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The realization that the mammalian proteomic complexity is achieved with a limited number of genes demands a better understanding of alternative splicing regulation. Promoter control of alternative splicing was originally described by our group in studies performed on the fibronectin gene. Recently, other labs extended our findings to the cystic fibrosis, CD44 and CGRP genes strongly supporting a coupling between transcription and pre-mRNA splicing. A possible mechanism that would fit in these results is that the promoter itself is responsible for recruiting splicing factors, such as SR proteins, to the site of transcription, possibly through transcription factors that bind the promoter or the transcriptional enhancers. An alternative model, discussed more extensively in this review, involves modulation of RNA pol II (pol II) elongation rate. The model is supported by findings that cis- and trans-acting factors that modulate pol II elongation on a particular template also provoke changes in the alternative splicing balance of the encoded mRNAs.


Documento: Artículo
Título:Control of alternative pre-mRNA splicing by RNA pol II elongation: Faster is not always better
Autor:Nogués, G.; Kadener, S.; Cramer, P.; De la Mata, M.; Fededa, J.P.; Blaustein, M.; Srebrow, A.; Kornblihtt, A.R.
Filiación:Depto. Fisiol., Biol. Molec./Cel., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina
Howard Hughes Medical Institute, Brandeis University, Waltham, MA 02454, United States
Harvard University, Dept. of Molecular and Cell Biology, 7 Divinity Avenue, Cambridge, MA 02138, United States
Palabras clave:Alternative splicing; Elongation; mRNA processing; RNA polymerase II; Transcription; calcitonin gene related peptide; fibronectin; Hermes antigen; messenger RNA; protein; proteome; transcription factor; alternative RNA splicing; animal cell; controlled study; cystic fibrosis; DNA template; gene control; gene function; genetic code; genetic transcription; mammalian genetics; nonhuman; promoter region; protein RNA binding; proteomics; regulatory mechanism; review; Alternative Splicing; Animals; Models, Genetic; RNA Polymerase II; RNA Precursors; RNA, Messenger; RNA-Binding Proteins; Transcription, Genetic; Animalia; Mammalia
Página de inicio:235
Página de fin:241
Título revista:IUBMB Life
Título revista abreviado:IUBMB Life
CAS:calcitonin gene related peptide, 83652-28-2; fibronectin, 86088-83-7; protein, 67254-75-5; RNA Polymerase II, EC 2.7.7.-; RNA Precursors; RNA, Messenger; RNA-Binding Proteins


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---------- APA ----------
Nogués, G., Kadener, S., Cramer, P., De la Mata, M., Fededa, J.P., Blaustein, M., Srebrow, A.,..., Kornblihtt, A.R. (2003) . Control of alternative pre-mRNA splicing by RNA pol II elongation: Faster is not always better. IUBMB Life, 55(4-5), 235-241.
---------- CHICAGO ----------
Nogués, G., Kadener, S., Cramer, P., De la Mata, M., Fededa, J.P., Blaustein, M., et al. "Control of alternative pre-mRNA splicing by RNA pol II elongation: Faster is not always better" . IUBMB Life 55, no. 4-5 (2003) : 235-241.
---------- MLA ----------
Nogués, G., Kadener, S., Cramer, P., De la Mata, M., Fededa, J.P., Blaustein, M., et al. "Control of alternative pre-mRNA splicing by RNA pol II elongation: Faster is not always better" . IUBMB Life, vol. 55, no. 4-5, 2003, pp. 235-241.
---------- VANCOUVER ----------
Nogués, G., Kadener, S., Cramer, P., De la Mata, M., Fededa, J.P., Blaustein, M., et al. Control of alternative pre-mRNA splicing by RNA pol II elongation: Faster is not always better. IUBMB Life. 2003;55(4-5):235-241.