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Estrada, L.C.; Roberti, M.J.; Simoncelli, S.; Levi, V.; Aramendía, P.F.; Martínez, O.E. "Detection of low quantum yield fluorophores and improved imaging times using metallic nanoparticles" (2012) Journal of Physical Chemistry B. 116(7):2306-2313
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Abstract:

The behavior of a fluorophore near a gold nanoparticle is rationalized by a theoretical description of the parameters that modify the fluorescence emission: nanoparticle-fluorophore distance, fluorescence quantum yield (φ0), and fluorophore absorption and emission spectra, to find optimum conditions for designing fluorophore-nanoparticle probes. The theoretical maximum gain in brightness of the nanoparticle-fluorophore system with respect to the isolated molecule increases almost inversely proportional to φ0. The brightness enhancement in imaging experiments in vitro was assessed by using Au-SiO2 core-shell nanoparticles deposited on glass. A ∼13-fold emission brightness enhancement for weakly fluorescent molecules was observed. A significant increase in fluorophore photostability, rendering longer imaging times, was obtained for fluorophores interacting with gold nanoparticles incorporated by endocytosis in cells. Our results illustrate a way to increase imaging times and to study molecules in the vicinity of a metallic nanoparticle after photobleaching of background fluorescence. © 2012 American Chemical Society.

Registro:

Documento: Artículo
Título:Detection of low quantum yield fluorophores and improved imaging times using metallic nanoparticles
Autor:Estrada, L.C.; Roberti, M.J.; Simoncelli, S.; Levi, V.; Aramendía, P.F.; Martínez, O.E.
Filiación:Departamento de Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
INQUIMAE-Departamento de Quimica Inorganica, Analitica y Quimica Fisica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, CA 92697, United States
Palabras clave:Emission spectroscopy; Fluorescence; Gold; Gold coatings; Luminance; Molecular biology; Molecules; Nanoparticles; Photobleaching; Quantum yield; Silicon compounds; Absorption and emission spectra; Core-shell nanoparticles; Fluorescence emission; Fluorescence quantum yield; Fluorescent molecules; Gold Nanoparticles; Imaging experiments; In-vitro; Isolated molecules; Maximum gain; Metallic nanoparticles; Optimum conditions; Photo-stability; Fluorophores
Año:2012
Volumen:116
Número:7
Página de inicio:2306
Página de fin:2313
DOI: http://dx.doi.org/10.1021/jp209467t
Título revista:Journal of Physical Chemistry B
Título revista abreviado:J Phys Chem B
ISSN:15206106
CODEN:JPCBF
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15206106_v116_n7_p2306_Estrada

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Citas:

---------- APA ----------
Estrada, L.C., Roberti, M.J., Simoncelli, S., Levi, V., Aramendía, P.F. & Martínez, O.E. (2012) . Detection of low quantum yield fluorophores and improved imaging times using metallic nanoparticles. Journal of Physical Chemistry B, 116(7), 2306-2313.
http://dx.doi.org/10.1021/jp209467t
---------- CHICAGO ----------
Estrada, L.C., Roberti, M.J., Simoncelli, S., Levi, V., Aramendía, P.F., Martínez, O.E. "Detection of low quantum yield fluorophores and improved imaging times using metallic nanoparticles" . Journal of Physical Chemistry B 116, no. 7 (2012) : 2306-2313.
http://dx.doi.org/10.1021/jp209467t
---------- MLA ----------
Estrada, L.C., Roberti, M.J., Simoncelli, S., Levi, V., Aramendía, P.F., Martínez, O.E. "Detection of low quantum yield fluorophores and improved imaging times using metallic nanoparticles" . Journal of Physical Chemistry B, vol. 116, no. 7, 2012, pp. 2306-2313.
http://dx.doi.org/10.1021/jp209467t
---------- VANCOUVER ----------
Estrada, L.C., Roberti, M.J., Simoncelli, S., Levi, V., Aramendía, P.F., Martínez, O.E. Detection of low quantum yield fluorophores and improved imaging times using metallic nanoparticles. J Phys Chem B. 2012;116(7):2306-2313.
http://dx.doi.org/10.1021/jp209467t