Artículo

Gómez-Casati, M.E.; Wedemeyer, C.; Taranda, J.; Lipovsek, M.; Dalamon, V.; Elgoyhen, A.B.; Katz, E. "Electrical properties and functional expression of ionic channels in cochlear inner hair cells of mice lacking the α10 nicotinic cholinergic receptor subunit" (2009) JARO - Journal of the Association for Research in Otolaryngology. 10(2):221-232
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Abstract:

Cochlear inner hair cells (IHCs) release neurotransmitter onto afferent auditory nerve fibers in response to sound stimulation. During early development, synaptic transmission is triggered by spontaneous Ca2+ spikes which are modulated by an efferent cholinergic innervation to IHCs. This synapse is inhibitory and mediated by the α9α10 nicotinic cholinergic receptor (nAChR). After the onset of hearing, large-conductance Ca2+-activated K+ channels are acquired and both the spiking activity and the efferent innervation disappear from IHCs. In this work, we studied the developmental changes in the membrane properties of cochlear IHCs from α10 nAChR gene (Chrna10) "knockout" mice. Electrophysiological properties of IHCs were studied by whole-cell recordings in acutely excised apical turns of the organ of Corti from developing mice. Neither the spiking activity nor the developmental functional expression of voltage-gated and/or calcium-sensitive K+ channels is altered in the absence of the α10 nAChR subunit. The present results show that the α10 nAChR subunit is not essential for the correct establishment of the intrinsic electrical properties of IHCs during development. © 2009 Association for Research in Otolaryngology.

Registro:

Documento: Artículo
Título:Electrical properties and functional expression of ionic channels in cochlear inner hair cells of mice lacking the α10 nicotinic cholinergic receptor subunit
Autor:Gómez-Casati, M.E.; Wedemeyer, C.; Taranda, J.; Lipovsek, M.; Dalamon, V.; Elgoyhen, A.B.; Katz, E.
Filiación:Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas, Vuelta de Obligado 2490, 1428, Buenos Aires, Argentina
Departamento de Farmacología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Auditory; Calcium spikes; Calcium-sensitive K+ channels; Cholinergic; Development; Voltage-gated K+ channels; calcium; calcium activated potassium channel; calcium channel; cholinergic receptor; neurotransmitter; nicotinic receptor; potassium channel; voltage gated potassium channel; acoustic nerve fiber; animal cell; article; auditory stimulation; calcium conductance; calcium transport; Chrna10 gene; cochlea; controlled study; Corti organ; electric potential; electrophysiology; gene; hair cell; hearing; mouse; neurotransmitter release; newborn; nonhuman; parasympathetic innervation; priority journal; sensory nerve; synapse; synaptic transmission; whole cell; Animals; Apamin; Cochlea; Electric Capacitance; Hair Cells, Auditory, Inner; Hearing; Mice; Patch-Clamp Techniques; Potassium Channels, Calcium-Activated; Receptors, Nicotinic
Año:2009
Volumen:10
Número:2
Página de inicio:221
Página de fin:232
DOI: http://dx.doi.org/10.1007/s10162-009-0164-0
Título revista:JARO - Journal of the Association for Research in Otolaryngology
Título revista abreviado:JARO J. Assoc. Res. Otolaryngol.
ISSN:15253961
CODEN:JJARB
CAS:calcium, 7440-70-2; Apamin, 24345-16-2; Chrna10 protein, mouse; Potassium Channels, Calcium-Activated; Receptors, Nicotinic
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15253961_v10_n2_p221_GomezCasati

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Citas:

---------- APA ----------
Gómez-Casati, M.E., Wedemeyer, C., Taranda, J., Lipovsek, M., Dalamon, V., Elgoyhen, A.B. & Katz, E. (2009) . Electrical properties and functional expression of ionic channels in cochlear inner hair cells of mice lacking the α10 nicotinic cholinergic receptor subunit. JARO - Journal of the Association for Research in Otolaryngology, 10(2), 221-232.
http://dx.doi.org/10.1007/s10162-009-0164-0
---------- CHICAGO ----------
Gómez-Casati, M.E., Wedemeyer, C., Taranda, J., Lipovsek, M., Dalamon, V., Elgoyhen, A.B., et al. "Electrical properties and functional expression of ionic channels in cochlear inner hair cells of mice lacking the α10 nicotinic cholinergic receptor subunit" . JARO - Journal of the Association for Research in Otolaryngology 10, no. 2 (2009) : 221-232.
http://dx.doi.org/10.1007/s10162-009-0164-0
---------- MLA ----------
Gómez-Casati, M.E., Wedemeyer, C., Taranda, J., Lipovsek, M., Dalamon, V., Elgoyhen, A.B., et al. "Electrical properties and functional expression of ionic channels in cochlear inner hair cells of mice lacking the α10 nicotinic cholinergic receptor subunit" . JARO - Journal of the Association for Research in Otolaryngology, vol. 10, no. 2, 2009, pp. 221-232.
http://dx.doi.org/10.1007/s10162-009-0164-0
---------- VANCOUVER ----------
Gómez-Casati, M.E., Wedemeyer, C., Taranda, J., Lipovsek, M., Dalamon, V., Elgoyhen, A.B., et al. Electrical properties and functional expression of ionic channels in cochlear inner hair cells of mice lacking the α10 nicotinic cholinergic receptor subunit. JARO J. Assoc. Res. Otolaryngol. 2009;10(2):221-232.
http://dx.doi.org/10.1007/s10162-009-0164-0