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Zaccaro, M.C.; Salazar, C.; De Caire, G.Z.; De Cano, M.S.; Stella, A.M. "Lead toxicity in cyanobacterial porphyrin metabolism" (2001) Environmental Toxicology. 16(1):61-67
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Abstract:

The effect of Pb2+ on growth, tetrapyrrole photosynthetic pigment content, total free porphyrin, and 5-aminolevulinate dehydratase (ALA-D) activity of a cyanobacterium, Microchaete tenera, and its ability to sequester Pb2+ from the culture medium were studied. Pb2+ was assayed by graphite furnace atomic absorption spectrophotometry. M. tenera growth and chlorophyll a content were not affected by 0.5, 1.0, and 6.0 ppm of Pb2+. These treatments doubled the protein content and increased the phycobiliprotein content by four times after 7 days. The ALA-D activity decreased in all concentrations by 63% at day 7 and by 34% at day 14. As a consequence of ALA-D inhibition, total free porphyrin also decreased by 64% at day 7 and by 40% at day 14. The highest biomass lead uptake (7454±565 μg Pb2+/g dry weight) was observed at day 3 with 6.0 ppm of Pb2+ in the culture medium. Uptake coefficient was highest (3723±279 μg Pb2+g-1 dry weight/ppm of applied Pb2+) with 1.0 ppm after 3 days. The increase in protein and antenna pigments on day 7 was probably a response to stress conditions and could explain why the toxic metal did not affect growth. ALA-D inhibition and high lead biomass content confirm the importance of this enzyme as a biological indicator for stress. © 2001 by John Wiley & Sons, Inc.

Registro:

Documento: Artículo
Título:Lead toxicity in cyanobacterial porphyrin metabolism
Autor:Zaccaro, M.C.; Salazar, C.; De Caire, G.Z.; De Cano, M.S.; Stella, A.M.
Filiación:Laboratorio de Fisiología Vegetal y Biología de Cyanobacteria, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2620 (1428), Buenos Aires, Argentina
Centro de Investigaciones Sobre Porfirinas y Porfirias (CIPYP), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Giraldes 2620 (1428), Buenos Aires, Argentina
Palabras clave:5-aminolevulinate dehydratase; Cyanobacteria; Lead biosorption; Lead toxicity; Tetrapyrrole pigments; bacterial protein; chlorophyll; graphite; lead; phycobiliprotein; porphobilinogen synthase; porphyrin; cyanobacterium; lead; toxicity; article; assay; atomic absorption spectrometry; bacterial growth; biomass; controlled study; culture medium; Cyanobacterium; enzyme activity; enzyme inhibition; lead poisoning; Microchaete tenera; molecular weight; nonhuman; photosynthesis; porphyrin metabolism; priority journal; stress; Cyanobacteria; Lead; Photosynthesis; Porphobilinogen Synthase; Porphyrins; Cyanobacteria; Microchaete tenera
Año:2001
Volumen:16
Número:1
Página de inicio:61
Página de fin:67
DOI: http://dx.doi.org/10.1002/1522-7278(2001)16:1<61::AID-TOX70>3.0.CO;2-L
Título revista:Environmental Toxicology
Título revista abreviado:Environ. Toxicol.
ISSN:15204081
CODEN:ETOXF
CAS:Lead, 7439-92-1; Porphobilinogen Synthase, EC 4.2.1.24; Porphyrins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15204081_v16_n1_p61_Zaccaro

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Citas:

---------- APA ----------
Zaccaro, M.C., Salazar, C., De Caire, G.Z., De Cano, M.S. & Stella, A.M. (2001) . Lead toxicity in cyanobacterial porphyrin metabolism. Environmental Toxicology, 16(1), 61-67.
http://dx.doi.org/10.1002/1522-7278(2001)16:1<61::AID-TOX70>3.0.CO;2-L
---------- CHICAGO ----------
Zaccaro, M.C., Salazar, C., De Caire, G.Z., De Cano, M.S., Stella, A.M. "Lead toxicity in cyanobacterial porphyrin metabolism" . Environmental Toxicology 16, no. 1 (2001) : 61-67.
http://dx.doi.org/10.1002/1522-7278(2001)16:1<61::AID-TOX70>3.0.CO;2-L
---------- MLA ----------
Zaccaro, M.C., Salazar, C., De Caire, G.Z., De Cano, M.S., Stella, A.M. "Lead toxicity in cyanobacterial porphyrin metabolism" . Environmental Toxicology, vol. 16, no. 1, 2001, pp. 61-67.
http://dx.doi.org/10.1002/1522-7278(2001)16:1<61::AID-TOX70>3.0.CO;2-L
---------- VANCOUVER ----------
Zaccaro, M.C., Salazar, C., De Caire, G.Z., De Cano, M.S., Stella, A.M. Lead toxicity in cyanobacterial porphyrin metabolism. Environ. Toxicol. 2001;16(1):61-67.
http://dx.doi.org/10.1002/1522-7278(2001)16:1<61::AID-TOX70>3.0.CO;2-L