Artículo

Bossi, M.; Föiling, J.; Belov, V.N.; Boyarskiy, V.P.; Medda, R.; Egner, A.; Eggeling, C.; Schönle, A.; Hell, S.W. "Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species" (2008) Nano Letters. 8(8):2463-2468
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Abstract:

By combining the photoswitching and localization of individual fluorophores with spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging with low crosstalk and down to 15 nm spatial resolution using only two detection color channels. The applicability of the method to biological specimens is demonstrated on mammalian cells. The combination of far-field fluorescence nanoscopy with the recording of a single switchable molecular species at a time opens up a new class of functional imaging techniques. © 2008 American Chemical Society.

Registro:

Documento: Artículo
Título:Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species
Autor:Bossi, M.; Föiling, J.; Belov, V.N.; Boyarskiy, V.P.; Medda, R.; Egner, A.; Eggeling, C.; Schönle, A.; Hell, S.W.
Filiación:Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany
INQUIMAE-DQIAyQF, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Palabras clave:Imaging techniques; Mammals; Molecular structure; Optical switches; Biological specimens; Color channels; Far fields; Functional imaging; Mammalian cells; Molecular species; Multicolor imaging; New class; Photoswitching; Single-molecule levels; Spatial resolutions; Switchable; Fluorescence; fluorescent dye; animal; article; cell line; chemical structure; chemistry; color; methodology; microscopy; Animals; Cell Line; Color; Fluorescent Dyes; Microscopy; Molecular Structure
Año:2008
Volumen:8
Número:8
Página de inicio:2463
Página de fin:2468
DOI: http://dx.doi.org/10.1021/nl801471d
Handle:http://hdl.handle.net/20.500.12110/paper_15306984_v8_n8_p2463_Bossi
Título revista:Nano Letters
Título revista abreviado:Nano Lett.
ISSN:15306984
CAS:Fluorescent Dyes
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_15306984_v8_n8_p2463_Bossi.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15306984_v8_n8_p2463_Bossi

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Citas:

---------- APA ----------
Bossi, M., Föiling, J., Belov, V.N., Boyarskiy, V.P., Medda, R., Egner, A., Eggeling, C.,..., Hell, S.W. (2008) . Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species. Nano Letters, 8(8), 2463-2468.
http://dx.doi.org/10.1021/nl801471d
---------- CHICAGO ----------
Bossi, M., Föiling, J., Belov, V.N., Boyarskiy, V.P., Medda, R., Egner, A., et al. "Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species" . Nano Letters 8, no. 8 (2008) : 2463-2468.
http://dx.doi.org/10.1021/nl801471d
---------- MLA ----------
Bossi, M., Föiling, J., Belov, V.N., Boyarskiy, V.P., Medda, R., Egner, A., et al. "Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species" . Nano Letters, vol. 8, no. 8, 2008, pp. 2463-2468.
http://dx.doi.org/10.1021/nl801471d
---------- VANCOUVER ----------
Bossi, M., Föiling, J., Belov, V.N., Boyarskiy, V.P., Medda, R., Egner, A., et al. Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species. Nano Lett. 2008;8(8):2463-2468.
http://dx.doi.org/10.1021/nl801471d