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Carcagno, A.L.; Ogara, M.F.; Sonzogni, S.V.; Marazita, M.C.; Sirkin, P.F.; Ceruti, J.M.; Cánepa, E.T. "E2F1 transcription is induced by genotoxic stress through ATM/ATR activation" (2009) IUBMB Life. 61(5):537-543
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Abstract:

E2F1, a member of the E2F family of transcription factors, plays a critical role in controlling both cell cycle progression and apoptotic cell death in response to DNA damage and oncogene activation. Following genotoxic stresses, E2F1 protein is stabilized by phosphorylation and acetylation driven to its accumulation. The aim of the present work was to examine whether the increase in E2F1 protein levels observed after DNA damage is only a reflection of an increase in E2F1 protein stability or is also the consequence of enhanced transcription of the E2F1 gene. The data presented here demonstrates that UV light and other genotoxics induce the transcription of E2F1 gene in an ATM/ATR dependent manner, which results in increasing E2F1 mRNA and protein levels. After genotoxic stress, transcription of cyclin E, an E2F1 target gene, was significantly induced. This induction was the result of two well-differentiated effects, one of them dependent on de novo protein synthesis and the other on the protein stabilization. Our results strongly support a transcriptional effect of DNA damaging agents on E2F1 expression. The results presented herein uncover a new mechanism involving E2F1 in response to genotoxic stress. © 2009 IUBMB.

Registro:

Documento: Artículo
Título:E2F1 transcription is induced by genotoxic stress through ATM/ATR activation
Autor:Carcagno, A.L.; Ogara, M.F.; Sonzogni, S.V.; Marazita, M.C.; Sirkin, P.F.; Ceruti, J.M.; Cánepa, E.T.
Filiación:Departamento de Química Biológica, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Ciudad de Buenos Aires, Argentina
Palabras clave:ATM; ATR; Cyclin E; DNA damage response; E2F1; Genotoxic stress; Phosphorylation; Protein stability; Transcriptional regulation; UV irradiation; ATM protein; ATR protein; cyclin E; transcription factor E2F1; ATR protein, human; cell cycle protein; cyclin E; oligodeoxyribonucleotide; protein serine threonine kinase; transcription factor E2F1; article; controlled study; DNA damage; gene targeting; genetic transcription; genotoxicity; human; human cell; protein expression; protein phosphorylation; protein stability; protein synthesis; stress; cell line; DNA damage; genetics; metabolism; Northern blotting; phosphorylation; radiation exposure; transcription initiation; ultraviolet radiation; Western blotting; Blotting, Northern; Blotting, Western; Cell Cycle Proteins; Cell Line; Cyclin E; DNA Damage; E2F1 Transcription Factor; Humans; Oligodeoxyribonucleotides; Phosphorylation; Protein-Serine-Threonine Kinases; Transcriptional Activation; Ultraviolet Rays
Año:2009
Volumen:61
Número:5
Página de inicio:537
Página de fin:543
DOI: http://dx.doi.org/10.1002/iub.197
Título revista:IUBMB Life
Título revista abreviado:IUBMB Life
ISSN:15216543
CODEN:IULIF
CAS:ATR protein, human, 2.7.1.-; Cell Cycle Proteins; Cyclin E; E2F1 Transcription Factor; Oligodeoxyribonucleotides; Protein-Serine-Threonine Kinases, 2.7.11.1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15216543_v61_n5_p537_Carcagno

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Citas:

---------- APA ----------
Carcagno, A.L., Ogara, M.F., Sonzogni, S.V., Marazita, M.C., Sirkin, P.F., Ceruti, J.M. & Cánepa, E.T. (2009) . E2F1 transcription is induced by genotoxic stress through ATM/ATR activation. IUBMB Life, 61(5), 537-543.
http://dx.doi.org/10.1002/iub.197
---------- CHICAGO ----------
Carcagno, A.L., Ogara, M.F., Sonzogni, S.V., Marazita, M.C., Sirkin, P.F., Ceruti, J.M., et al. "E2F1 transcription is induced by genotoxic stress through ATM/ATR activation" . IUBMB Life 61, no. 5 (2009) : 537-543.
http://dx.doi.org/10.1002/iub.197
---------- MLA ----------
Carcagno, A.L., Ogara, M.F., Sonzogni, S.V., Marazita, M.C., Sirkin, P.F., Ceruti, J.M., et al. "E2F1 transcription is induced by genotoxic stress through ATM/ATR activation" . IUBMB Life, vol. 61, no. 5, 2009, pp. 537-543.
http://dx.doi.org/10.1002/iub.197
---------- VANCOUVER ----------
Carcagno, A.L., Ogara, M.F., Sonzogni, S.V., Marazita, M.C., Sirkin, P.F., Ceruti, J.M., et al. E2F1 transcription is induced by genotoxic stress through ATM/ATR activation. IUBMB Life. 2009;61(5):537-543.
http://dx.doi.org/10.1002/iub.197