Artículo

Sartor, P.A.; Ceballos, L.A.; Orozco, M.M.; Cardinal, M.V.; Gürtler, R.E.; Leguizamón, M.S. "Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species" (2013) Vector-Borne and Zoonotic Diseases. 13(8):581-585
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Abstract:

The detection of Trypanosoma cruzi infection in mammals is crucial for understanding the eco-epidemiological role of the different species involved in parasite transmission cycles. Xenodiagnosis (XD) and hemoculture (HC) are routinely used to detect T. cruzi in wild mammals. Serological methods are much more limited because they require the use of specific antibodies to immunoglobulins of each mammalian species susceptible to T. cruzi. In this study we detected T. cruzi infection by trans-sialidase (TS) inhibition assay (TIA). TIA is based on the antibody neutralization of a recombinant TS that avoids the use of anti-immunoglobulins. TS activity is not detected in the co-endemic protozoan parasites Leishmania spp and T. rangeli. In the current study, serum samples from 158 individuals of nine wild mammalian species, previously tested by XD, were evaluated by TIA. They were collected from two endemic areas in northern Argentina. The overall TIA versus XD co-reactivity was 98.7% (156/158). All 18 samples from XD-positive mammals were TIA-positive (co-positivity, 100%) and co-negativity was 98.5% (138/140). Two XD-negative samples from a marsupial (Didelphis albiventris) and an edentate (Dasypus novemcinctus) were detected by TIA. TIA could be used as a novel tool for serological detection of Trypanosoma cruzi in a wide variety of sylvatic reservoir hosts. © Copyright 2013, Mary Ann Liebert, Inc. 2013.

Registro:

Documento: Artículo
Título:Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species
Autor:Sartor, P.A.; Ceballos, L.A.; Orozco, M.M.; Cardinal, M.V.; Gürtler, R.E.; Leguizamón, M.S.
Filiación:Instituto de Microbiologa y Parasitologa Medica (IMPAM, UBA-CONICET), Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, Piso 13, C1121ABG Buenos Aires, Argentina
Laboratorio de Eco-Epidemiología, Departamento de Ecología, Facultad de Ciencias Exactas y Naturales, Ciudad Autónoma de Buenos Aires, Argentina
Idioma: Inglés
Palabras clave:Chagas disease; Diagnosis; Sylvatic reservoirs; Trans-sialidase; antibody; immunoglobulin; immunoglobulin antibody; sialidase; animal experiment; article; Chagas disease; controlled study; Leishmania; mammal; marsupial; nonhuman; priority journal; protozoon; Trypanosoma cruzi; trypanosomiasis; xenodiagnosis
Año:2013
Volumen:13
Número:8
Página de inicio:581
Página de fin:585
DOI: http://dx.doi.org/10.1089/vbz.2012.1125
Título revista:Vector-Borne and Zoonotic Diseases
Título revista abreviado:Vector Borne Zoonotic Dis.
ISSN:15303667
CODEN:VZDEB
CAS:immunoglobulin, 9007-83-4; sialidase, 9001-67-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15303667_v13_n8_p581_Sartor

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Citas:

---------- APA ----------
Sartor, P.A., Ceballos, L.A., Orozco, M.M., Cardinal, M.V., Gürtler, R.E. & Leguizamón, M.S. (2013) . Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species. Vector-Borne and Zoonotic Diseases, 13(8), 581-585.
http://dx.doi.org/10.1089/vbz.2012.1125
---------- CHICAGO ----------
Sartor, P.A., Ceballos, L.A., Orozco, M.M., Cardinal, M.V., Gürtler, R.E., Leguizamón, M.S. "Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species" . Vector-Borne and Zoonotic Diseases 13, no. 8 (2013) : 581-585.
http://dx.doi.org/10.1089/vbz.2012.1125
---------- MLA ----------
Sartor, P.A., Ceballos, L.A., Orozco, M.M., Cardinal, M.V., Gürtler, R.E., Leguizamón, M.S. "Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species" . Vector-Borne and Zoonotic Diseases, vol. 13, no. 8, 2013, pp. 581-585.
http://dx.doi.org/10.1089/vbz.2012.1125
---------- VANCOUVER ----------
Sartor, P.A., Ceballos, L.A., Orozco, M.M., Cardinal, M.V., Gürtler, R.E., Leguizamón, M.S. Trans-Sialidase Inhibition Assay Detects Trypanosoma cruzi Infection in Different Wild Mammal Species. Vector Borne Zoonotic Dis. 2013;13(8):581-585.
http://dx.doi.org/10.1089/vbz.2012.1125