (5), pp. 497-501
  • Pascual, L., Barberis, L., Prevention strategy of urogenital by using Lactobacilli with probiotic properties (2011) Tenke P PhD, pp. 245-264. , In:, editor., Urinary Tract, Infections, Croatia, InTech Europe University Campus STeP Ri, p
  • Ruíz, F.O., Gerbaldo, G., García, M.J., Giordano, W., Pascual, L., Barberis, I.L., Synergistic effect between two bacteriocin-like inhibitory substances produced by lactobacilli strains with inhibitory activity for Streptococcus agalactiae (2012) CurrMicrobiol., 64 (4), pp. 349-356
  • García, M.J., Ruiz, F., Asurmendi, P., Pascual, L., Barberis, L., Bacteriocin producing lactobacilli strains as a biological strategy to control listerial growth (2015) Int J Microbiol Adv Immunol, 3 (2), pp. 60-64
  • Ruiz, F., Pascual, L., Daniele, M.B., Barberis, L., Pájaro, C., Isolation, partial characterization and mode of action of the bacteriocin produced by Lactobacillus rhamnosus (2005) Rev Biocell, 29 (2), p. 235
  • Pascual, L., Daniele, M.B., Giordano, W., Pájaro, M.C., Barberis, I.L., Purification and partial characterization of novel bacteriocin L23 produced by Lactobacillus fermentum L23 (2008) Curr Microbiol, 56 (4), pp. 397-402. , COI: 1:CAS:528:DC%2BD1cXisFCls78%3D, PID: 18172715
  • Ruiz, F., Pascual, L., Daniele, M., Gribaudo, L., Pájaro, M., Barberis, I., Lactobacilli’s bacteriocin interactions on pathogens strains (2007) Higiene Alimentar, 21 (150), pp. 551-552
  • Ruiz, F., Asurmendi, P., Caffaratti, E., Gerbaldo, G., Pascual, L., Barberis, L., Detection of a biologically active substance produced by Lactobacillus fermentum that inhibits Streptococcus agalactiae and Neisseria gonorrhoeae (2009) Rev Biocell, 33 (3), p. 264
  • Lin, W.H., Hwang, C.F., Chen, L.W., Tsen, H.Y., Viable counts, characteristic evaluation for commercial lactic acid bacteria products (2006) Food Microbiol, 23 (1), pp. 74-81. , COI: 1:CAS:528:DC%2BD2MXlsVCksL4%3D, PID: 16942989
  • Saadatzadeh, A., Fazeli, M.R., Jamalifar, H., Dinarvand, R., Probiotic properties of lyophilized cell free extract of Lactobacillus casei (2013) Jundishapur J Nat Pharm Prod, 8 (3), pp. 131-137. , PID: 24624202
  • Siroli, L., Patrignani, F., Serrazanetti, D.I., Parolin, C., Ñahui Palomino, R.A., Vitali, B., Lanciotti, R., Determination of antibacterial and technological properties of vaginal lactobacilli for their potential application in dairy products (2017) Front Microbiol, 8, p. 166. , PID: 28223974
  • de Salud de la Nación, M., Farmacopea Argentina. Argentina: ANMAT 2013; 7th ed Vol I-IV.
  • Kale, V.V., Trivedi, R.V., Wate, S.P., Bhusari, K.P., Development and evaluation of a suppository formulation containing Lactobacillus and its application in vaginal diseases (2005) Ann N Y Acad Sci, 1056, pp. 359-365. , PID: 16387701
  • Terraf, M.C., JuárezTomás, M.S., Nader-Macías, M.E., Silva, C., Screening of biofilm formation by beneficial vaginal lactobacilli and influence of culture media components (2012) J Appl Microbiol, 113 (6), pp. 1517-1529. , PID: 22897406
  • Fernández Ramírez, M.D., Smid, E.J., Abee, T., Nierop Groot, M.N., Characterization of biofilms formed by Lactobacillus plantarum WCFS1 and food spoilage isolates (2015) Int J Food Microbiol, 207, pp. 23-29. , PID: 25965141
  • Vitali, B., Abruzzo, A., Parolin, C., Palomino, R.A., Dalena, F., Bigucci, F., Association of Lactobacillus crispatus with fructo-oligosaccharides and ascorbic acid in hydroxypropylmethylcellulose vaginal insert (2016) Carbohydr Polym, 136, pp. 1161-1169. , COI: 1:CAS:528:DC%2BC2MXhs1Krtb3K, PID: 26572459
  • Maggi, L., Mastromarino, P., Macchia, S., Brigidi, P., Pirovano, F., Matteuzzi, D., Technological and biological evaluation of tablets containing different strains of lactobacilli for vaginal administration (2000) Eur J Pharm Biopharm, 50, pp. 389-395. , COI: 1:CAS:528:DC%2BD3cXovFeis7o%3D, PID: 11072196
  • Zárate, G., Juárez Tomás, M.S., Nader-Macias, M.E., Effect of some pharmaceutical excipients on the survival of probiotic vaginal lactobacilli (2005) Can J Microbiol, 51, pp. 483-489. , PID: 16121226
