Artículo

Boron, I.; Russo, R.; Boechi, L.; Cheng, C.-H.C.; Di Prisco, G.; Estrin, D.A.; Verde, C.; Nadra, A.D. "Structure and dynamics of Antarctic fish neuroglobin assessed by computer simulations" (2011) IUBMB Life. 63(3):206-213
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Abstract:

Neuroglobin (Ngb) is a heme protein, highly conserved along evolution, predominantly found in the nervous system. It is upregulated by hypoxia and ischemia and may have a neuroprotective role under hypoxic stress. Although many other roles have been proposed, the physiological function is still unclear. Antarctic icefishes lack hemoglobin and some species also lack myoglobin, but all have Ngb and thus may help the elucidation of Ngb function. We present the first theoretically derived structure of fish Ngb and describe its behavior using molecular dynamics simulations. Specifically, we sequenced and analyzed Ngbs from a colorless-blooded Antarctic icefish species Chaenocephalus aceratus and a related red-blooded species (Dissostichus mawsoni). Both fish Ngbs are 6-coordinated but have some peculiarities that differentiate them from mammalian counterparts: they have extensions in the N and C termini that can interact with the EF loop, and a gap in the alignment that changes the CD-region structure/dynamics that has been found to play a key role in human neuroglobin. Our results suggest that a single mutation between both fish Ngbs is responsible for significant difference in the behavior of the proteins. The functional role of these characteristics is discussed. © 2011 IUBMB.

Registro:

Documento: Artículo
Título:Structure and dynamics of Antarctic fish neuroglobin assessed by computer simulations
Autor:Boron, I.; Russo, R.; Boechi, L.; Cheng, C.-H.C.; Di Prisco, G.; Estrin, D.A.; Verde, C.; Nadra, A.D.
Filiación:Departamento de Química Biol0́gica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina
Departamento de Química Inorgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina
Institute of Protein Biochemistry, CNR, Via Pietro Castellino 111, I-80131 Naples, Italy
Department of Animal Biology, University of Illinois, Urbana, IL, United States
Departamento de Fisiología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina
Palabras clave:evolution; hemeproteins; neuroglobin; protein function; protein structure; structural biology; neuroglobin; amino terminal sequence; Antarctica; carboxy terminal sequence; computer simulation; conference paper; fish; gene sequence; molecular dynamics; mutation; nonhuman; nucleotide sequence; protein analysis; protein function; protein structure; Amino Acid Sequence; Animals; Antarctic Regions; Base Sequence; Computer Simulation; DNA Primers; Fishes; Globins; Molecular Dynamics Simulation; Molecular Sequence Data; Nerve Tissue Proteins; Sequence Homology, Amino Acid; Chaenocephalus aceratus; Dissostichus mawsoni; Mammalia; Salangini
Año:2011
Volumen:63
Número:3
Página de inicio:206
Página de fin:213
DOI: http://dx.doi.org/10.1002/iub.444
Título revista:IUBMB Life
Título revista abreviado:IUBMB Life
ISSN:15216543
CODEN:IULIF
CAS:DNA Primers; Globins, 9004-22-2; Nerve Tissue Proteins; neuroglobin
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15216543_v63_n3_p206_Boron

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Citas:

---------- APA ----------
Boron, I., Russo, R., Boechi, L., Cheng, C.-H.C., Di Prisco, G., Estrin, D.A., Verde, C.,..., Nadra, A.D. (2011) . Structure and dynamics of Antarctic fish neuroglobin assessed by computer simulations. IUBMB Life, 63(3), 206-213.
http://dx.doi.org/10.1002/iub.444
---------- CHICAGO ----------
Boron, I., Russo, R., Boechi, L., Cheng, C.-H.C., Di Prisco, G., Estrin, D.A., et al. "Structure and dynamics of Antarctic fish neuroglobin assessed by computer simulations" . IUBMB Life 63, no. 3 (2011) : 206-213.
http://dx.doi.org/10.1002/iub.444
---------- MLA ----------
Boron, I., Russo, R., Boechi, L., Cheng, C.-H.C., Di Prisco, G., Estrin, D.A., et al. "Structure and dynamics of Antarctic fish neuroglobin assessed by computer simulations" . IUBMB Life, vol. 63, no. 3, 2011, pp. 206-213.
http://dx.doi.org/10.1002/iub.444
---------- VANCOUVER ----------
Boron, I., Russo, R., Boechi, L., Cheng, C.-H.C., Di Prisco, G., Estrin, D.A., et al. Structure and dynamics of Antarctic fish neuroglobin assessed by computer simulations. IUBMB Life. 2011;63(3):206-213.
http://dx.doi.org/10.1002/iub.444