Artículo

Taranda, J.; Ballestero, J.A.; Hiel, H.; De Souza, F.S.J.; Wedemeyer, C.; Gómez-Casati, M.E.; Lipovsek, M.; Vetter, D.E.; Fuchs, P.A.; Katz, E.; Elgoyhen, A.B. "Constitutive expression of the α10 nicotinic acetylcholine receptor subunit fails to maintain cholinergic responses in inner hair cells after the onset of hearing" (2009) JARO - Journal of the Association for Research in Otolaryngology. 10(3):397-406
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Abstract:

Efferent inhibition of cochlear hair cells is mediated by α9α10 nicotinic cholinergic receptors (nAChRs) functionally coupled to calcium-activated, small conductance (SK2) potassium channels. Before the onset of hearing, efferent fibers transiently make functional cholinergic synapses with inner hair cells (IHCs). The retraction of these fibers after the onset of hearing correlates with the cessation of transcription of the Chrna10 (but not the Chrna9) gene in IHCs. To further analyze this developmental change, we generated a transgenic mice whose IHCs constitutively express α10 into adulthood by expressing the α10 cDNA under the control of the Pou4f3 gene promoter. In situ hybridization showed that the α10 mRNA is expressed in IHCs of 8-week-old transgenic mice, but not in wild-type mice. Moreover, this mRNA is translated into a functional protein, since IHCs from P8-P10 α10 transgenic mice backcrossed to a Chrna10 -/- background (whose IHCs have no cholinergic function) displayed normal synaptic and acetylcholine (ACh)-evoked currents in patch-clamp recordings. Thus, the α10 transgene restored nAChR function. However, in the α10 transgenic mice, no synaptic or ACh-evoked currents were observed in P16-18 IHCs, indicating developmental down-regulation of functional nAChRs after the onset of hearing, as normally observed in wild-type mice. The lack of functional ACh currents correlated with the lack of SK2 currents. These results indicate that multiple features of the efferent postsynaptic complex to IHCs, in addition to the nAChR subunits, are down-regulated in synchrony after the onset of hearing, leading to lack of responses to ACh. © 2009 Association for Research in Otolaryngology.

Registro:

Documento: Artículo
Título:Constitutive expression of the α10 nicotinic acetylcholine receptor subunit fails to maintain cholinergic responses in inner hair cells after the onset of hearing
Autor:Taranda, J.; Ballestero, J.A.; Hiel, H.; De Souza, F.S.J.; Wedemeyer, C.; Gómez-Casati, M.E.; Lipovsek, M.; Vetter, D.E.; Fuchs, P.A.; Katz, E.; Elgoyhen, A.B.
Filiación:Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires 1428, Argentina
Department of Otolaryngology, Head and Neck Surgery, School of Medicine, Johns Hopkins University, Baltimore, MD 21205-2195, United States
Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires 1428, Argentina
Deptartment of Neuroscience, Tufts University, School of Medicine, Boston, MA 02111, United States
Departamento de Farmacología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires 1121, Argentina
Palabras clave:Acetylcholine; Efferent medial olivocochlear; Nicotinic cholinergic receptors; SK2 channel; Transgenic mice; acetylcholine; complementary DNA; messenger RNA; nicotinic acetylcholine receptor alpha10; nicotinic receptor; potassium channel SK2; receptor subunit; small conductance calcium activated potassium channel; unclassified drug; acetylcholine; cholinergic receptor stimulating agent; Chrna10 protein, mouse; homeodomain protein; Kcnn2 protein, mouse; messenger RNA; nicotinic receptor; Pou4f3 protein, mouse; small conductance calcium activated potassium channel; transcription factor POU4F3; animal experiment; animal tissue; article; cholinergic synapse; Chrna10 gene; controlled study; down regulation; efferent nerve; evoked response; gene expression; genetic transcription; hair cell; hearing; in situ hybridization; mouse; nonhuman; patch clamp; potassium current; Pou4f3 gene; priority journal; promoter region; receptor gene; RNA translation; transgene; transgenic mouse; wild type; animal; animal model; cytology; drug effect; genetics; metabolism; physiology; Acetylcholine; Animals; Cholinergic Agents; Hair Cells, Auditory, Inner; Hearing; Homeodomain Proteins; Mice; Mice, Transgenic; Models, Animal; Patch-Clamp Techniques; Receptors, Nicotinic; RNA, Messenger; Small-Conductance Calcium-Activated Potassium Channels; Transcription Factor Brn-3C
Año:2009
Volumen:10
Número:3
Página de inicio:397
Página de fin:406
DOI: http://dx.doi.org/10.1007/s10162-009-0173-z
Título revista:JARO - Journal of the Association for Research in Otolaryngology
Título revista abreviado:JARO J. Assoc. Res. Otolaryngol.
ISSN:15253961
CODEN:JJARB
CAS:acetylcholine, 51-84-3, 60-31-1, 66-23-9; Acetylcholine, 51-84-3; Cholinergic Agents; Chrna10 protein, mouse; Homeodomain Proteins; Kcnn2 protein, mouse; Pou4f3 protein, mouse; RNA, Messenger; Receptors, Nicotinic; Small-Conductance Calcium-Activated Potassium Channels; Transcription Factor Brn-3C
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_15253961_v10_n3_p397_Taranda.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15253961_v10_n3_p397_Taranda

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Citas:

---------- APA ----------
Taranda, J., Ballestero, J.A., Hiel, H., De Souza, F.S.J., Wedemeyer, C., Gómez-Casati, M.E., Lipovsek, M.,..., Elgoyhen, A.B. (2009) . Constitutive expression of the α10 nicotinic acetylcholine receptor subunit fails to maintain cholinergic responses in inner hair cells after the onset of hearing. JARO - Journal of the Association for Research in Otolaryngology, 10(3), 397-406.
http://dx.doi.org/10.1007/s10162-009-0173-z
---------- CHICAGO ----------
Taranda, J., Ballestero, J.A., Hiel, H., De Souza, F.S.J., Wedemeyer, C., Gómez-Casati, M.E., et al. "Constitutive expression of the α10 nicotinic acetylcholine receptor subunit fails to maintain cholinergic responses in inner hair cells after the onset of hearing" . JARO - Journal of the Association for Research in Otolaryngology 10, no. 3 (2009) : 397-406.
http://dx.doi.org/10.1007/s10162-009-0173-z
---------- MLA ----------
Taranda, J., Ballestero, J.A., Hiel, H., De Souza, F.S.J., Wedemeyer, C., Gómez-Casati, M.E., et al. "Constitutive expression of the α10 nicotinic acetylcholine receptor subunit fails to maintain cholinergic responses in inner hair cells after the onset of hearing" . JARO - Journal of the Association for Research in Otolaryngology, vol. 10, no. 3, 2009, pp. 397-406.
http://dx.doi.org/10.1007/s10162-009-0173-z
---------- VANCOUVER ----------
Taranda, J., Ballestero, J.A., Hiel, H., De Souza, F.S.J., Wedemeyer, C., Gómez-Casati, M.E., et al. Constitutive expression of the α10 nicotinic acetylcholine receptor subunit fails to maintain cholinergic responses in inner hair cells after the onset of hearing. JARO J. Assoc. Res. Otolaryngol. 2009;10(3):397-406.
http://dx.doi.org/10.1007/s10162-009-0173-z