Artículo
Gelman, D.M.; Noaín, D.; Avale, M.E.; Otero, V.; Low, M.J.; Rubinstein, M. "Transgenic mice engineered to target Cre/LoxP-mediated DNA recombination into catecholaminergic neurons" (2003) Genesis. 36(4):196-202
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Abstract:
To introduce restricted DNA recombination events into catecholaminergic neurons using the Cre/loxP technology, we generated transgenic mice carrying the Cre recombinase gene driven by a 9 kb rat tyrosine hydroxylase (TH) promoter. Immunohistochemistry performed on transgenic mouse brain sections revealed a high number of cells expressing Cre in areas where TH is normally expressed, including the olfactory bulb, hypothalamic and midbrain dopaminergic neurons, and the locus coeruleus. Double immunohistochemistry and immunofluorescence indicated that colocalization of TH and Cre is greater than 80%. Cre expression was also found in TH-positive amacrine neurons of the retina, chromaffin cells of the adrenal medulla, and sympathetic ganglia. We intercrossed TH-Cre mice with the floxed reporter strain Z/AP and observed efficient Cre-mediated recombination in all areas expressing TH, indicating that transgenic Cre is functional. Therefore, we have generated a valuable transgenic mouse strain to induce specific mutations of "floxed" genes in catecholaminergic neurons. © 2003 Wiley-Liss, Inc.
Registro:
| Documento: | Artículo |
| Título: | Transgenic mice engineered to target Cre/LoxP-mediated DNA recombination into catecholaminergic neurons |
| Autor: | Gelman, D.M.; Noaín, D.; Avale, M.E.; Otero, V.; Low, M.J.; Rubinstein, M. |
| Filiación: | Vollum Institute, Dept. of Behavioral Neuroscience, Oregon Health and Science University, Portland, OR, United States Centro de Estudios Cientificos, Valdivia, Chile INGEBI-CONICET, Vuelta de Obligado 2490, 1428-Buenos Aires, Argentina |
| Palabras clave: | Catecholamine; Cre recombinase; Dopamine; LoxP; Norepinephrine; Transgenic mouse; Tyrosine hydroxylase; catecholamine; cre recombinase; dopamine; gene product; protein cre loxp; recombinant DNA; tyrosine 3 monooxygenase; unclassified drug; adrenal medulla; animal cell; article; brain region; catecholamine nerve cell; chromaffin cell; DNA recombination; dopaminergic nerve cell; double immunohistochemistry; floxed gene; gene function; gene mutation; gene targeting; gene technology; genetic engineering; hypothalamus; immunofluorescence; immunohistochemistry; locus ceruleus; mesencephalon; mouse; nonhuman; olfactory bulb; priority journal; promoter region; protein expression; protein localization; rat; retina amacrine cell; strain identification; sympathetic ganglion; transgenic mouse; Alkaline Phosphatase; Animals; Brain Chemistry; Catecholamines; Female; Gene Expression Regulation, Enzymologic; Gene Targeting; Genes, Reporter; Genetic Engineering; Humans; Immunohistochemistry; Integrases; Mice; Mice, Transgenic; Neurons; Pregnancy; Promoter Regions (Genetics); Rats; Recombination, Genetic; Tissue Distribution; Transgenes; Tyrosine 3-Monooxygenase; Viral Proteins; Mus musculus |
| Año: | 2003 |
| Volumen: | 36 |
| Número: | 4 |
| Página de inicio: | 196 |
| Página de fin: | 202 |
| DOI: | http://dx.doi.org/10.1002/gene.10217 |
| Título revista: | Genesis |
| Título revista abreviado: | Genesis |
| ISSN: | 1526954X |
| CODEN: | GNESF |
| CAS: | dopamine, 51-61-6, 62-31-7; tyrosine 3 monooxygenase, 9036-22-0; Alkaline Phosphatase, EC 3.1.3.1; Catecholamines; Cre recombinase, EC 2.7.7.-; Integrases, EC 2.7.7.-; Tyrosine 3-Monooxygenase, EC 1.14.16.2; Viral Proteins |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1526954X_v36_n4_p196_Gelman |
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Citas:
---------- APA ----------
Gelman, D.M., Noaín, D., Avale, M.E., Otero, V., Low, M.J. & Rubinstein, M. (2003) . Transgenic mice engineered to target Cre/LoxP-mediated DNA recombination into catecholaminergic neurons. Genesis, 36(4), 196-202.http://dx.doi.org/10.1002/gene.10217
---------- CHICAGO ----------
Gelman, D.M., Noaín, D., Avale, M.E., Otero, V., Low, M.J., Rubinstein, M. "Transgenic mice engineered to target Cre/LoxP-mediated DNA recombination into catecholaminergic neurons" . Genesis 36, no. 4 (2003) : 196-202.http://dx.doi.org/10.1002/gene.10217
---------- MLA ----------
Gelman, D.M., Noaín, D., Avale, M.E., Otero, V., Low, M.J., Rubinstein, M. "Transgenic mice engineered to target Cre/LoxP-mediated DNA recombination into catecholaminergic neurons" . Genesis, vol. 36, no. 4, 2003, pp. 196-202.http://dx.doi.org/10.1002/gene.10217
---------- VANCOUVER ----------
Gelman, D.M., Noaín, D., Avale, M.E., Otero, V., Low, M.J., Rubinstein, M. Transgenic mice engineered to target Cre/LoxP-mediated DNA recombination into catecholaminergic neurons. Genesis. 2003;36(4):196-202.http://dx.doi.org/10.1002/gene.10217
