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Pellegrotti, J.V.; Acuna, G.P.; Puchkova, A.; Holzmeister, P.; Gietl, A.; Lalkens, B.; Stefani, F.D.; Tinnefeld, P. "Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids" (2014) Nano Letters. 14(5):2831-2836
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Abstract:

The amount of information obtainable from a fluorescence-based measurement is limited by photobleaching: Irreversible photochemical reactions either render the molecules nonfluorescent or shift their absorption and/or emission spectra outside the working range. Photobleaching is evidenced as a decrease of fluorescence intensity with time, or in the case of single molecule measurements, as an abrupt, single-step interruption of the fluorescence emission that determines the end of the experiment. Reducing photobleaching is central for improving fluorescence (functional) imaging, single molecule tracking, and fluorescence-based biosensors and assays. In this single molecule study, we use DNA self-assembly to produce hybrid nanostructures containing individual fluorophores and gold nanoparticles at a controlled separation distance of 8.5 nm. By changing the nanoparticles' size we are able to systematically increase the mean number of photons emitted by the fluorophores before photobleaching. © 2014 American Chemical Society.

Registro:

Documento: Artículo
Título:Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids
Autor:Pellegrotti, J.V.; Acuna, G.P.; Puchkova, A.; Holzmeister, P.; Gietl, A.; Lalkens, B.; Stefani, F.D.; Tinnefeld, P.
Filiación:Centro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2390, C1425FQD Buenos Aires, Argentina
Departamento de Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Int. Güiraldes 2620, C1428EAH Ciudad de Buenos Aires, Argentina
NanoBioSciences Group, Institute for Physical and Theoretical Chemistry, Technische Universität Braunschweig, Hans-Sommer-Strasse 10, 38106 Braunschweig, Germany
Palabras clave:DNA self-assembly; gold nanoparticle; photobleaching; plasmonics; single-molecule; DNA; Emission spectroscopy; Fluorescence; Fluorophores; Gold; Metal nanoparticles; Molecules; Self assembly; DNA self-assembly; Fluorescence intensities; Gold Nanoparticles; Plasmonics; Single molecule measurements; Single-molecule; Single-molecule studies; Single-molecule tracking; Photobleaching; DNA; gold; metal nanoparticle; bleaching; chemistry; genetic procedures; photon; Biosensing Techniques; DNA; Gold; Metal Nanoparticles; Photobleaching; Photons
Año:2014
Volumen:14
Número:5
Página de inicio:2831
Página de fin:2836
DOI: http://dx.doi.org/10.1021/nl500841n
Título revista:Nano Letters
Título revista abreviado:Nano Lett.
ISSN:15306984
CODEN:NALEF
CAS:DNA, 9007-49-2; gold, 7440-57-5; DNA; Gold
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15306984_v14_n5_p2831_Pellegrotti

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Citas:

---------- APA ----------
Pellegrotti, J.V., Acuna, G.P., Puchkova, A., Holzmeister, P., Gietl, A., Lalkens, B., Stefani, F.D.,..., Tinnefeld, P. (2014) . Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids. Nano Letters, 14(5), 2831-2836.
http://dx.doi.org/10.1021/nl500841n
---------- CHICAGO ----------
Pellegrotti, J.V., Acuna, G.P., Puchkova, A., Holzmeister, P., Gietl, A., Lalkens, B., et al. "Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids" . Nano Letters 14, no. 5 (2014) : 2831-2836.
http://dx.doi.org/10.1021/nl500841n
---------- MLA ----------
Pellegrotti, J.V., Acuna, G.P., Puchkova, A., Holzmeister, P., Gietl, A., Lalkens, B., et al. "Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids" . Nano Letters, vol. 14, no. 5, 2014, pp. 2831-2836.
http://dx.doi.org/10.1021/nl500841n
---------- VANCOUVER ----------
Pellegrotti, J.V., Acuna, G.P., Puchkova, A., Holzmeister, P., Gietl, A., Lalkens, B., et al. Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids. Nano Lett. 2014;14(5):2831-2836.
http://dx.doi.org/10.1021/nl500841n