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Abstract:

Solid-state fermentation (SSF) with the medicinal higher Basidiomycete Ganoderma lucidum was studied as a strategy to use pine (Pinus radiata D. Don) and poplar (Populus nigra L.) wood chips and sawdust. Fruiting bodies were produced and the value of the biotransformed substrate was assessed. The highest mushroom yield (63 g dry weight per kilogram of dry substrate) was obtained with poplar sawdust and wood chips. Immersion of the bioreactors was a simple watering method that obtained suitable yields. Two morphological types were induced using 2 different incandescent light intensities. High light irradiation induced the highest valued mushroom morphology (as a whole product). Time course study of substrate biodegradation and mycelial growth dynamics indicated that the trophophase lasted 20 days and presented laccase activity of 0.01–0.03 units · g−1. The activity at idiophase was 10 times higher. Aqueous and alkali extracts, as well as carbohydrase enzyme profile activity, revealed differences in the properties of the residual substrate; some related to the substrate source are considered to be of concern for further use of this pretreated biomass. In view of the results obtained, we propose use of SSF of pine and poplar with G. lucidum to profitably recycle softwood by-products from the timber industry. © 2018 Begell House, Inc.

Registro:

Documento: Artículo
Título:Conditions affecting lingzhi or reishi medicinal mushroom ganoderma lucidum (Agaricomycetes) basidiome quality, morphogenesis, and biodegradation of wood by-products in Argentina
Autor:Kuhar, F.; Postemsky, P.D.; Bianchinotti, M.V.
Filiación:Instituto de Micología y Botánica, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Laboratorio de Micología Experimental, Ciudad Autónoma de Buenos Aires, Argentina
Centro de Recursos Renovables de la Zona Semiárida (CERZOS), Universidad Nacional del Sur (UNS), CONICET, Laboratorio de Biotecnología de Hongos Comestibles y Medicinales, Bahía Blanca, Buenos Aires, Argentina
CERZOS, UNS, CONICET, Laboratorio de Micología, Fitopatología, y Control Biológico, Bahía Blanca, Buenos Aires, Argentina
Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur, Bahía Blanca, Buenos Aires, Argentina
Instituto Multidisciplinario de Biología Vegetal, Universidad Nacional de Córdoba, CONICET, Avenida Vélez Sársfield 161, Córdoba, 5000, Argentina
Palabras clave:Fungal morphogenesis; Ganoderma lucidum; Laccase; Lignocellulolytic enzymes; Medicinal mushrooms; Solid-state fermentation; alkali; fungal enzyme; Ganoderma lucidum extract; idiophase; laccase; trophophase; unclassified drug; laccase; lignin; lignocellulose; aqueous solution; Argentina; Article; biodegradation; controlled study; dry weight; enzyme activity; fruiting body; fungal biomass; fungal structures; Ganoderma lucidum; immersion; irradiation; light intensity; morphogenesis; mycelial growth; nonhuman; pine; Pinus radiata; Populus nigra; sawdust; solid state fermentation; wood chip; biomass; bioreactor; chemistry; fermentation; Ganoderma lucidum; growth, development and aging; light; metabolism; microbiology; morphogenesis; mycelium; Populus; radiation response; wood; Argentina; Biomass; Bioreactors; Fermentation; Fruiting Bodies, Fungal; Laccase; Light; Lignin; Morphogenesis; Mycelium; Pinus; Populus; Reishi; Wood
Año:2018
Volumen:20
Número:5
Página de inicio:495
Página de fin:506
DOI: http://dx.doi.org/10.1615/IntJMedMushrooms.2018026249
Título revista:International Journal of Medicinal Mushrooms
Título revista abreviado:Int. J. Med. Mushrooms
ISSN:15219437
CODEN:IMMUF
CAS:laccase, 80498-15-3; lignin, 9005-53-2; lignocellulose, 11132-73-3; Laccase; Lignin; lignocellulose
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15219437_v20_n5_p495_Kuhar

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Citas:

---------- APA ----------
Kuhar, F., Postemsky, P.D. & Bianchinotti, M.V. (2018) . Conditions affecting lingzhi or reishi medicinal mushroom ganoderma lucidum (Agaricomycetes) basidiome quality, morphogenesis, and biodegradation of wood by-products in Argentina. International Journal of Medicinal Mushrooms, 20(5), 495-506.
http://dx.doi.org/10.1615/IntJMedMushrooms.2018026249
---------- CHICAGO ----------
Kuhar, F., Postemsky, P.D., Bianchinotti, M.V. "Conditions affecting lingzhi or reishi medicinal mushroom ganoderma lucidum (Agaricomycetes) basidiome quality, morphogenesis, and biodegradation of wood by-products in Argentina" . International Journal of Medicinal Mushrooms 20, no. 5 (2018) : 495-506.
http://dx.doi.org/10.1615/IntJMedMushrooms.2018026249
---------- MLA ----------
Kuhar, F., Postemsky, P.D., Bianchinotti, M.V. "Conditions affecting lingzhi or reishi medicinal mushroom ganoderma lucidum (Agaricomycetes) basidiome quality, morphogenesis, and biodegradation of wood by-products in Argentina" . International Journal of Medicinal Mushrooms, vol. 20, no. 5, 2018, pp. 495-506.
http://dx.doi.org/10.1615/IntJMedMushrooms.2018026249
---------- VANCOUVER ----------
Kuhar, F., Postemsky, P.D., Bianchinotti, M.V. Conditions affecting lingzhi or reishi medicinal mushroom ganoderma lucidum (Agaricomycetes) basidiome quality, morphogenesis, and biodegradation of wood by-products in Argentina. Int. J. Med. Mushrooms. 2018;20(5):495-506.
http://dx.doi.org/10.1615/IntJMedMushrooms.2018026249