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Teixeira, A.P.; Rangel, J.L.; Raniero, L.J.; Tosato, M.G.; Fávero, P.P.; Martin, A.A. "Confocal raman spectroscopy: Determination of natural moisturizing factor profile related to skin hydration" (2014) Revista Brasileira de Engenharia Biomedica. 30(1):11-16
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Abstract:

Introduction: Skin health and skin care to reduce the effects of aging are the main interests of many researchers. The skin is very important because it protects the body from various effects of the external environment, and studies of the largest organ of the human body have been conducted since antiquity. In skin, aging effects are severe enough to promote changes in cell structure and biochemical composition. In this study, we quantitatively analyzed the water content and natural moisturizing factor of human facial skin in vivo and in real time by confocal Raman spectroscopy. This non-invasive technique is capable of providing detailed information on the biochemical composition at different depth profiles in the skin. Methods: We studied 10 volunteers, phototype II (40 and 50 years old), using a confocal Raman system to examine the skin surface down to 25 μm. Raman spectra were obtained before product use (T0), and after 30 days of continuous use of cosmetics (T30). Results: The results show a significant increase of 6.4% in water content in the surface layer of the facial skin after the cosmetic use. The amounts of natural moisturizing factor (NMF) compounds were also increased. Urocanic acid underwent a greater change in relation to carboxylic acid pyrrolidone, with a 38.5% increase in the stratum corneum. Conclusion: Confocal Raman spectroscopy identified changes in the biochemical composition of the superficial layers of the epidermis, which suggests the anti-aging efficacy of the formulation.

Registro:

Documento: Artículo
Título:Confocal raman spectroscopy: Determination of natural moisturizing factor profile related to skin hydration
Autor:Teixeira, A.P.; Rangel, J.L.; Raniero, L.J.; Tosato, M.G.; Fávero, P.P.; Martin, A.A.
Filiación:Laboratório de Espectroscopia Vibracional Biomédica, Instituto de Pesquisa e Desenvolvimento - IP and D, Universidade do Vale do Paraíba - UNIVAP, Av. Shishima Hifumi, 2911, CEP 12244-000, São José dos Campos, SP, Brazil
Instituto de Química Física de los Materiales, Medio Ambiente y Energía - INQUIMAE, Universidad de Buenos Aires - UBA, Ciudad Universitaria, Pabellón II, C1428EHA, Buenos Aires, Argentina
Palabras clave:Confocal Raman spectroscopy; Cosmetics; NMF; Skin aging; Cosmetics; Skin; Biochemical composition; Confocal Raman spectroscopy; External environments; NMF; Noninvasive technique; Skin aging; Stratum corneum; Superficial layers; Hydration
Año:2014
Volumen:30
Número:1
Página de inicio:11
Página de fin:16
DOI: http://dx.doi.org/10.4322/rbeb.2014.002
Título revista:Revista Brasileira de Engenharia Biomedica
Título revista abreviado:Rev. Bras. Eng. Biomed.
ISSN:15173151
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15173151_v30_n1_p11_Teixeira

Referencias:

