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Abstract:

The role of cytosolic Ca2+ on the kinetics of Inositol 1,4,5-triphosphate receptors (IP3Rs) and on the dynamics of IP3R-mediated Ca2+ signals has been studied at large both experimentally and by modeling. The role of luminal Ca2+ has not been investigated with that much detail although it has been found that it is relevant for signal termination in the case of Ca2+ release through ryanodine receptors. In this work we present the results of observing the dynamics of luminal and cytosolic Ca2+ simultaneously in Xenopus laevis oocytes. Combining observations and modeling we conclude that there is a rapid mechanism that guarantees the availability of free Ca2+ in the lumen even when a relatively large Ca2+ release is evoked. Comparing the dynamics of cytosolic and luminal Ca2+ during a release, we estimate that they are consistent with a 80% of luminal Ca2+ being buffered. The rapid availability of free luminal Ca2+ correlates with the observation that the lumen occupies a considerable volume in several regions across the images. © 2016 IOP Publishing Ltd.

Registro:

Documento: Artículo
Título:Luminal Ca2+ dynamics during IP3R mediated signals
Autor:Lopez, L.F.; Dawson, S.P.
Filiación:DF, FCEN-UBA, Argentina
IFIBA, CONICET, Argentina
Palabras clave:calcium; fluorescent dye; inositol 1,4,5 trisphosphate receptor; animal; calcium signaling; chemistry; cytosol; metabolism; oocyte; Xenopus laevis; Animals; Calcium; Calcium Signaling; Cytosol; Fluorescent Dyes; Inositol 1,4,5-Trisphosphate Receptors; Oocytes; Xenopus laevis
Año:2016
Volumen:13
Número:3
DOI: http://dx.doi.org/10.1088/1478-3975/13/3/036006
Título revista:Physical Biology
Título revista abreviado:Phys. Biol.
ISSN:14783967
CAS:calcium, 7440-70-2, 14092-94-5; Calcium; Fluorescent Dyes; Inositol 1,4,5-Trisphosphate Receptors
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14783967_v13_n3_p_Lopez

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Citas:

---------- APA ----------
Lopez, L.F. & Dawson, S.P. (2016) . Luminal Ca2+ dynamics during IP3R mediated signals. Physical Biology, 13(3).
http://dx.doi.org/10.1088/1478-3975/13/3/036006
---------- CHICAGO ----------
Lopez, L.F., Dawson, S.P. "Luminal Ca2+ dynamics during IP3R mediated signals" . Physical Biology 13, no. 3 (2016).
http://dx.doi.org/10.1088/1478-3975/13/3/036006
---------- MLA ----------
Lopez, L.F., Dawson, S.P. "Luminal Ca2+ dynamics during IP3R mediated signals" . Physical Biology, vol. 13, no. 3, 2016.
http://dx.doi.org/10.1088/1478-3975/13/3/036006
---------- VANCOUVER ----------
Lopez, L.F., Dawson, S.P. Luminal Ca2+ dynamics during IP3R mediated signals. Phys. Biol. 2016;13(3).
http://dx.doi.org/10.1088/1478-3975/13/3/036006