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Santagapita, P.R.; Brizuela, L.G.; Mazzobre, M.F.; Ramirez, H.L.; Corti, H.R.; Santana, R.V.; Buera, M.P. "Structure/function relationships of several biopolymers as related to invertase stability in dehydrated systems" (2008) Biomacromolecules. 9(2):741-747
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Abstract:

Structure/function relationships of different biopolymers (alginate, dextran, or β-cyclodextrin) were analyzed as single excipients or combined with trehalose in relation to their efficiency as enzyme stabilizers in freeze-dried formulations and compared to trehalose. Particularly, a novel synthesized polymer β-cyclodextrin-branched alginate (β-CD-A) was employed as excipient. During freeze-drying, the polymers or their mixtures did not confer better protection to invertase compared to trehalose. β-CD-A (with or without trehalose), β-cyclodextrin (β-CD), or dextran with trehalose were the best protective agents during thermal treatment, while β-CD and algiate showed a negative effect on invertase activity preservation. The β-CD linked alginate combined the physical stability provided by alginate with the stabilization of hydrophobic regions of the enzyme provided by cyclodextrin. β-CD-A was effective even at conditions at which trehalose lost its protective effect. A relatively simple covalent combination of two biopolymers significantly affected their functionalities and, consequently, their interactions with proteins, modifying enzyme stability patterns. © 2008 American Chemical Society.

Registro:

Documento: Artículo
Título:Structure/function relationships of several biopolymers as related to invertase stability in dehydrated systems
Autor:Santagapita, P.R.; Brizuela, L.G.; Mazzobre, M.F.; Ramirez, H.L.; Corti, H.R.; Santana, R.V.; Buera, M.P.
Filiación:Departamentos de Industrias y de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Centro de Estudios de Tecnología Enzimática, Facultad de Agronomía, Universidad de Matanzas Camilo Cienfuegos, Autopista a Varadero km 3 1/2, 44740 Matanzas, Cuba
Comisión Nacional de Energía Atómica, Centro Atómico Constituyentes, Av. Constituyentes, San Martín, Buenos Aires, Argentina
Departamento de Quimica Inorganica Analitica y Quimica Fisica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Palabras clave:Alginate; Dextran; Enzymes; Mixtures; Polysaccharides; Dehydrated systems; Invertase stability; Trehalose; Biopolymers; alginic acid; beta cyclodextrin; beta fructofuranosidase; biopolymer; dextran; excipient; polymer; protective agent; trehalose; article; chemical analysis; chemical binding; chemical modification; chemical structure; controlled study; covalent bond; dehydration; enzyme stability; freeze drying; hydrophobicity; priority journal; protein interaction; synthesis; thermostability; beta-Fructofuranosidase; Desiccation; Drug Stability; Enzyme Stability; Freeze Drying; Laminaria; Polymers; Structure-Activity Relationship
Año:2008
Volumen:9
Número:2
Página de inicio:741
Página de fin:747
DOI: http://dx.doi.org/10.1021/bm7012108
Título revista:Biomacromolecules
Título revista abreviado:Biomacromolecules
ISSN:15257797
CODEN:BOMAF
CAS:alginic acid, 28961-37-7, 29894-36-8, 9005-32-7, 9005-38-3; beta cyclodextrin, 7585-39-9; beta fructofuranosidase, 9001-57-4; dextran, 87915-38-6, 9014-78-2; trehalose, 99-20-7; beta-Fructofuranosidase, EC 3.2.1.26; Polymers
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15257797_v9_n2_p741_Santagapita

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Citas:

---------- APA ----------
Santagapita, P.R., Brizuela, L.G., Mazzobre, M.F., Ramirez, H.L., Corti, H.R., Santana, R.V. & Buera, M.P. (2008) . Structure/function relationships of several biopolymers as related to invertase stability in dehydrated systems. Biomacromolecules, 9(2), 741-747.
http://dx.doi.org/10.1021/bm7012108
---------- CHICAGO ----------
Santagapita, P.R., Brizuela, L.G., Mazzobre, M.F., Ramirez, H.L., Corti, H.R., Santana, R.V., et al. "Structure/function relationships of several biopolymers as related to invertase stability in dehydrated systems" . Biomacromolecules 9, no. 2 (2008) : 741-747.
http://dx.doi.org/10.1021/bm7012108
---------- MLA ----------
Santagapita, P.R., Brizuela, L.G., Mazzobre, M.F., Ramirez, H.L., Corti, H.R., Santana, R.V., et al. "Structure/function relationships of several biopolymers as related to invertase stability in dehydrated systems" . Biomacromolecules, vol. 9, no. 2, 2008, pp. 741-747.
http://dx.doi.org/10.1021/bm7012108
---------- VANCOUVER ----------
Santagapita, P.R., Brizuela, L.G., Mazzobre, M.F., Ramirez, H.L., Corti, H.R., Santana, R.V., et al. Structure/function relationships of several biopolymers as related to invertase stability in dehydrated systems. Biomacromolecules. 2008;9(2):741-747.
http://dx.doi.org/10.1021/bm7012108