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Abstract:

Background: Insulin and insulin-like growth factors (IGFs) act on tetrameric tyrosine kinase receptors controlling essential functions including growth, metabolism, reproduction and longevity. The insulin receptor (IR) binds insulin and IGFs with different affinities triggering different cell responses. Results: We showed that IGF-II induces cell proliferation and gene transcription when IR-B is over-expressed. We combined biotinylated ligands with streptavidin conjugated quantum dots and visible fluorescent proteins to visualize the binding of IGF-II and insulin to IR-B and their ensuing internalization. By confocal microscopy and flow cytometry in living cells, we studied the internalization kinetic through the IR-B of both IGF-II, known to elicit proliferative responses, and insulin, a regulator of metabolism. Conclusions: IGF-II promotes a faster internalization of IR-B than insulin. We propose that IGF-II differentially activates mitogenic responses through endosomes, while insulin-activated IR-B remains at the plasma membrane. This fact could facilitate the interaction with key effector molecules involved in metabolism regulation. © 2013 Giudice et al.; licensee BioMed Central Ltd.

Registro:

Documento: Artículo
Título:Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B
Autor:Giudice, J.; Barcos, L.S.; Guaimas, F.F.; Penas-Steinhardt, A.; Giordano, L.; Jares-Erijman, E.A.; Coluccio Leskow, F.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales (FCEN), Universidad de Buenos Aires (UBA), Buenos Aires, Argentina
Departamento de Química Orgánica, FCEN, CONICET, Buenos Aires, Argentina
Instituto de Estudios de la Inmunidad Humoral (IDEHU), CONICET-UBA y Cátedra de Inmunología, UBA, Buenos Aires, Argentina
Laboratory of Cellular Dynamics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany
Departamento de Ciencias Básicas, Universidad Nacional de Luján, Argentina
Department of Pathology and Immunology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, United States
Palabras clave:Endocytosis; Insulin receptor; Insulin/IGF-II; Microscopy; Quantum dots; Signaling; cyan fluorescent protein; insulin; insulin receptor; insulin receptor B; quantum dot; somatomedin B; streptavidin; unclassified drug; article; biotinylation; cell membrane; cell proliferation; confocal microscopy; controlled study; endocytosis; endosome; flow cytometry; gene overexpression; genetic transcription; human; human cell; internalization; metabolic regulation; mitogenesis; priority journal; protein interaction; receptor binding; receptor upregulation; signal transduction
Año:2013
Volumen:11
Número:1
DOI: http://dx.doi.org/10.1186/1478-811X-11-18
Título revista:Cell Communication and Signaling
Título revista abreviado:Cell Commun. Signal.
ISSN:1478811X
CAS:insulin, 9004-10-8; somatomedin B, 63774-77-6, 67763-97-7; streptavidin, 9013-20-1
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_1478811X_v11_n1_p_Giudice.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1478811X_v11_n1_p_Giudice

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Citas:

---------- APA ----------
Giudice, J., Barcos, L.S., Guaimas, F.F., Penas-Steinhardt, A., Giordano, L., Jares-Erijman, E.A. & Coluccio Leskow, F. (2013) . Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B. Cell Communication and Signaling, 11(1).
http://dx.doi.org/10.1186/1478-811X-11-18
---------- CHICAGO ----------
Giudice, J., Barcos, L.S., Guaimas, F.F., Penas-Steinhardt, A., Giordano, L., Jares-Erijman, E.A., et al. "Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B" . Cell Communication and Signaling 11, no. 1 (2013).
http://dx.doi.org/10.1186/1478-811X-11-18
---------- MLA ----------
Giudice, J., Barcos, L.S., Guaimas, F.F., Penas-Steinhardt, A., Giordano, L., Jares-Erijman, E.A., et al. "Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B" . Cell Communication and Signaling, vol. 11, no. 1, 2013.
http://dx.doi.org/10.1186/1478-811X-11-18
---------- VANCOUVER ----------
Giudice, J., Barcos, L.S., Guaimas, F.F., Penas-Steinhardt, A., Giordano, L., Jares-Erijman, E.A., et al. Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B. Cell Commun. Signal. 2013;11(1).
http://dx.doi.org/10.1186/1478-811X-11-18