  • Muller, C., Mazel, V., Dausset, C., Busignies, V., Bornes, S., Nivoliez, A., Study of the Lactobacillus rhamnosus Lcr35® properties after compression and proposition of a model to predict tablet stability (2014) Eur J Pharm Biopharm, 88 (3), pp. 787-794. , COI: 1:CAS:528:DC%2BC2cXhsVSqsbnM, PID: 25128853
  • Albadran, H.A., Chatzifragkou, A., Khutoryanskiy, V.V., Charalampopoulos, D., Stability of probiotic Lactobacillus plantarum in dry microcapsules under accelerated storage conditions (2015) Food Res Int, 74, pp. 208-216. , COI: 1:CAS:528:DC%2BC2MXosVCmu7g%3D, PID: 28411985
  • Vera Pingitore, E., Juárez Tomás, M.S., Wiese, B., Nader-Macías, M.E., Design of novel urogenital pharmabiotic formulations containing lactobacilli, salivaricin CRL 1328 and non-microbial compounds with different functionalities (2015) Drug Dev Ind Pharm, 41 (6), pp. 942-952. , COI: 1:CAS:528:DC%2BC2MXpt1Klt7o%3D, PID: 24827975
  • Uehara, S., Monden, K., Nomoto, K., Seno, Y., Kariyama, R., Kumon, H., A pilot study evaluating the safety and effectiveness of Lactobacillus vaginal suppositories in patients with recurrent urinary tract infection (2006) Int J Antimicrob Agents, 28, pp. S30-S34. , COI: 1:CAS:528:DC%2BD28XotVyktbo%3D, PID: 16859900
  • Kaewnopparat, S., Dangmanee, N., Kaewnopparat, N., Srichana, T., Chulasiri, M., Settharaksa, S., vitro probiotic properties of Lactobacillus fermentum SK5 isolated from vagina of a healthy woman (2013) Anaerobe, 22, pp. 6-13. , COI: 1:CAS:528:DC%2BC3sXns1Kiu7c%3D, PID: 23624069
  • Patterson, J.L., Stull-Lane, A., Girerd, P.H., Jefferson, K.K., Analysis of adherence, biofilm formation and cytotoxicity suggests a greater virulence potential of Gardnerella vaginalis relative to other bacterial-vaginosis-associated anaerobes (2010) Microbiology, 156 (Pt2), pp. 392-399. , COI: 1:CAS:528:DC%2BC3cXivVSls7c%3D, PID: 19910411
  • Murina, F., Graziottin, A., Vicariotto, F., De Seta, F., Can Lactobacillus fermentum LF10 and Lactobacillus acidophilus LA02 in a slow-release vaginal product be useful for prevention of recurrent vulvovaginal candidiasis?: a clinical study (2014) J Clin Gastroenterol, 48, pp. S102-S105. , PID: 25291115
  • Ventolini, G., Mitchell, E., Salazar, M., Biofilm formation by vaginal Lactobacillus in vivo (2015) Med Hypotheses, 84 (5), pp. 417-420. , COI: 1:STN:280:DC%2BC2Mrpslaguw%3D%3D, PID: 25725906
  • Ruíz, F., Gerbaldo, G., Asurmendi, P., García, M., Pascual, L., Barberis, I., Efecto protector de Lactobacillus fermentum L23 en vagina de ratones BALB/c frente a Streptococcus agalactiae (2010) Rev Argent Microbiol, 42 (1), pp. 29-30

Citas:

---------- APA ----------
Camilletti, A.L., Ruíz, F.O., Pascual, L.M. & Barberis, I.L. (2018) . First Steps towards the Pharmaceutical Development of Ovules Containing Lactobacillus Strains: Viability and Antimicrobial Activity as Basic First Parameters in Vaginal Formulations. AAPS PharmSciTech, 19(2), 886-895.
http://dx.doi.org/10.1208/s12249-017-0895-x
---------- CHICAGO ----------
Camilletti, A.L., Ruíz, F.O., Pascual, L.M., Barberis, I.L. "First Steps towards the Pharmaceutical Development of Ovules Containing Lactobacillus Strains: Viability and Antimicrobial Activity as Basic First Parameters in Vaginal Formulations" . AAPS PharmSciTech 19, no. 2 (2018) : 886-895.
http://dx.doi.org/10.1208/s12249-017-0895-x
---------- MLA ----------
Camilletti, A.L., Ruíz, F.O., Pascual, L.M., Barberis, I.L. "First Steps towards the Pharmaceutical Development of Ovules Containing Lactobacillus Strains: Viability and Antimicrobial Activity as Basic First Parameters in Vaginal Formulations" . AAPS PharmSciTech, vol. 19, no. 2, 2018, pp. 886-895.
http://dx.doi.org/10.1208/s12249-017-0895-x
---------- VANCOUVER ----------
Camilletti, A.L., Ruíz, F.O., Pascual, L.M., Barberis, I.L. First Steps towards the Pharmaceutical Development of Ovules Containing Lactobacillus Strains: Viability and Antimicrobial Activity as Basic First Parameters in Vaginal Formulations. AAPS PharmSciTech. 2018;19(2):886-895.
http://dx.doi.org/10.1208/s12249-017-0895-x