Abstract:
By combining the photoswitching and localization of individual fluorophores with spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging with low crosstalk and down to 15 nm spatial resolution using only two detection color channels. The applicability of the method to biological specimens is demonstrated on mammalian cells. The combination of far-field fluorescence nanoscopy with the recording of a single switchable molecular species at a time opens up a new class of functional imaging techniques. © 2008 American Chemical Society.
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Documento: |
Artículo
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Título: | Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species |
Autor: | Bossi, M.; Föiling, J.; Belov, V.N.; Boyarskiy, V.P.; Medda, R.; Egner, A.; Eggeling, C.; Schönle, A.; Hell, S.W. |
Filiación: | Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany INQUIMAE-DQIAyQF, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina
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Palabras clave: | Imaging techniques; Mammals; Molecular structure; Optical switches; Biological specimens; Color channels; Far fields; Functional imaging; Mammalian cells; Molecular species; Multicolor imaging; New class; Photoswitching; Single-molecule levels; Spatial resolutions; Switchable; Fluorescence; fluorescent dye; animal; article; cell line; chemical structure; chemistry; color; methodology; microscopy; Animals; Cell Line; Color; Fluorescent Dyes; Microscopy; Molecular Structure |
Año: | 2008
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Volumen: | 8
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Número: | 8
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Página de inicio: | 2463
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Página de fin: | 2468
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DOI: |
http://dx.doi.org/10.1021/nl801471d |
Título revista: | Nano Letters
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Título revista abreviado: | Nano Lett.
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ISSN: | 15306984
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CAS: | Fluorescent Dyes
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PDF: | https://bibliotecadigital.exactas.uba.ar/download/paper/paper_15306984_v8_n8_p2463_Bossi.pdf |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15306984_v8_n8_p2463_Bossi |
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Citas:
---------- APA ----------
Bossi, M., Föiling, J., Belov, V.N., Boyarskiy, V.P., Medda, R., Egner, A., Eggeling, C.,..., Hell, S.W.
(2008)
. Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species. Nano Letters, 8(8), 2463-2468.
http://dx.doi.org/10.1021/nl801471d---------- CHICAGO ----------
Bossi, M., Föiling, J., Belov, V.N., Boyarskiy, V.P., Medda, R., Egner, A., et al.
"Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species"
. Nano Letters 8, no. 8
(2008) : 2463-2468.
http://dx.doi.org/10.1021/nl801471d---------- MLA ----------
Bossi, M., Föiling, J., Belov, V.N., Boyarskiy, V.P., Medda, R., Egner, A., et al.
"Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species"
. Nano Letters, vol. 8, no. 8, 2008, pp. 2463-2468.
http://dx.doi.org/10.1021/nl801471d---------- VANCOUVER ----------
Bossi, M., Föiling, J., Belov, V.N., Boyarskiy, V.P., Medda, R., Egner, A., et al. Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species. Nano Lett. 2008;8(8):2463-2468.
http://dx.doi.org/10.1021/nl801471d