  • Alberti, I., Kalia, Y.N., Naik, A., Bonny, J.D., Guy, R.H., In vivo assessment of enhanced topical delivery of terbinafne to human stratum corneum (2001) Journal of Controlled Release: Official Journal of the Controlled Release Society, 71 (3), pp. 319-327. , http://dx.doi.org/10.1016/S0168-3659(01)00244-9
  • Bagatin, E., Envelhecimento cutâneo e o papel dos cosmecêuticos (2008) Boletim Dermatológico UNIFESP, 5 (17), pp. 1-4
  • Bergholt, M.S., Zheng, W., Huang, Z., Development of a multiplexing fingerprint and high wave number Raman spectroscopy technique for real-time in vivo tissue Raman measurements at endoscopy (2013) Journal of Biomedical Optics, 18 (3), p. 030502. , http://dx.doi.org/10.1117/1.JBO.18.3.030502
  • Bouwstra, J., Groenink, H., Wouter, W., Kempenaar, J.R., Stefan, G., Water distribution and natural moisturizer factor content in human skin equivalents are regulated by environmental relative humidity (2008) Journal of Investigative Dermatology, 128 (2), pp. 378-388
  • Caspers, P.J., Lucassen, G.W., Bruining, H., Puppels, G.J., Automated depth-scanning confocal Raman microspectrometer for rapid in vivo determination of water concentration profiles in human skin (2000) Journal of Raman Spectroscopy, 31, pp. 813-818. , http://dx.doi.org/10.1002/1097-4555(200008/09)31:8/9813::AID-JRS5733.0.CO;2-7
  • Caspers, P.J., Lucassen, G.W., Carter, E., Bruining, H., Puppels, G.J., In vivo confocal Raman microspectroscopy of the skin: Noninvasive determination of molecular concentration profles (2001) Journal of Investigative Dermatology, 116 (3), pp. 434-442. , http://dx.doi.org/10.1046/j.1523-1747.2001.01258.x
  • Caspers, P.J., Lucassen, G.W., Puppels, G.J., Combined in vivo confocal Raman spectroscopy and confocal microscopy of human skin (2003) Biophysical Journal, 85 (1), pp. 572-580. , http://dx.doi.org/10.1016/S0006-3495(03)74501-9
  • Chung, J.H., Seo, J.Y., Choi, H.R., Lee, M.K., Youn, C.S., Rhie, G., Cho, K.H., Eun, H.C., Modulation of skin collagen metabolism in aged and photoaged human skin in vivo (2001) Journal of Investigative Dermatology, 117 (5), pp. 1218-1224. , http://dx.doi.org/10.1046/j.0022-202x.2001.01544.x
  • Costa, A., (2012) Tratado Internacional De Cosmecêuticos, , Guanabara Koogan
  • de Carvalho, L.F.C.S., Sato, E.T., Almeida, J.D., Martinho, H.S., Diagnosis of infammatory lesions by high-wavenumber FT-Raman spectroscopy (2011) Theoretical Chemistry Accounts, 130 (4-6), pp. 221-1229. , http://dx.doi.org/10.1007/s00214-011-0972-2
  • Djabri, A., Guy, R.H., Delgado-Charro, M.B., Transdermal iontophoresis of ranitidine: An opportunity in paediatric drug therapy (2012) International Journal of Pharmaceutics, 435 (1), pp. 27-32. , http://dx.doi.org/10.1016/j.ijpharm.2012.03.006
  • Egawa, M., Hirao, T., Takahashi, M., In vivo estimation of stratum corneum thickness from water concentration profiles obtained with Raman spectroscopy (2007) Acta Dermato-Venereologica, 87 (1), pp. 4-8. , http://dx.doi.org/10.2340/00015555-0183
  • Fraser, S.J., Mulet, X., Martin, L., Praporski, S., Mechler, A., Hartley, P.G., Polyzos, A., Separovic, F., Surface immobilization of bio-functionalized cubosomes: Sensing of proteins by quartz crystal microbalance (2012) Langmuir: ACS Journal of Surfaces and Colloids, 28 (1), pp. 620-627. , http://dx.doi.org/10.1021/la2032994
  • Grayson, S., Elias, P.M., Isolation and lipid biochemical characterization of stratum corneum membrane complexes: Implications for the cutaneous permeability barrier (1982) Journal of Investigative Dermatology, 78 (2), pp. 128-135. , http://dx.doi.org/10.1111/1523-1747.ep12505953
  • Kezic, S., Kemperman, P.M.J.H., Koster, E.S., de Jongh, C.M., Thio, H.B., Campbell, L.E., Irvine, A.D., Caspers, P.J., Loss-of-function mutations in the flaggrin gene lead to reduced level of natural moisturizing factor in the stratum corneum (2008) Journal of Investigative Dermatology, 128 (8), pp. 2117-2119. , http://dx.doi.org/10.1038/jid.2008.29
  • Oliveira, P.K., Tosato, M.G., Alves, R.D.S., Martin, A.A., Skin biochemical composition analysis by Raman spectroscopy (2012) Revista Brasileira De Engenharia Biomédica, 28, pp. 278-287. , http://dx.doi.org/10.4322/rbeb.2012.032
  • Rawlings, A.V., Harding, C.R., Moisturization and skin barrier function (2004) Dermatologic Therapy, 17 (1), pp. 43-48. , http://dx.doi.org/10.1111/j.1396-0296.2004.04S1005.x
  • Tosato, M.G., Alves, R.S., Dos Santos, E.A.P., Raniero, L., Menezes, P.F.C., Belletti, K.M.S., Praes, C.E.O., Martin, A.A., Raman spectroscopic investigation of the effects of cosmetic formulations on the constituents and properties of human skin (2012) Photomedicine and Laser Surgery, 30 (2), pp. 85-91. , http://dx.doi.org/10.1089/pho.2011.3059
  • Wu, J.Q., Kilpatrick-Liverman, L., Characterizing the composition of underarm and forearm skin using confocal Raman spectroscopy (2011) International Journal of Cosmetic Science, 33 (3), pp. 257-262. , http://dx.doi.org/10.1111/j.1468-2494.2010.00627.x
  • Votano, J.R., Parham, M., Hall, L.H., Kier, L.B., Hall, L.M., Prediction of aqueous solubility based on large datasets using several QSPR models utilizing topological structure representation (2004) Chemistry & Biodiversity, pp. 1829-1841. , http://dx.doi.org/10.1002/cbdv.200490137

Citas:

---------- APA ----------
Teixeira, A.P., Rangel, J.L., Raniero, L.J., Tosato, M.G., Fávero, P.P. & Martin, A.A. (2014) . Confocal raman spectroscopy: Determination of natural moisturizing factor profile related to skin hydration. Revista Brasileira de Engenharia Biomedica, 30(1), 11-16.
http://dx.doi.org/10.4322/rbeb.2014.002
---------- CHICAGO ----------
Teixeira, A.P., Rangel, J.L., Raniero, L.J., Tosato, M.G., Fávero, P.P., Martin, A.A. "Confocal raman spectroscopy: Determination of natural moisturizing factor profile related to skin hydration" . Revista Brasileira de Engenharia Biomedica 30, no. 1 (2014) : 11-16.
http://dx.doi.org/10.4322/rbeb.2014.002
---------- MLA ----------
Teixeira, A.P., Rangel, J.L., Raniero, L.J., Tosato, M.G., Fávero, P.P., Martin, A.A. "Confocal raman spectroscopy: Determination of natural moisturizing factor profile related to skin hydration" . Revista Brasileira de Engenharia Biomedica, vol. 30, no. 1, 2014, pp. 11-16.
http://dx.doi.org/10.4322/rbeb.2014.002
---------- VANCOUVER ----------
Teixeira, A.P., Rangel, J.L., Raniero, L.J., Tosato, M.G., Fávero, P.P., Martin, A.A. Confocal raman spectroscopy: Determination of natural moisturizing factor profile related to skin hydration. Rev. Bras. Eng. Biomed. 2014;30(1):11-16.
http://dx.doi.org/10.4322/rbeb.2014.002