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Abstract:

Background: BMP4 is a member of the transforming growth factor beta (TGFbeta) superfamily and Noggin is a potent BMP inhibitor that exerts its function by binding to BMPs preventing interactions with its receptors. The aim of this work was to investigate the role of BMP4 and Noggin, on oocytes in vitro maturation (m experiments) and embryos in vitro development (c experiments) of bovine.Methods: For m experiments, COCs were collected from slaughterhouse ovaries and in vitro matured in TCM with 100 ng/ml of either BMP4 or Noggin. After 24 h, the nuclear stage of the oocytes was determined by staining with Hoechst 33342. In addition, RT-qPCR was performed on MII oocytes to study the relative concentration of ZAR1, GDF9, BAX, MATER and HSP70 transcripts. Treated oocytes were submitted to parthenogenic activation (PA) or in vitro fertilization (IVF) and cultured in CR2. For c experiments, non-treated matured oocytes were submitted to PA or IVF to generate embryos that were exposed to 100 ng/ml of BMP4 or Noggin in CR2 until day nine of culture. Cleavage, blastocyst and hatching rates, expression pattern of the transcription factor Oct-4 in blastocysts and embryo cell number at day two and nine post-activation or fertilization were evaluated.Results: We found that Noggin, as BMP4, did not affect oocyte nuclear maturation. Noggin supplementation up-regulated the expression of HSP70 and MATER genes in matured oocytes. Moreover, BMP4 during maturation increased the proportion of Oct-4 positive cells in parthenogenic embryos. On the other hand, when Noggin was added to embryo culture medium, developmental rates of parthenogenic and in vitro fertilized embryos were reduced. However, BMP4 addition decreases the development only for in vitro fertilized embryos. BMP4 and Noggin during culture reduced the proportion of Oct-4-expressing cells.Conclusions: Our results show that BMP4 is implicated in bovine oocytes maturation and embryo development. Moreover, our findings demonstrate, for the first time, that a correct balance of BMP signaling is needed for proper pre-implantation development of bovine embryos. © 2011 La Rosa et al; licensee BioMed Central Ltd.

Registro:

Documento: Artículo
Título:Effects of bone morphogenic protein 4 (BMP4) and its inhibitor, Noggin, on in vitro maturation and culture of bovine preimplantation embryos
Autor:La Rosa, I.; Camargo, L.S.A.; Pereira, M.M.; Fernandez-Martin, R.; Paz, D.A.; Salamone, D.F.
Filiación:Laboratory of Animal Biotechnology, Agriculture Faculty, University of Buenos Aires (UBA), Argentina
Embrapa Dairy Cattle, Reproduction and Biotechnology, Dom Bosco, Juiz de Fora, M.G, Brazil
Laboratory of Developmental Biology BBED, Exact and Natural Sciences Faculty, UBA, IFIBYNE-CONICET, Argentina
National Institute of Scientific and Technological Research CONICET, Argentina
Palabras clave:bone morphogenetic protein 4; cell protein; growth differentiation factor 9; heat shock protein 70; mater protein; noggin; octamer transcription factor 4; protein Bax; transforming growth factor beta; unclassified drug; ZAR1 protein; autoantigen; carrier protein; heat shock protein 70; noggin protein; animal cell; article; blastocyst; cell nucleus; controlled study; cow; embryo culture; embryo development; fertilization in vitro; gene expression; hatching; immunocytochemistry; maturation; meiosis; nonhuman; oocyte maturation; parthenogenesis; preimplantation embryo; reverse transcription polymerase chain reaction; animal; biosynthesis; cattle; drug antagonism; drug effect; metabolism; physiology; Bovinae; Animals; Autoantigens; Blastocyst; Bone Morphogenetic Protein 4; Carrier Proteins; Cattle; Embryonic Development; Fertilization in Vitro; HSP70 Heat-Shock Proteins; Parthenogenesis
Año:2011
Volumen:9
DOI: http://dx.doi.org/10.1186/1477-7827-9-18
Título revista:Reproductive Biology and Endocrinology
Título revista abreviado:Reprod. Biol. Endocrinol.
ISSN:14777827
CAS:growth differentiation factor 9, 208778-50-1; carrier protein, 80700-39-6; Autoantigens; Bone Morphogenetic Protein 4; Carrier Proteins; HSP70 Heat-Shock Proteins; noggin protein, 148294-77-3
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14777827_v9_n_p_LaRosa

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Citas:

---------- APA ----------
La Rosa, I., Camargo, L.S.A., Pereira, M.M., Fernandez-Martin, R., Paz, D.A. & Salamone, D.F. (2011) . Effects of bone morphogenic protein 4 (BMP4) and its inhibitor, Noggin, on in vitro maturation and culture of bovine preimplantation embryos. Reproductive Biology and Endocrinology, 9.
http://dx.doi.org/10.1186/1477-7827-9-18
---------- CHICAGO ----------
La Rosa, I., Camargo, L.S.A., Pereira, M.M., Fernandez-Martin, R., Paz, D.A., Salamone, D.F. "Effects of bone morphogenic protein 4 (BMP4) and its inhibitor, Noggin, on in vitro maturation and culture of bovine preimplantation embryos" . Reproductive Biology and Endocrinology 9 (2011).
http://dx.doi.org/10.1186/1477-7827-9-18
---------- MLA ----------
La Rosa, I., Camargo, L.S.A., Pereira, M.M., Fernandez-Martin, R., Paz, D.A., Salamone, D.F. "Effects of bone morphogenic protein 4 (BMP4) and its inhibitor, Noggin, on in vitro maturation and culture of bovine preimplantation embryos" . Reproductive Biology and Endocrinology, vol. 9, 2011.
http://dx.doi.org/10.1186/1477-7827-9-18
---------- VANCOUVER ----------
La Rosa, I., Camargo, L.S.A., Pereira, M.M., Fernandez-Martin, R., Paz, D.A., Salamone, D.F. Effects of bone morphogenic protein 4 (BMP4) and its inhibitor, Noggin, on in vitro maturation and culture of bovine preimplantation embryos. Reprod. Biol. Endocrinol. 2011;9.
http://dx.doi.org/10.1186/1477-7827-9-18