Abstract:
Nitrophorins are NO carrier proteins that transport and release NO through a pH-dependent conformational change. They bind NO tightly in a low pH environment and release it in a higher pH environment. Experimental evidence shows that the increase in the NO dissociation equilibrium constant, K d, is due mainly to an increase in the NO release rate. Structural and kinetic data strongly suggest that NPs control NO escape by modulating its migration from the active site to the solvent through a pH-dependent conformational change. NP2 and NP4 are two representative proteins of the family displaying a 39% overall sequence identity, and interestingly, NP2 releases NO slower than NP4. The proposal that NPs' NO release relies mainly on the NO escape rate makes NPs a very peculiar case among typical heme proteins. The connection between the pH-dependent conformational change and ligand release mechanism is not fully understood and the structural basis for the pH induced structural transition and the different NO release patterns in NPs are unresolved, yet interesting issues. In this work, we have used state of the art molecular dynamics simulations to study the NO escape process in NP2 and NP4 in both the low and high pH states. Our results show that both NPs modulate NO release by switching between a "closed" conformation in a low pH environment and an "open" conformation at higher pH. In both proteins, the change is caused by the differential protonation of a common residue Asp30 in NP4 and Asp29 in NP2, and the NO escape route is conserved. Finally, our results show that, in NP2, the conformational change to the "open" conformation is smaller than that for NP4 which results in a higher barrier for NO release. © 2009 American Chemical Society.
Registro:
Documento: |
Artículo
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Título: | pH-dependent mechanism of nitric oxide release in nitrophorins 2 and 4 |
Autor: | Swails, J.M.; Meng, Y.; Walker, F.A.; Marti, M.A.; Estrin, D.A.; Roitberg, A.E. |
Filiación: | Quantum Theory Project and Department of Chemistry, University of Florida, Gainesville, FL 32611-8435, United States Departments of Biochemistry and Chemistry, University of Arizona, Tucson, AZ 85721-0041, United States Departamento de Quimica Inorganica, Anaitica, y Quimica Fisica INQUIMAE-CONICET, Universidad de Buenos Aires, Ciudad Universitaria, Pab. II C1428EHA, Buenos Aires, Argentina Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pab. II C1428EHA, Buenos Aires, Argentina
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Palabras clave: | Dynamics; Equilibrium constants; Molecular dynamics; Nitric oxide; Porphyrins; Active sites; Carrier proteins; Conformational changes; Escape process; Escape rates; Escape routes; Experimental evidences; Heme proteins; High pH; Kinetic datum; Molecular dynamics simulations; Nitric oxide release; No dissociations; No release; pH dependents; Release mechanisms; Sequence identities; State-of-the arts; Structural basis; Structural transitions; Proteins |
Año: | 2009
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Volumen: | 113
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Número: | 4
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Página de inicio: | 1192
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Página de fin: | 1201
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DOI: |
http://dx.doi.org/10.1021/jp806906x |
Título revista: | Journal of Physical Chemistry B
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Título revista abreviado: | J Phys Chem B
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ISSN: | 15206106
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CODEN: | JPCBF
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15206106_v113_n4_p1192_Swails |
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Citas:
---------- APA ----------
Swails, J.M., Meng, Y., Walker, F.A., Marti, M.A., Estrin, D.A. & Roitberg, A.E.
(2009)
. pH-dependent mechanism of nitric oxide release in nitrophorins 2 and 4. Journal of Physical Chemistry B, 113(4), 1192-1201.
http://dx.doi.org/10.1021/jp806906x---------- CHICAGO ----------
Swails, J.M., Meng, Y., Walker, F.A., Marti, M.A., Estrin, D.A., Roitberg, A.E.
"pH-dependent mechanism of nitric oxide release in nitrophorins 2 and 4"
. Journal of Physical Chemistry B 113, no. 4
(2009) : 1192-1201.
http://dx.doi.org/10.1021/jp806906x---------- MLA ----------
Swails, J.M., Meng, Y., Walker, F.A., Marti, M.A., Estrin, D.A., Roitberg, A.E.
"pH-dependent mechanism of nitric oxide release in nitrophorins 2 and 4"
. Journal of Physical Chemistry B, vol. 113, no. 4, 2009, pp. 1192-1201.
http://dx.doi.org/10.1021/jp806906x---------- VANCOUVER ----------
Swails, J.M., Meng, Y., Walker, F.A., Marti, M.A., Estrin, D.A., Roitberg, A.E. pH-dependent mechanism of nitric oxide release in nitrophorins 2 and 4. J Phys Chem B. 2009;113(4):1192-1201.
http://dx.doi.org/10.1021/jp806906x