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Abstract:

P. caerulea is the Passiflora species with the widest distribution in Argentina. Despite the need to cultivate it to decrease the extraction impact on its natural populations, the information on how environmental factors affect seed germinability is scarce. In order to evaluate the greminative response and the role of the antioxidant metabolism against saline stress, freshly collected seeds were germinated at 26° C in the presence of 0 (control), 30, 60 or 90 mM NaCl. The percentage of germination, the content of soluble and carbonylated proteins, the activity of antioxidant enzymes and the generation of reactive oxygen species both in the pre-germination phase and during germination were evaluated. The removal of the aryl and the micropylar integument after 3 h imbibition proved to induce a rapid germination and activation of the enzymes catalase, glutathione S-transferase and peroxidases, together with an increase and a decrease in the generation of O2 - and H2O2, respectively, at the root apex. Even though the germination and activity of some of the studied enzymes decreased with the increase in external NaCl levels, when comparing populations of the control and 90 mM NaCl treatments having similar germination percentages, a marked induction of peroxidase activity was observed in the latter group. The proportion of carbonylated proteins did not differ among treatments, which suggests that, despite this species cannot be considered as halo-tolerant, the fact that the antioxidant metabolism efficiently contributed to prevent oxidative damage to the proteome may constitute an important mechanism to facilitate its establishment in environments with moderate salinity. © 2018 Museo Argentino de Ciencias Naturales Bernardino Rivadavia.

Registro:

Documento: Artículo
Título:Germinability and antioxidant metabolism in Passiflora caerulea L. seeds exposed to salt stress
Autor:Chaín, J.M.; Causin, H.F.
Filiación:Universidad de Buenos Aires. Consejo Nacional de Investigaciones Cientificas y Tecnicas, Instituto de Biodiversidad y Biologia Experimental y Aplicada (IBBEA), Ciudad Universitaria, Facultad de Ciencias Exactas y Naturales, Departamento de Biodiversidad y Biologia Experimental, Buenos Aires, C1428EHA, Argentina
Palabras clave:Catalase; Oxidative stress; Passiflora sp.; Peroxidise activity; Protein carbonylation; Sodium chloride
Año:2018
Volumen:20
Número:1
Página de inicio:23
Página de fin:34
DOI: http://dx.doi.org/10.22179/REVMACN.20.563
Título revista:Revista del Museo Argentino de Ciencias Naturales, Nueva Serie
Título revista abreviado:Rev. Museo Argentino Cienc. Nat. Nuev Serie
ISSN:15145158
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15145158_v20_n1_p23_Chain

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Citas:

---------- APA ----------
Chaín, J.M. & Causin, H.F. (2018) . Germinability and antioxidant metabolism in Passiflora caerulea L. seeds exposed to salt stress. Revista del Museo Argentino de Ciencias Naturales, Nueva Serie, 20(1), 23-34.
http://dx.doi.org/10.22179/REVMACN.20.563
---------- CHICAGO ----------
Chaín, J.M., Causin, H.F. "Germinability and antioxidant metabolism in Passiflora caerulea L. seeds exposed to salt stress" . Revista del Museo Argentino de Ciencias Naturales, Nueva Serie 20, no. 1 (2018) : 23-34.
http://dx.doi.org/10.22179/REVMACN.20.563
---------- MLA ----------
Chaín, J.M., Causin, H.F. "Germinability and antioxidant metabolism in Passiflora caerulea L. seeds exposed to salt stress" . Revista del Museo Argentino de Ciencias Naturales, Nueva Serie, vol. 20, no. 1, 2018, pp. 23-34.
http://dx.doi.org/10.22179/REVMACN.20.563
---------- VANCOUVER ----------
Chaín, J.M., Causin, H.F. Germinability and antioxidant metabolism in Passiflora caerulea L. seeds exposed to salt stress. Rev. Museo Argentino Cienc. Nat. Nuev Serie. 2018;20(1):23-34.
http://dx.doi.org/10.22179/REVMACN.20.563